Abstract
The mutator gene DEL1 in the yeast Saccharomyces cerevisiae causes a high rate of formation of multisite mutations that encompass the following three adjacent genes: CYC1, which determines the structure of iso-1-cytochrome c; RAD7, which controls UV sensitivity; and OSM1, which controls osomotic sensitivity. The simplest hypothesis is that these multisite mutations are deletions, although it has not been excluded that they may involve other types of gross chromosomal aberrations. In contrast, normal strains do not produce such multisite mutations even after mutagenic treatments.—The multisite mutations arise at a rate of approximately 10-5 to 10-6 per cell per division in DEL1 strains, which is much higher than rates observed for mutation of genes in normal strains. For example, normal strains produce all types of cyc1 mutants at a low rate of approximately 10-8 to 10-9. No evidence for multisite mutations was obtained upon analysis of numerous spontaneous ade1, ade2, met2 and met15 mutants isolated in a DEL1 strain. DEL1 segregates as a single Mendelian gene closely linked to the CYC1 locus. DEL1 appears to be both cis- and trans-dominant. The location of the DEL1 gene and the lack of effect on other genes suggest that the mutator acts only on a region adjacent to itself.
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Selected References
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- Gilmore R. A. Super-suppressors in Saccharomyces cerevisiae. Genetics. 1967 Aug;56(4):641–658. doi: 10.1093/genetics/56.4.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
- Schweingruber M. E., Sherman F., Stewart J. W. Altered absorption spectra of iso-1-cytochromes c from mutants of yeast. J Biol Chem. 1977 Oct 10;252(19):6577–6580. [PubMed] [Google Scholar]
- Sherman F., Jackson M., Liebman S. W., Schweingruber A. M., Stewart J. W. A deletion map of cyc1 mutants and its correspondence to mutationally altered iso-1-cytochromes c of yeast. Genetics. 1975 Sep;81(1):51–73. doi: 10.1093/genetics/81.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherman F., Stewart J. W., Jackson M., Gilmore R. A., Parker J. H. Mutants of yeast defective in iso-1-cytochrome c. Genetics. 1974 Jun;77(2):255–284. doi: 10.1093/genetics/77.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singh A., Sherman F. Characteristics and relationships of mercury-resistant mutants and methionine auxotrophs of yeast. J Bacteriol. 1974 Jun;118(3):911–918. doi: 10.1128/jb.118.3.911-918.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]