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. 1965 Mar;94(3):687–691. doi: 10.1042/bj0940687

The reduction of inorganic sulphate to inorganic sulphite in the small intestine of the rat

H C Robinson 1,*
PMCID: PMC1206603  PMID: 14340059

Abstract

1. Whole scrapings of rat intestinal mucosa were incubated with carrier-free sodium [35S]sulphate. Radioactivity was found in S-sulphocysteine and to a small extent in S-sulphoglutathione. 2. Whole scrapings of rat intestinal mucosa incubated with carrier-free sodium [35S]sulphate and oxidized glutathione formed S[35S]-sulphoglutathione as the main radioactive product. The amount of S[35S]-sulphocysteine formed was considerably lower than in a control that contained no oxidized glutathione. 3. The supernatant fraction of homogenates of rat intestinal mucosa catalyses the NADPH-dependent reduction of adenosine 3′-phosphate 5′-sulphatophosphate to inorganic sulphite. NADH or GSH fail to replace NADPH as reducing agents. 4. The formation of inorganic [35S]sulphite from inorganic [35S]-sulphate may account for the incorporation of [35S]sulphate into S-sulphoglutathione by the small intestine of the rat in vivo and in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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