Abstract
1. The branched-chain pentose apiose reacts with the benzidine–trichloroacetic acid reagent on paper chromatograms to give a yellow spot with·an intense white fluorescence in ultraviolet light; on chromatograms developed with butanol–acetic acid–water this spot lies between fucose and rhamnose. 2. Examination of paper chromatograms of hydrolysates of whole plant material has shown the presence of a substance with these properties in a wide variety of species. 3. Among the plants examined two members of the Lemnaceae (Lemna minor and Wolffia arrhiza) were found to be especially rich sources, comparable with Posidonia australis (Bell, Hardwick, Isherwood & Cahn, 1954). 4. Measurements of the apiose content of fractions derived from Lemna have shown that the sugar is present at a concentration of about 4% in the holocellulose, and part of this is retained in the α-cellulose left by extraction with 24% (w/v) potassium hydroxide containing borate.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BACON J. S. D., EDELMAN J. The carbohydrates of the Jerusalem artichoke and other Compositae. Biochem J. 1951 Jan;48(1):114–126. doi: 10.1042/bj0480114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DUFF R. B., EASTWOOD D. J. Use of the arsenomolybdate-somogyi reagent in quantitative paper chromatography, and its application to the study of sucrose utilization by a fungus. Nature. 1950 May 27;165(4204):848–849. doi: 10.1038/165848b0. [DOI] [PubMed] [Google Scholar]
- LAIDLAW R. A., REID S. G. Filter-paper chromatography: extraction of sugars from the paper at room temperature. Nature. 1950 Sep 16;166(4220):476–477. doi: 10.1038/166476b0. [DOI] [PubMed] [Google Scholar]
- Partridge S. M. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall. Biochem J. 1948;42(2):238–250. doi: 10.1042/bj0420238. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]