Abstract
1. A description is given of the isolation of a glycoprotein from hen's-egg white; it has been called ovoglycoprotein. 2. It contains 13·6% of hexose, 13·8% of hexosamine and 3% of sialic acid. 3. Hexose occurs as mannose and galactose in the ratio 2:1, hexosamine as glucosamine and sialic acid as N-acetylneuraminic acid. 4. It has S20,w 2·47s and a minimum molecular weight, calculated from the tryptophan content, of 24400. 5. At pH3·9 in acetate buffer, I0·1, which is in the isoelectric region, it is resolved into two components, one of which moves slowly towards the anode while the other moves slowly towards the cathode.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- BIER M., DUKE J. A., GIBBS R. J., NORD F. F. Composition and fractionation of ovomucoid. Arch Biochem Biophys. 1952 Jun;37(2):491–493. doi: 10.1016/0003-9861(52)90211-7. [DOI] [PubMed] [Google Scholar]
- BLACKBURN S., LOWTHER A. G. The separation of N-2:4-dinitrophenly amino-acids on paper chromatograms. Biochem J. 1951 Jan;48(1):126–128. doi: 10.1042/bj0480126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FEENEY R. E., STEVENS F. C., OSUGA D. T. The specificities of chicken ovomucoid and ovoinhibitor. J Biol Chem. 1963 Apr;238:1415–1418. [PubMed] [Google Scholar]
- FRAENKEL-CONRAT H., PORTER R. R. The terminal amino groups of conalbumin, ovomucoid and avidin. Biochim Biophys Acta. 1952 Nov;9(5):557–562. [PubMed] [Google Scholar]
- FREDERICQ E., DEUTSCH H. F. Studies on ovomucoid. J Biol Chem. 1949 Dec;181(2):499–510. [PubMed] [Google Scholar]
- GORDON A. H., GROSS J., O'CONNOR D., PITT-RIVERS R. Nature of the circulating thyroid hormone-plasma protein complex. Nature. 1952 Jan 5;169(4288):19–20. doi: 10.1038/169019a0. [DOI] [PubMed] [Google Scholar]
- GOTTSCHALK A., ADA G. L. The separation and quantitative determination of the component sugars of mucoproteins. Biochem J. 1956 Apr;62(4):681–686. doi: 10.1042/bj0620681. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodwin T. W., Morton R. A. The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochem J. 1946;40(5-6):628–632. doi: 10.1042/bj0400628. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JEVONS F. R. The heterogeneity of ovomucoid. Biochim Biophys Acta. 1960 Dec 4;45:384–386. doi: 10.1016/0006-3002(60)91465-7. [DOI] [PubMed] [Google Scholar]
- KETTERER B. A glycoprotein component of hen egg white. Life Sci. 1962 May;1:163–165. doi: 10.1016/0024-3205(62)90012-7. [DOI] [PubMed] [Google Scholar]
- King E. J., Garner R. J. The Colorimetric Determination of Glucose. J Clin Pathol. 1947 Nov;1(1):30–33. doi: 10.1136/jcp.1.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVY A. L. A paper chromatographic method for the quantitative estimation of amino-acids. Nature. 1954 Jul 17;174(4420):126–127. doi: 10.1038/174126a0. [DOI] [PubMed] [Google Scholar]
- LUSH I. E. Genetic polymorphisms in the egg albumen proteins of the domestic fowl. Nature. 1961 Mar 25;189:981–984. doi: 10.1038/189981a0. [DOI] [PubMed] [Google Scholar]
- RHODES M. B., BENNETT N., FEENEY R. E. The flavoprotein-apoprotein system of egg white. J Biol Chem. 1959 Aug;234(8):2054–2060. [PubMed] [Google Scholar]
- STOFFYN P. J., JEANLOZ R. W. Identification of amino sugars by paper chromatography. Arch Biochem Biophys. 1954 Oct;52(2):373–379. doi: 10.1016/0003-9861(54)90137-x. [DOI] [PubMed] [Google Scholar]
- SVENNERHOLM E., SVENNERHOLM L. Quantitative paper partition chromatography of sialic acids. Nature. 1958 Apr 19;181(4616):1154–1155. doi: 10.1038/1811154a0. [DOI] [PubMed] [Google Scholar]
- Sanger F. The free amino groups of insulin. Biochem J. 1945;39(5):507–515. doi: 10.1042/bj0390507. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
- WINZLER R. J. Determination of serum glycoproteins. Methods Biochem Anal. 1955;2:279–311. doi: 10.1002/9780470110188.ch10. [DOI] [PubMed] [Google Scholar]
- WISE R. W., KETTERER B., HANSEN I. A. PRE-ALBUMINS OF EMBRYONIC CHICK PLASMA. Comp Biochem Physiol. 1964 Jul;12:439–443. doi: 10.1016/0010-406x(64)90072-6. [DOI] [PubMed] [Google Scholar]