Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1965 Mar;94(3):687–691. doi: 10.1042/bj0940687

The reduction of inorganic sulphate to inorganic sulphite in the small intestine of the rat

H C Robinson 1,*
PMCID: PMC1206603  PMID: 14340059

Abstract

1. Whole scrapings of rat intestinal mucosa were incubated with carrier-free sodium [35S]sulphate. Radioactivity was found in S-sulphocysteine and to a small extent in S-sulphoglutathione. 2. Whole scrapings of rat intestinal mucosa incubated with carrier-free sodium [35S]sulphate and oxidized glutathione formed S[35S]-sulphoglutathione as the main radioactive product. The amount of S[35S]-sulphocysteine formed was considerably lower than in a control that contained no oxidized glutathione. 3. The supernatant fraction of homogenates of rat intestinal mucosa catalyses the NADPH-dependent reduction of adenosine 3′-phosphate 5′-sulphatophosphate to inorganic sulphite. NADH or GSH fail to replace NADPH as reducing agents. 4. The formation of inorganic [35S]sulphite from inorganic [35S]-sulphate may account for the incorporation of [35S]sulphate into S-sulphoglutathione by the small intestine of the rat in vivo and in vitro.

Full text

PDF
687

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHAPEVILLE F., FROMAGEOT P. Formation de sulfite, d'acide cystéique et de taurine à partir de sulfate par l'oeuf embryonné. Biochim Biophys Acta. 1957 Dec;26(3):538–558. doi: 10.1016/0006-3002(57)90102-6. [DOI] [PubMed] [Google Scholar]
  2. MOORE S., STEIN W. H. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J Biol Chem. 1954 Dec;211(2):907–913. [PubMed] [Google Scholar]
  3. NOSE Y., LIPMANN F. Separation of steroid sulfokinases. J Biol Chem. 1958 Dec;233(6):1348–1351. [PubMed] [Google Scholar]
  4. PASTERNAK C. A., KENT P. W., DAVIES R. E. Biosynthesis of intestinal mucins. 1. Survey of incorporation of [35S] sulphate by isolated gastrointestinal tissues. Biochem J. 1958 Feb;68(2):212–217. doi: 10.1042/bj0680212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. PASTERNAK C. A. The synthesis of 3'-phosphoadenosine 5'-phosphosulfate by mouse tissue: sulfate activation in vitro and in vivo. J Biol Chem. 1960 Feb;235:438–442. [PubMed] [Google Scholar]
  6. Robinson H. C., Pasternak C. A. The isolation of S-sulphoglutathione from the small intestine of the rat. Biochem J. 1964 Dec;93(3):487–492. doi: 10.1042/bj0930487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. WILSON L. G., ASAHI T., BANDURSKI R. S. Yeast sulfate-reducing system. I. Reduction of sulfate to sulfite. J Biol Chem. 1961 Jun;236:1822–1829. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES