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. 1964 Feb;90(2):293–303. doi: 10.1042/bj0900293

The separation of synaptic vesicles from nerve-ending particles (`synaptosomes')

V P Whittaker 1, I A Michaelson 1,*, R Jeanette A Kirkland 1
PMCID: PMC1202615  PMID: 5834239

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALDRIDGE W. N., JOHNSON M. K. Cholinesterase, succinic dehydrogenase, nucleic acids, esterase and glutathione reductase in sub-cellular fractions from rat brain. Biochem J. 1959 Oct;73:270–276. doi: 10.1042/bj0730270. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERRY J. F., WHITTAKER V. P. The acyl-group specificity of choline acetylase. Biochem J. 1959 Nov;73:447–458. doi: 10.1042/bj0730447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DE ROBERTIS E. D., BENNETT H. S. Some features of the submicroscopic morphology of synapses in frog and earthworm. J Biophys Biochem Cytol. 1955 Jan;1(1):47–58. doi: 10.1083/jcb.1.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DE ROBERTIS E., RODRIGUEZ DE LORES ARNAIZ G., PELLEGRINO DE IRALDI A. Isolation of synaptic vesicles from nerve endings of the rat brain. Nature. 1962 May 26;194:794–795. doi: 10.1038/194794a0. [DOI] [PubMed] [Google Scholar]
  5. DE ROBERTIS E., RODRIGUEZ DE LORES ARNAIZ G., SALGANICOFF L., PELLEGRINO DE IRALDI A., ZIEHER L. M. Isolation of synaptic vesicles and structural organization of the acetycholine system within brain nerve endings. J Neurochem. 1963 Apr;10:225–235. doi: 10.1111/j.1471-4159.1963.tb05038.x. [DOI] [PubMed] [Google Scholar]
  6. DE ROBERTIS E., SALGANICOFF L., ZIEHER L. M., RODRIGUEZ DE LORES ARNAIZ G. Acetylcholine and cholinacetylase content of synaptic vesicles. Science. 1963 Apr 19;140(3564):300–301. doi: 10.1126/science.140.3564.300. [DOI] [PubMed] [Google Scholar]
  7. DE ROBERTIS E. Submicroscopic morphology and function of the synapse. Exp Cell Res. 1958;14(Suppl 5):347–369. [PubMed] [Google Scholar]
  8. DEL CASTILLO J., KATZ B. Biophysical aspects of neuro-muscular transmission. Prog Biophys Biophys Chem. 1956;6:121–170. [PubMed] [Google Scholar]
  9. DEL CASTILLO J., KATZ B. Local activity at a depolarized nerve-muscle junction. J Physiol. 1955 May 27;128(2):396–411. doi: 10.1113/jphysiol.1955.sp005315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FATT P., KATZ B. Spontaneous subthreshold activity at motor nerve endings. J Physiol. 1952 May;117(1):109–128. [PMC free article] [PubMed] [Google Scholar]
  11. HANZON V., TOSCHI G. Electron microscopy of microsomal fractions from rat brain. Exp Cell Res. 1959 Feb;16(2):256–271. doi: 10.1016/0014-4827(59)90253-8. [DOI] [PubMed] [Google Scholar]
  12. HEBB C. O., SMALLMAN B. N. Intracellular distribution of choline acetylase. J Physiol. 1956 Nov 28;134(2):385–392. doi: 10.1113/jphysiol.1956.sp005650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HEBB C. O., WHITTAKER V. P. Intracellular distributions of acetylcholine and choline acetylase. J Physiol. 1958 Jun 18;142(1):187–196. doi: 10.1113/jphysiol.1958.sp006008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HORNE R. W., WHITTAKER V. P. The use of the negative staining method for the electron-microscopic study of subcellular particles from animal tissues. Z Zellforsch Mikrosk Anat. 1962;58:1–16. doi: 10.1007/BF00406937. [DOI] [PubMed] [Google Scholar]
  15. JOHNSON M. K. The intracellular distribution of glycolytic and other enzymes in rat-brain homogenates and mitochondrial preparations. Biochem J. 1960 Dec;77:610–618. doi: 10.1042/bj0770610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. JOHNSON M. K., WHITTAKER V. P. LACTATE DEHYDROGENASE AS A CYTOPLASMIC MARKER IN BRAIN. Biochem J. 1963 Sep;88:404–409. doi: 10.1042/bj0880404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MICHAELSON I. A., WHITTAKER V. P. The distribution of hydroxytryptamine in brain fractions. Biochem Pharmacol. 1962 Jun;11:505–506. doi: 10.1016/0006-2952(62)90237-x. [DOI] [PubMed] [Google Scholar]
  18. MICHAELSON I. A., WHITTAKER V. P. The subcellular localization of 5-hydroxytryptamine in guinea pig brain. Biochem Pharmacol. 1963 Feb;12:203–211. doi: 10.1016/0006-2952(63)90185-0. [DOI] [PubMed] [Google Scholar]
  19. ROBERTSON J. D. The ultrastructure of a reptilian myoneural junction. J Biophys Biochem Cytol. 1956 Jul 25;2(4):381–394. doi: 10.1083/jcb.2.4.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. STRANG R. R., NORDENSTAM H. Dumb-bell ganglioneuroma of the cervical spine. Acta Neurol Scand. 1962;38:60–66. [PubMed] [Google Scholar]
  21. WHITTAKER V. P., GRAY E. G. The synapse: biology and morphology. Br Med Bull. 1962 Sep;18:223–228. doi: 10.1093/oxfordjournals.bmb.a069983. [DOI] [PubMed] [Google Scholar]
  22. WHITTAKER V. P., MICHAELSON I. A., KIRKLAND R. J. The separation of synaptic vesicles from disrupted nervending particles. Biochem Pharmacol. 1963 Mar;12:300–302. doi: 10.1016/0006-2952(63)90156-4. [DOI] [PubMed] [Google Scholar]
  23. WHITTAKER V. P. The isolation and characterization of acetylcholine-containing particles from brain. Biochem J. 1959 Aug;72:694–706. doi: 10.1042/bj0720694. [DOI] [PMC free article] [PubMed] [Google Scholar]

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