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. 1996 Dec;144(4):1601–1610. doi: 10.1093/genetics/144.4.1601

P-Element-Induced Recombination in Drosophila Melanogaster: Hybrid Element Insertion

YHM Gray 1, M M Tanaka 1, J A Sved 1
PMCID: PMC1207712  PMID: 8978048

Abstract

It has previously been shown that the combination of two deleted P elements in trans, one containing the left functional end and the second element the right functional end, can lead to high levels of male recombination. This finding strongly suggests that P-element ends from different chromosomes can become associated, followed by ``pseudo-excision.'' We show that two different processes are involved in resolving the pseudo-excision event: (1) the excised P-element ends continue to function as a single unit (Hybrid Element) and insert at a nearby site in the chromosome or into the element itself [Hybrid Element Insertion (HEI)] and (2) free ends that do not contain P elements repair and rejoin [(Hybrid Excision and Repair (HER)]. Both types of resolution can lead to recombination, and this paper concentrates on the HEI class. One type of HEI event predicts the exact reverse complementary duplication of an 8-bp target site, and we have confirmed the existence of such a structure in six independently derived recombinant chromosomes. There is also a high tendency for insertion events to occur within a few bases of the original 8-bp target site, including six apparent cases of insertion into the exact site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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