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. 1965 Feb;94(2):502–508. doi: 10.1042/bj0940502

Studies on a glycopeptide from ovalbumin

J R Clamp 1,*, L Hough 1
PMCID: PMC1206535  PMID: 14346091

Abstract

1. The structure of the carbohydrate component of the glycopeptide isolated from the proteolytic digest of ovalbumin has been investigated by chemical and enzymic methods. 2. The results are consistent with the presence of a single carbohydrate prosthetic group, linked through its reducing end group to the peptide chain. 3. Further, all the 2-amino-2-deoxy-d-glucose units appear to be in the N-acylated form, the phenolic hydroxyl group of tyrosine is free and the ω-carboxyl group of aspartic acid is substituted. 4. The carbohydrate component has a branched-chain structure, the two non-reducing ends being terminated by a d-mannopyranosyl and a 2-acetamido-2-deoxy-d-glucopyranosyl residue respectively. 5. The terminal d-mannopyranosyl unit is probably linked through at least one other d-mannopyranosyl residue to the remainder of the carbohydrate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON J. D., ANDREWS P., HOUGH L. The biosynthesis and metabolism of polyols. Sorbitol (D-glucitol) of plum leaves. Biochem J. 1961 Oct;81:149–154. doi: 10.1042/bj0810149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  3. Bragg P. D., Hough L. An investigation of the egg-white mucoproteins, ovomucoid and ovalbumin. Biochem J. 1961 Jan;78(1):11–23. doi: 10.1042/bj0780011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CLAMP J. R., HOUGH L. THE PERIODATE OXIDATION OF AMINO ACIDS WITH REFERENCE TO STUDIES ON GLYCOPROTEINS. Biochem J. 1965 Jan;94:17–24. doi: 10.1042/bj0940017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CLAMP J. R., PUTNAM F. W. THE CARBOHYDRATE PROSTHETIC GROUP OF HUMAN GAMMA-GLOBULIN. J Biol Chem. 1964 Oct;239:3233–3240. [PubMed] [Google Scholar]
  6. CUNNINGHAM L. W., NUENKE B. J., NUENKE R. B. Preparation of glycopeptides from ovalbumin. Biochim Biophys Acta. 1957 Dec;26(3):660–661. doi: 10.1016/0006-3002(57)90126-9. [DOI] [PubMed] [Google Scholar]
  7. Dent C. E. A study of the behaviour of some sixty amino-acids and other ninhydrin-reacting substances on phenol-;collidine' filter-paper chromatograms, with notes as to the occurrence of some of them in biological fluids. Biochem J. 1948;43(2):169–180. [PMC free article] [PubMed] [Google Scholar]
  8. Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FLETCHER A. P., MARKS G. S., MARSHALL R. D., NEUBERGER A. Carbohydrates in protein. 5. Procedures for the isolation of glycopeptides from hen's-egg albumin and their oxidation by periodate. Biochem J. 1963 May;87:265–273. doi: 10.1042/bj0870265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Goodwin T. W., Morton R. A. The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochem J. 1946;40(5-6):628–632. doi: 10.1042/bj0400628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. JEVONS F. R. Linkage of carbohydrate to protein in ovalbumin. Nature. 1958 May 10;181(4619):1346–1347. doi: 10.1038/1811346a0. [DOI] [PubMed] [Google Scholar]
  12. JOHANSEN P. G., MARSHALL R. D., NEUBERGER A. Carbohydrates in protein. 3 The preparation and some of the properties of a glycopeptide from hen's-egg albumin. Biochem J. 1961 Mar;78:518–527. doi: 10.1042/bj0780518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. JOHANSEN P., MARSHALL R. D., NEUBERGER A. Carbohydrate peptide complex from egg albumin. Nature. 1958 May 10;181(4619):1345–1346. doi: 10.1038/1811345b0. [DOI] [PubMed] [Google Scholar]
  14. MOORE S., STEIN W. H. Chromatography of amino acids on sulfonated polystyrene resins. J Biol Chem. 1951 Oct;192(2):663–681. [PubMed] [Google Scholar]
  15. Marks G. S., Marshall R. D., Neuberger A. Carbohydrates in protein. 6. Studies on the carbohydrate-peptide bond in hen's-egg albumin. Biochem J. 1963 May;87(2):274–281. doi: 10.1042/bj0870274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morgan W. T., Elson L. A. A colorimetric method for the determination of N-acetylglucosamine and N-acetylchrondrosamine. Biochem J. 1934;28(3):988–995. doi: 10.1042/bj0280988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NUENKE R. H., CUNNINGHAM L. W. Preparation and structural studies of ovalbumin glycopeptides. J Biol Chem. 1961 Sep;236:2452–2460. [PubMed] [Google Scholar]
  18. Neuberger A. Carbohydrates in protein: The carbohydrate component of crystalline egg albumin. Biochem J. 1938 Sep;32(9):1435–1451. doi: 10.1042/bj0321435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Partridge S. M. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. doi: 10.1042/bj0420238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. RAMSAY J. A. A new method of freezing-point determination for small quantities. J Exp Biol. 1949 May;26(1):57–64. doi: 10.1242/jeb.26.1.57. [DOI] [PubMed] [Google Scholar]
  21. ROTHFUS J. A., SMITH E. L. Glycopeptides. IV. The periodate oxidation of glycopeptides from human gamma-globulin. J Biol Chem. 1963 Apr;238:1402–1410. [PubMed] [Google Scholar]
  22. SCHMID K., BENCZE W. L., NUSSBAUMER T., WEHRMULLER J. O. Studies on the structure of alpha-1-acid glycoprotein. J Biol Chem. 1959 Mar;234(3):529–533. [PubMed] [Google Scholar]
  23. WHISTLER R. L., BEMILLER J. N. Alkaline degradation of polysaccharides. Adv Carbohydr Chem. 1958;13:289–329. doi: 10.1016/s0096-5332(08)60359-8. [DOI] [PubMed] [Google Scholar]
  24. YAMASHINA I., MAKINO M. The properties of a carbohydrate-amino acid complex from ovalbumin. J Biochem. 1962 May;51:359–364. [PubMed] [Google Scholar]

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