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. 1965 May;95(2):301–310. doi: 10.1042/bj0950301

Movements of radioactive sodium in cerebral-cortex slices in response to electrical stimulation

J C Keesey 1, H Wallgren 1
PMCID: PMC1214324  PMID: 14340078

Abstract

1. Sodium exchange was measured with 24Na in incubated guinea-pig cerebral-cortex slices maintained under adequate metabolic conditions with a steady content of fluid and ions resembling that of brain in vivo. 2. Evidence was obtained indicating that Na+ ions behaved in the inulin space as if they were extracellular, and that their entry into the non-inulin space of unstimulated tissue was about 10 times slower and could be separated, on the basis of complete exchangeability, into two components, a `fast' one, which reacted to electrical stimulation, and a `slow' one, exchanging at a rate of about 8μequiv./g./hr., which was not affected by stimulation. 3. The average rate of sodium turnover in unstimulated slices was 175–275μequiv./g./hr., whereas that for stimulated slices was approx. 4–6 times this, or 1050–1180μequiv./g./hr. The stimulated rate was equivalent to a turnover of 32% of the sodium in the non-inulin space/min., or 3mμequiv./g./impulse. 4. Response to the onset of stimulation appeared to be immediate, but after cessation of stimulation increased sodium movements persisted for several minutes before return to unstimulated values. 5. Calculations based on electrochemical gradients suggested that about one-quarter of the energy available from respiration was required for sodium and potassium transport at maximal rates in both unstimulated and stimulated cerebral-cortex slices.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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