Abstract
Variation in 30 chloroplast DNAs, representing 22 wild and cultivated accessions in the genus Pisum, was analyzed by comparing fragment patterns produced by 16 restriction endonucleases. Three types of mutations were detected. First, an inversion of between 2.2 kilobase pairs (kb) and 5.2 kb distinguished a population of P. humile from all other Pisum accessions examined. Second, deletions and insertions of between 50 and 1200 base pairs produced small restriction fragment length variations in four regions of the 120-kb chloroplast genome. Two of these regions—one of which is located within the sequence that is inverted in P. humile—showed a high degree of size polymorphism, to the extent that size differences were detected between individuals from the same accession. Finally, a total of only 11 restriction site mutations were detected among the 165 restriction sites sampled in the 30 DNAs. Based on these results and previous data, we conclude that the chloroplast genome is evolving very slowly relative to nuclear and mitochondrial DNAs. The Pisum chloroplast DNA restriction site mutations define two major lineages: One includes all tested accessions of P. fulvum, which is known to be cytogenetically quite distinct from all other Pisum taxa. The second includes 12 of 13 cultivated lines of the garden pea (P. sativum) and a wild population of P. humile from northern Israel. These observations strongly reinforce an earlier conclusion that the cultivated pea was domesticated primarily from northern populations of P. humile. A 13th P. sativum cultivar has a chloroplast genome that is significantly different from those of the aforementioned lines and somewhat more similar to those of P. elatius and southern populations of P. humile. This observation indicates that secondary hybridization may have occurred during the domestication of the garden pea.
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- Aquadro C. F., Greenberg B. D. Human mitochondrial DNA variation and evolution: analysis of nucleotide sequences from seven individuals. Genetics. 1983 Feb;103(2):287–312. doi: 10.1093/genetics/103.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Avise J. C., Giblin-Davidson C., Laerm J., Patton J. C., Lansman R. A. Mitochondrial DNA clones and matriarchal phylogeny within and among geographic populations of the pocket gopher, Geomys pinetis. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6694–6698. doi: 10.1073/pnas.76.12.6694. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Avise J. C., Lansman R. A., Shade R. O. The use of restriction endonucleases to measure mitochondrial DNA sequence relatedness in natural populations. I. Population structure and evolution in the genus Peromyscus. Genetics. 1979 May;92(1):279–295. doi: 10.1093/genetics/92.1.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fluhr R., Edelman M. Conservation of sequence arrangement among higher plant chloroplast DNAs: molecular cross hybridization among the Solanaceae and between Nicotiana and Spinacia. Nucleic Acids Res. 1981 Dec 21;9(24):6841–6853. doi: 10.1093/nar/9.24.6841. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palmer J. D., Edwards H., Jorgensen R. A., Thompson W. F. Novel evolutionary variation in transcription and location of two chloroplast genes. Nucleic Acids Res. 1982 Nov 11;10(21):6819–6832. doi: 10.1093/nar/10.21.6819. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palmer J. D., Thompson W. F. Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost. Cell. 1982 Jun;29(2):537–550. doi: 10.1016/0092-8674(82)90170-2. [DOI] [PubMed] [Google Scholar]
- Palmer J. D., Thompson W. F. Clone banks of the mung bean, pea and spinach chloroplast genomes. Gene. 1981 Oct;15(1):21–26. doi: 10.1016/0378-1119(81)90100-1. [DOI] [PubMed] [Google Scholar]
- Palmer J. D., Thompson W. F. Rearrangements in the chloroplast genomes of mung bean and pea. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5533–5537. doi: 10.1073/pnas.78.9.5533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Szostak J. W., Wu R. Unequal crossing over in the ribosomal DNA of Saccharomyces cerevisiae. Nature. 1980 Apr 3;284(5755):426–430. doi: 10.1038/284426a0. [DOI] [PubMed] [Google Scholar]
- Timothy D. H., Levings C. S., Pring D. R., Conde M. F., Kermicle J. L. Organelle DNA variation and systematic relationships in the genus Zea: Teosinte. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4220–4224. doi: 10.1073/pnas.76.9.4220. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsunewaki K., Ogihara Y. The Molecular Basis of Genetic Diversity among Cytoplasms of TRITICUM and AEGILOPS Species. II. on the Origin of Polyploid Wheat Cytoplasms as Suggested by Chloroplast DNA Restriction Fragment Patterns. Genetics. 1983 May;104(1):155–171. doi: 10.1093/genetics/104.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]