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. 1996 Dec;144(4):1437–1444. doi: 10.1093/genetics/144.4.1437

Deletion of the Schizophyllum Commune Aα Locus: The Roles of Aα Y and Z Mating-Type Genes

C I Robertson 1, K A Bartholomew 1, C P Novotny 1, R C Ullrich 1
PMCID: PMC1207696  PMID: 8978032

Abstract

The Aα locus is one of four master regulatory loci that determine mating type and regulate sexual development in Schizophyllum commune. We have made a plasmid containing a URA1 gene disruption of the Aα Y1 gene. Y1 is the sole Aα gene in Aα1 strains. We used the plasmid construction to produce an Aα null (i.e., AαΔ) strain by replacing the genomic Y1 gene with URA1 in an Aα1 strain. To characterize the role of the Aα genes in the regulation of sexual development, we transformed various Aα Y and Z alleles into AαΔ strains and examined the acquired mating types and mating abilities of the transformants. These experiments demonstrate that the Aα Y gene is not essential for fungal viability and growth, that a solitary Z Aα mating-type gene does not itself activate development, that Aβ proteins are sufficient to activate the A developmental pathway in the absence of Aα proteins and confirm that Y and Z genes are the sole determinants of Aα mating type. The data from these experiments support and refine our model of the regulation of A-pathway events by Y and Z proteins.

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Selected References

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  1. Banham A. H., Asante-Owusu R. N., Gottgens B., Thompson S., Kingsnorth C. S., Mellor E., Casselton L. A. An N-Terminal Dimerization Domain Permits Homeodomain Proteins To Choose Compatible Partners and Initiate Sexual Development in the Mushroom Coprinus cinereus. Plant Cell. 1995 Jun;7(6):773–783. doi: 10.1105/tpc.7.6.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dranginis A. M. Binding of yeast a1 and alpha 2 as a heterodimer to the operator DNA of a haploid-specific gene. Nature. 1990 Oct 18;347(6294):682–685. doi: 10.1038/347682a0. [DOI] [PubMed] [Google Scholar]
  3. Froeliger E. H., Ullrich R. C., Novotny C. P. Sequence analysis of the URA1 gene encoding orotidine-5'-monophosphate decarboxylase of Schizophyllum commune. Gene. 1989 Nov 30;83(2):387–393. doi: 10.1016/0378-1119(89)90127-3. [DOI] [PubMed] [Google Scholar]
  4. Gillissen B., Bergemann J., Sandmann C., Schroeer B., Bölker M., Kahmann R. A two-component regulatory system for self/non-self recognition in Ustilago maydis. Cell. 1992 Feb 21;68(4):647–657. doi: 10.1016/0092-8674(92)90141-x. [DOI] [PubMed] [Google Scholar]
  5. Goutte C., Johnson A. D. Recognition of a DNA operator by a dimer composed of two different homeodomain proteins. EMBO J. 1994 Mar 15;13(6):1434–1442. doi: 10.1002/j.1460-2075.1994.tb06397.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hendrick J. P., Hodges P. E., Rosenberg L. E. Survey of amino-terminal proteolytic cleavage sites in mitochondrial precursor proteins: leader peptides cleaved by two matrix proteases share a three-amino acid motif. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4056–4060. doi: 10.1073/pnas.86.11.4056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herskowitz I. A regulatory hierarchy for cell specialization in yeast. Nature. 1989 Dec 14;342(6251):749–757. doi: 10.1038/342749a0. [DOI] [PubMed] [Google Scholar]
  8. Isaya G., Sakati W. R., Rollins R. A., Shen G. P., Hanson L. C., Ullrich R. C., Novotny C. P. Mammalian mitochondrial intermediate peptidase: structure/function analysis of a new homologue from Schizophyllum commune and relationship to thimet oligopeptidases. Genomics. 1995 Aug 10;28(3):450–461. doi: 10.1006/geno.1995.1174. [DOI] [PubMed] [Google Scholar]
  9. Keleher C. A., Passmore S., Johnson A. D. Yeast repressor alpha 2 binds to its operator cooperatively with yeast protein Mcm1. Mol Cell Biol. 1989 Nov;9(11):5228–5230. doi: 10.1128/mcb.9.11.5228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koetsier P. A., Schorr J., Doerfler W. A rapid optimized protocol for downward alkaline Southern blotting of DNA. Biotechniques. 1993 Aug;15(2):260–262. [PubMed] [Google Scholar]
  11. Kämper J., Reichmann M., Romeis T., Bölker M., Kahmann R. Multiallelic recognition: nonself-dependent dimerization of the bE and bW homeodomain proteins in Ustilago maydis. Cell. 1995 Apr 7;81(1):73–83. doi: 10.1016/0092-8674(95)90372-0. [DOI] [PubMed] [Google Scholar]
  12. Luo Y., Ullrich R. C., Novotny C. P. Only one of the paired Schizophyllum commune A alpha mating-type, putative homeobox genes encodes a homeodomain essential for A alpha-regulated development. Mol Gen Genet. 1994 Aug 2;244(3):318–324. doi: 10.1007/BF00285460. [DOI] [PubMed] [Google Scholar]
  13. Magae Y., Novotny C., Ullrich R. Interaction of the A alpha Y and Z mating-type homeodomain proteins of Schizophyllum commune detected by the two-hybrid system. Biochem Biophys Res Commun. 1995 Jun 26;211(3):1071–1076. doi: 10.1006/bbrc.1995.1920. [DOI] [PubMed] [Google Scholar]
  14. Marion A. L., Bartholomew K. A., Wu J., Yang H., Novotny C. P., Ullrich R. C. The A alpha mating-type locus of Schizophyllum commune: structure and function of gene X. Curr Genet. 1996 Jan;29(2):143–149. doi: 10.1007/BF02221578. [DOI] [PubMed] [Google Scholar]
  15. Muñoz-Rivas A. M., Specht C. A., Ullrich R. C., Novotny C. P. Isolation of the DNA sequence coding indole-3-glycerol phosphate synthetase and phosphoribosylanthranilate isomerase of Schizophyllum commune. Curr Genet. 1986;10(12):909–913. doi: 10.1007/BF00398288. [DOI] [PubMed] [Google Scholar]
  16. Papazian H P. The Incompatibility Factors and a Related Gene in Schizophyllum Commune. Genetics. 1951 Sep;36(5):441–459. doi: 10.1093/genetics/36.5.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]
  18. Shen G. P., Park D. C., Ullrich R. C., Novotny C. P. Cloning and characterization of a Schizophyllum gene with A beta 6 mating-type activity. Curr Genet. 1996 Jan;29(2):136–142. doi: 10.1007/BF02221577. [DOI] [PubMed] [Google Scholar]
  19. Specht C. A., DiRusso C. C., Novotny C. P., Ullrich R. C. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem. 1982 Jan 1;119(1):158–163. doi: 10.1016/0003-2697(82)90680-7. [DOI] [PubMed] [Google Scholar]
  20. Specht C. A., Stankis M. M., Giasson L., Novotny C. P., Ullrich R. C. Functional analysis of the homeodomain-related proteins of the A alpha locus of Schizophyllum commune. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7174–7178. doi: 10.1073/pnas.89.15.7174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Stankis M. M., Specht C. A., Yang H., Giasson L., Ullrich R. C., Novotny C. P. The A alpha mating locus of Schizophyllum commune encodes two dissimilar multiallelic homeodomain proteins. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7169–7173. doi: 10.1073/pnas.89.15.7169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

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