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. 1965 Jun;95(3):661–668. doi: 10.1042/bj0950661

The photo-oxidation of succinate by chromatophores of Rhodospirillum rubrum

M C W Evans 1
PMCID: PMC1206791  PMID: 14342500

Abstract

1. The stoicheiometry of the photo-oxidation of succinate by chromatophores has been investigated with [2,3-14C2]succinate. It was found that there is a stoicheiometric relationship between the amount of succinate oxidized and the NAD reduced, and that fumarate is the only product of succinate oxidation. 2. The possibility of a direct hydrogen transfer from succinate to NAD in this reaction was investigated with tritiated substrates. With tritiated succinate less than 3% of the activity expected if direct hydrogen transfer occurred was recovered in the NADH2, and this was due to contamination with the substrate. In experiments with tritiated water, NADH2 was labelled, and had half the specific activity of the water, as expected if water was the source of protons. It was also found that chromatophores catalyse an exchange reaction between NADH2 and water. 3. It is concluded that the exchange reaction makes it impossible to interpret these results as indicating either a hydrogen-transfer or an electron-transfer mechanism for the photoreduction reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APPLEBY C. A., MORTON R. K. Lactic dehydrogenase and cytochrome b2 of baker's yeast; purification and crystallization. Biochem J. 1959 Mar;71(3):492–499. doi: 10.1042/bj0710492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ARNON D. I. Conversion of light into chemical energy in photosynthesis. Nature. 1959 Jul 4;184:10–21. doi: 10.1038/184010a0. [DOI] [PubMed] [Google Scholar]
  3. Allen R. J. The estimation of phosphorus. Biochem J. 1940 Jun;34(6):858–865. doi: 10.1042/bj0340858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BOERI E., CUTOLO E., LUZZATI M., TOSI L. Preparation and properties of cytochrome b2 from yeast. Arch Biochem Biophys. 1955 Jun;56(2):487–499. doi: 10.1016/0003-9861(55)90269-1. [DOI] [PubMed] [Google Scholar]
  5. BOSE S. K., GEST H. Bacterial photophosphorylation: regulation by redox balance. Proc Natl Acad Sci U S A. 1963 Mar 15;49:337–345. doi: 10.1073/pnas.49.3.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. ELSDEN S. R., ORMEROD J. G. The effect of monofluoroacetate on the metabolism of Rhodospirillum rubrum. Biochem J. 1956 Aug;63(4):691–701. doi: 10.1042/bj0630691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GELLER D. M., LIPMANN F. Photophosphorylation in extracts of Rhodospirillum rubrum. J Biol Chem. 1960 Aug;235:2478–2484. [PubMed] [Google Scholar]
  8. KNIGHT M. The photometabolism of propionate by Rhodospirillum rubrum. Biochem J. 1962 Jul;84:170–185. doi: 10.1042/bj0840170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LASCELLES J. The synthesis of porphyrins and bacteriochlorophyll by cell suspensions of Rhodopseudomonas spheroides. Biochem J. 1956 Jan;62(1):78–93. doi: 10.1042/bj0620078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moses V., Calvin M. THE PATH OF CARBON IN PHOTOSYNTHESIS. XXII. THE IDENTIFICATION OF CARBOXY-KETOPENTITOL DIPHOSPHATES AS PRODUCTS OF PHOTOSYNTHESIS. Proc Natl Acad Sci U S A. 1958 Mar;44(3):260–277. doi: 10.1073/pnas.44.3.260. [DOI] [PMC free article] [PubMed] [Google Scholar]

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