Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1986 Jul;113(3):745–754. doi: 10.1093/genetics/113.3.745

Genetic Differences among the a/J x C57bl/6j Recombinant Inbred Mouse Lines and Their Degree of Association with Glucocorticoid-Induced Cleft Palate

Sharon L Liu 1, Robert P Erickson 1
PMCID: PMC1202867  PMID: 3525322

Abstract

Hydrocortisone sodium phosphate was injected intramuscularly into A/J, C57BL/6J and recombinant inbred lines from these two parental lines to study the genetics of steroid-induced cleft palate in a situation of identical maternal and fetal genotypes. The strains were typed for H-2 (the major histocompatibility locus), β-glucuronidase and β2-microglobulin, which served as markers on chromosomes 17, 5 and 2, respectively. Hepatic glucocorticoid binding capacity had been previously measured in Hepes buffer and Hepes buffer plus dithiothreitol (DTT). The level of glucocorticoid binding in Hepes buffer and in Hepes plus DTT, as well as their ratio, was compared to the incidence of steroid-induced cleft palate in the recombinant inbred lines. A correlation was found between the response of glucocorticoid binding to DTT (expressed as a ratio of binding in the presence of DTT to binding without DTT) and hydrocortisone-induced cleft palate. When analyzing the effect of the three chromosomal markers on hydrocortisone-induced cleft palate, the b alleles of β2-microglobulin and of β-glucuronidase were associated with a higher incidence. Genetic analyses of the differences between these two inbred strains of mice in the incidence of steroid-induced cleft palate show it not to be monogenic.

Full Text

The Full Text of this article is available as a PDF (634.4 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey D. W. Recombinant-inbred strains. An aid to finding identity, linkage, and function of histocompatibility and other genes. Transplantation. 1971 Mar;11(3):325–327. doi: 10.1097/00007890-197103000-00013. [DOI] [PubMed] [Google Scholar]
  2. Biddle F. G., Fraser F. C. Genetics of cortisone-induced cleft palate in the mouse-embryonic and maternal effects. Genetics. 1976 Dec;84(4):743–754. doi: 10.1093/genetics/84.4.743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bonner J. J., Tyan M. L. Backcross test demonstrates the linkage of glucocorticoid-induced cleft palate susceptibility to H-2. Teratology. 1982 Oct;26(2):213–216. doi: 10.1002/tera.1420260214. [DOI] [PubMed] [Google Scholar]
  4. Erickson R. P., Butley M. S., Sing C. F. H-2 and non-H-2 determined strain variation in palatal shelf and tongue adenosine 3':5' cyclic monophosphate: a possible role in the etiology of steroid-induced cleft palate. J Immunogenet. 1979 Aug;6(4):253–262. doi: 10.1111/j.1744-313x.1979.tb00682.x. [DOI] [PubMed] [Google Scholar]
  5. Gasser D. L., Mele L., Lees D. D., Goldman A. S. Genes in mice that affect susceptibility to cortisone-induced cleft palate are closely linked to Ir genes on chromosomes 2 and 17. Proc Natl Acad Sci U S A. 1981 May;78(5):3147–3150. doi: 10.1073/pnas.78.5.3147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldman A. S., Baker M. K., Tomassini N., Hummeler K. Occurrence of cleft palate, micrognathia, and agnathia in selected strains of cortisone- and phenytoin-treated mice. J Craniofac Genet Dev Biol. 1982;2(4):277–284. [PubMed] [Google Scholar]
  7. Goldman A. S., Katsumata M., Yaffe S. J., Gassner D. L. Palatal cytosol cortisol-binding protein associated with cleft palate susceptibility and H-2 genotype. Nature. 1977 Feb 17;265(5595):643–645. doi: 10.1038/265643a0. [DOI] [PubMed] [Google Scholar]
  8. KALTER H., FRASER F. C. The modification of the teratogenic action of cortisone by parity. Science. 1953 Nov 20;118(3073):625–626. doi: 10.1126/science.118.3073.625. [DOI] [PubMed] [Google Scholar]
  9. KALTER H. Factors influencing the frequency of cortisone-induced cleft palate in mice. J Exp Zool. 1957 Apr;134(3):449–467. doi: 10.1002/jez.1401340304. [DOI] [PubMed] [Google Scholar]
  10. KALTER H. Modification of teratogenic action of cortisone in mice by maternal age, maternal weight and litter size. Am J Physiol. 1956 Apr;185(1):65–68. doi: 10.1152/ajplegacy.1956.185.1.65. [DOI] [PubMed] [Google Scholar]
  11. Liu S. L., Erickson R. P. Genetics of glucocorticoid receptor levels in recombinant inbred lines of mice. Genetics. 1986 Jul;113(3):735–744. doi: 10.1093/genetics/113.3.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Swank R. T., Bailey D. W. Recombinant inbred lines: value in the genetic analysis of biochemical variants. Science. 1973 Sep 28;181(4106):1249–1252. doi: 10.1126/science.181.4106.1249. [DOI] [PubMed] [Google Scholar]
  13. Tyan M. L., Miller K. K. Genetic and environmental factors in cortisone induced cleft palate. Proc Soc Exp Biol Med. 1978 Sep;158(4):618–621. doi: 10.3181/00379727-158-40259. [DOI] [PubMed] [Google Scholar]
  14. Vekemans M., Taylor B. A., Fraser F. C. The susceptibility to cortisone-induced cleft palate of recombinant inbred strains of mice: lack of association with the H-2 haplotype. Genet Res. 1981 Dec;38(3):327–331. doi: 10.1017/s0016672300020644. [DOI] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES