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. 1991 Jul;128(3):513–520. doi: 10.1093/genetics/128.3.513

Interaction of the Yeast Omnipotent Suppressors Sup1(sup45) and Sup2(sup35) with Non-Mendelian Factors

A R Dagkesamanskaya 1, M D Ter-Avanesyan 1
PMCID: PMC1204525  PMID: 1874413

Abstract

The SUP1 and SUP2 genes code for protein factors intimately involved in the control of translational accuracy. The disrupted alleles of these genes confer a recessive lethal phenotype in both [psi(+)] and [psi(-)] genetic backgrounds, indicating an essential function for the corresponding proteins. In [psi(+)] diploids, heterozygous for the SUP1 null allele, several dominant phenotypes were evident with slow growth and inability to sporulate. These dominant phenotypes disappear after transformation with the multicopy plasmid carrying the wild-type allele of the SUP1 gene. Such dominant phenotypes were not observed for the SUP2 null allele. The incompatibility of multicopy plasmids carrying the SUP2 gene with guanidine hydrochloride-curable cytoplasmic factor(s) was also demonstrated. The possible mechanisms of interaction of the SUP1 and SUP2 genes with the [psi] determinant are discussed.

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Selected References

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  1. Alexander A., Sherman J., Horn D. Fundus fluorescein angiography: a summary of theoretical concepts and clinical applications. J Am Optom Assoc. 1979 Jan;50(1):53–63. [PubMed] [Google Scholar]
  2. All-Robyn J. A., Kelley-Geraghty D., Griffin E., Brown N., Liebman S. W. Isolation of omnipotent suppressors in an [eta+] yeast strain. Genetics. 1990 Mar;124(3):505–514. doi: 10.1093/genetics/124.3.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Atkinson N. S., Hopper A. K. Chromosome specificity of polysomy promotion by disruptions of the Saccharomyces cerevisiae RNA1 gene. Genetics. 1987 Jul;116(3):371–375. doi: 10.1093/genetics/116.3.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Breining P., Piepersberg W. Yeast omnipotent supressor SUP1 (SUP45): nucleotide sequence of the wildtype and a mutant gene. Nucleic Acids Res. 1986 Jul 11;14(13):5187–5197. doi: 10.1093/nar/14.13.5187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Broach J. R., Friedman L., Sherman F. Correspondence of yeast UAA suppressors to cloned tRNASerUCA genes. J Mol Biol. 1981 Aug 15;150(3):375–387. doi: 10.1016/0022-2836(81)90553-2. [DOI] [PubMed] [Google Scholar]
  7. Broach J. R., Strathern J. N., Hicks J. B. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene. 1979 Dec;8(1):121–133. doi: 10.1016/0378-1119(79)90012-x. [DOI] [PubMed] [Google Scholar]
  8. Chernov Iu O., Derkach I. L., Dagkesmanskaia A. R., Tikhomirova V. L., Ter-Avanesian M. D. Nonsens-supressiia pri amplifikatsii gena, kodiruiushchego belkovyi faktor transliatsii. Dokl Akad Nauk SSSR. 1988;301(5):1227–1229. [PubMed] [Google Scholar]
  9. Chevallier M. R., Bloch J. C., Lacroute F. Transcriptional and translational expression of a chimeric bacterial-yeast plasmid in yeasts. Gene. 1980 Oct;11(1-2):11–19. doi: 10.1016/0378-1119(80)90082-7. [DOI] [PubMed] [Google Scholar]
  10. Cox B. S. A recessive lethal super-suppressor mutation in yeast and other psi phenomena. Heredity (Edinb) 1971 Apr;26(2):211–232. doi: 10.1038/hdy.1971.28. [DOI] [PubMed] [Google Scholar]
  11. Eustice D. C., Wakem L. P., Wilhelm J. M., Sherman F. Altered 40 S ribosomal subunits in omnipotent suppressors of yeast. J Mol Biol. 1986 Mar 20;188(2):207–214. doi: 10.1016/0022-2836(86)90305-0. [DOI] [PubMed] [Google Scholar]
  12. Goodman H. M., Olson M. V., Hall B. D. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453–5457. doi: 10.1073/pnas.74.12.5453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hawthorne D. C., Leupold U. Suppressors in yeast. Curr Top Microbiol Immunol. 1974;64(0):1–47. doi: 10.1007/978-3-642-65848-8_1. [DOI] [PubMed] [Google Scholar]
  14. Himmelfarb H. J., Maicas E., Friesen J. D. Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product. Mol Cell Biol. 1985 Apr;5(4):816–822. doi: 10.1128/mcb.5.4.816. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ito H., Fukuda Y., Murata K., Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. doi: 10.1128/jb.153.1.163-168.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kikuchi Y., Shimatake H., Kikuchi A. A yeast gene required for the G1-to-S transition encodes a protein containing an A-kinase target site and GTPase domain. EMBO J. 1988 Apr;7(4):1175–1182. doi: 10.1002/j.1460-2075.1988.tb02928.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kushnirov V. V., Ter-Avanesyan M. D., Didichenko S. A., Smirnov V. N., Chernoff Y. O., Derkach I. L., Novikova O. N., Inge-Vechtomov S. G., Neistat M. A., Tolstorukov I. I. Divergence and conservation of SUP2 (SUP35) gene of yeast Pichia pinus and Saccharomyces cerevisiae. Yeast. 1990 Nov-Dec;6(6):461–472. doi: 10.1002/yea.320060603. [DOI] [PubMed] [Google Scholar]
  18. Kushnirov V. V., Ter-Avanesyan M. D., Telckov M. V., Surguchov A. P., Smirnov V. N., Inge-Vechtomov S. G. Nucleotide sequence of the SUP2 (SUP35) gene of Saccharomyces cerevisiae. Gene. 1988 Jun 15;66(1):45–54. doi: 10.1016/0378-1119(88)90223-5. [DOI] [PubMed] [Google Scholar]
  19. Liebman S. W., Sherman F. Extrachromosomal psi+ determinant suppresses nonsense mutations in yeast. J Bacteriol. 1979 Sep;139(3):1068–1071. doi: 10.1128/jb.139.3.1068-1071.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Liebman S. W., Stewart J. W., Sherman F. Serine substitutions caused by an ochre suppressor in yeast. J Mol Biol. 1975 Jun 5;94(4):595–610. doi: 10.1016/0022-2836(75)90324-1. [DOI] [PubMed] [Google Scholar]
  21. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  22. Masurekar M., Palmer E., Ono B. I., Wilhelm J. M., Sherman F. Misreading of the ribosomal suppressor SUP46 due to an altered 40 S subunit in yeast. J Mol Biol. 1981 Apr 15;147(3):381–390. doi: 10.1016/0022-2836(81)90490-3. [DOI] [PubMed] [Google Scholar]
  23. Ono B. I., Stewart J. W., Sherman F. Serine insertion caused by the ribosomal suppressor SUP46 in yeast. J Mol Biol. 1981 Apr 15;147(3):373–379. doi: 10.1016/0022-2836(81)90489-7. [DOI] [PubMed] [Google Scholar]
  24. Pocklington M. J., Johnston L., Jenkins J. R., Orr E. The omnipotent suppressor SUP45 affects nucleic acid metabolism and mitochondrial structure. Yeast. 1990 Sep-Oct;6(5):441–450. doi: 10.1002/yea.320060509. [DOI] [PubMed] [Google Scholar]
  25. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
  26. Struhl K., Stinchcomb D. T., Scherer S., Davis R. W. High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035–1039. doi: 10.1073/pnas.76.3.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Surguchov A. P., Beretetskaya Y. V., Fominykch E. S., Pospelova E. M., SmirnovVN, Ter-Avanesyan M. D., Inge-Vechtomov S. G. Recessive suppression in yeast Saccharomyces cerevisiae is mediated by a ribosomal mutation. FEBS Lett. 1980 Feb 25;111(1):175–178. doi: 10.1016/0014-5793(80)80786-1. [DOI] [PubMed] [Google Scholar]
  28. Tuite M. F., Mundy C. R., Cox B. S. Agents that cause a high frequency of genetic change from [psi+] to [psi-] in Saccharomyces cerevisiae. Genetics. 1981 Aug;98(4):691–711. doi: 10.1093/genetics/98.4.691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wilson P. G., Culbertson M. R. SUF12 suppressor protein of yeast. A fusion protein related to the EF-1 family of elongation factors. J Mol Biol. 1988 Feb 20;199(4):559–573. doi: 10.1016/0022-2836(88)90301-4. [DOI] [PubMed] [Google Scholar]

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