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. 1965 Mar;94(3):518–524. doi: 10.1042/bj0940518

Fractionation of thymidine phosphokinase, thymidine 5′-monophosphate phosphokinase and thymidine 5′-diphosphate phosphokinase in extracts of Landschutz ascites-tumour cells

H J Grav 1,*, R M S Smellie 1
PMCID: PMC1206583  PMID: 14340042

Abstract

1. Extracts of Landschutz ascites-tumour cells have been fractionated by treatment with acid, alumina Cγ gel and Sephadex G-100 to yield purified preparations of thymidine phosphokinase, thymidine 5′-monophosphate phosphokinase and thymidine 5′-diphosphate phosphokinase. 2. These results clearly demonstrate the existence in Landschutz ascites tumour of three phosphokinases each of which catalyses one step in the reaction sequence: thymidine⇌thymidine 5′-monophosphate⇌thymidine 5′-diphosphate⇌thymidine 5′-triphosphate. Though these results do not preclude the participation of other enzymes in the formation of thymidine 5′-triphosphate from thymidine by Landschutz ascites-tumour cells, they provide strong support for the view that thymidine 5′-diphosphate is an intermediate in the formation of thymidine 5′-triphosphate from thymidine 5′-monophosphate by this system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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