Skip to main content
PMC home page
Genetics logoLink to Genetics
. 1977 May;86(1):133–148. doi: 10.1093/genetics/86.1.133

Sites of the 5s Ribosomal Genes in Drosophila. I. the Multiple Clusters in the VIRILIS Group

D E Wimber 1, Doris R Wimber 1
PMCID: PMC1213660  PMID: 885339

Abstract

Drosophila melanogaster 125I-5S RNA was annealed to salivary gland preparations of 6 species in the virilis group of Drosophila. Two patterns of annealing were found. D. virilis, D. montana and D. borealis showed three 5S gene clusters on chromosome 5; Sd–f and Wc–j were strongly labeled, but Xa–e was weakly labeled. D. montana and D. borealis have a greater percentage of their total 5S cistrons at S d–f than does D. virilis. D. americana americana, D. americana texana and D. novamexicana showed 2 sites labeled; no label was seen at Sd–f while Wc–j was weakly labeled and Xa–e was strongly labeled, the reciprocal of the previous pattern in the W-X region. Hybrids between D. a. americana and D. virilis showed no difference in chromosome banding at the sites of the 5S clusters despite their pattern differences. D. a texana x D. virilis, on the other hand, did show a difference in staining the Xa–e region. These patterns fall squarely into the biosystematic groupings deduced by many previous workers.

Full Text

The Full Text of this article is available as a PDF (5.9 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alonso C., Berendes H. D. The location of 5S (ribosomal) RNA genes in Drosophila hydei. Chromosoma. 1975 Aug 11;51(4):347–356. doi: 10.1007/BF00326321. [DOI] [PubMed] [Google Scholar]
  2. Brown D. D., Sugimoto K. 5 S DNAs of Xenopus laevis and Xenopus mulleri: evolution of a gene family. J Mol Biol. 1973 Aug 15;78(3):397–415. doi: 10.1016/0022-2836(73)90464-6. [DOI] [PubMed] [Google Scholar]
  3. Brownlee G. G., Cartwright E. M., Brown D. D. Sequence studies of the 5 S DNA of Xenopus laevis. J Mol Biol. 1974 Nov 15;89(4):703–718. doi: 10.1016/0022-2836(74)90046-1. [DOI] [PubMed] [Google Scholar]
  4. Cohen M., Jr Ectopic pairing and evolution of 5S ribosomal RNA genes in the chromosomes of Drosophila funebris. Chromosoma. 1976 Jun 23;55(4):349–357. doi: 10.1007/BF00292830. [DOI] [PubMed] [Google Scholar]
  5. Hsu T. C., Spirito S. E., Pardue M. L. Distribution of 18+28S ribosomal genes in mammalian genomes. Chromosoma. 1975 Nov 20;53(1):25–36. doi: 10.1007/BF00329388. [DOI] [PubMed] [Google Scholar]
  6. Hughes R. D. An Analysis of the Chromosomes of the Two Sub-Species Drosophila Virilis Virilis and Drosophila Virilis Americana. Genetics. 1939 Nov;24(6):811–834. doi: 10.1093/genetics/24.6.811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hutchison N., Pardue M. L. The mitotic chromosomes of Notophthalmus (=Triturus) viridescens: localization of C banding regions and DNA sequences complementary to 18S, 28S and 5S ribosomal RNA. Chromosoma. 1975 Nov 20;53(1):51–69. doi: 10.1007/BF00329390. [DOI] [PubMed] [Google Scholar]
  8. Stone W. S., Guest W. C., Wilson F. D. THE EVOLUTIONARY IMPLICATIONS OF THE CYTOLOGICAL POLYMORPHISM AND PHYLOGENY OF THE VIRILIS GROUP OF DROSOPHILA. Proc Natl Acad Sci U S A. 1960 Mar;46(3):350–361. doi: 10.1073/pnas.46.3.350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES