Abstract
Two alternate transcripts of the single copy Alcohol dehydrogenase (Adh) gene accumulate with developmental specificity in all of 12 species of Hawaiian picture-winged Drosophila which have been examined. Relative to the paradigm species D. affinidisjuncta, the Adh transcript normally restricted to larvae is found to accumulate in both larval and adult tissues in D. formella. The other Adh transcript, which normally accumulates only in adults, accumulates in third-instar D. prostopalpis larvae as well. In species hybrids, the D. formella phenotype shows additive inheritance. These observations document the existence of a novel type of genetic variability. Furthermore, such variants suggest specific properties for the biological systems that regulate ADH expression in Drosophila, and they should facilitate further experimental investigations.
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Selected References
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- Benyajati C., Spoerel N., Haymerle H., Ashburner M. The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages. Cell. 1983 May;33(1):125–133. doi: 10.1016/0092-8674(83)90341-0. [DOI] [PubMed] [Google Scholar]
- Berk A. J., Sharp P. A. Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274–1278. doi: 10.1073/pnas.75.3.1274. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brennan M. D., Rowan R. G., Dickinson W. J. Introduction of a functional P element into the germ-line of Drosophila hawaiiensis. Cell. 1984 Aug;38(1):147–151. doi: 10.1016/0092-8674(84)90535-x. [DOI] [PubMed] [Google Scholar]
- Brennan M. D., Rowan R. G., Rabinow L., Dickinson W. J. Isolation and initial characterization of the alcohol dehydrogenase gene from Drosophila affinidisjuncta. J Mol Appl Genet. 1984;2(5):436–446. [PubMed] [Google Scholar]
- Dickinson W. J., Carson H. L. Regulation of the tissue specificity of an enzyme by a cis-acting genetic element: evidence from interspecific Drosophila hybrids. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4559–4562. doi: 10.1073/pnas.76.9.4559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dickinson W. J. Tissue specificity of enzyme expression regulated by diffusible factors: evidence in Drosophila hybrids. Science. 1980 Feb 29;207(4434):995–997. doi: 10.1126/science.7352303. [DOI] [PubMed] [Google Scholar]
- Favaloro J., Treisman R., Kamen R. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol. 1980;65(1):718–749. doi: 10.1016/s0076-6879(80)65070-8. [DOI] [PubMed] [Google Scholar]
- Lusis A. J., Paigen K. Genetic determination of the alpha-galactosidase developmental program in mice. Cell. 1975 Nov;6(3):371–378. doi: 10.1016/0092-8674(75)90186-5. [DOI] [PubMed] [Google Scholar]
- PAIGEN K. The genetic control of enzyme activity during differentiation. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1641–1649. doi: 10.1073/pnas.47.10.1641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Robert B., Daubas P., Akimenko M. A., Cohen A., Garner I., Guenet J. L., Buckingham M. A single locus in the mouse encodes both myosin light chains 1 and 3, a second locus corresponds to a related pseudogene. Cell. 1984 Nov;39(1):129–140. doi: 10.1016/0092-8674(84)90198-3. [DOI] [PubMed] [Google Scholar]
- Schibler U., Hagenbüchle O., Wellauer P. K., Pittet A. C. Two promoters of different strengths control the transcription of the mouse alpha-amylase gene Amy-1a in the parotid gland and the liver. Cell. 1983 Jun;33(2):501–508. doi: 10.1016/0092-8674(83)90431-2. [DOI] [PubMed] [Google Scholar]
- Ursprung H., Leone J. Alcohol dehydrogenases: a polymorphism in Drosophila melanogaster. J Exp Zool. 1965 Nov;160(2):147–154. doi: 10.1002/jez.1401600202. [DOI] [PubMed] [Google Scholar]