Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1965 May;95(2):490–496. doi: 10.1042/bj0950490

The adenosine-triphosphatase activity of adrenal chromaffin granules

P Banks 1,*
PMCID: PMC1214348  PMID: 14340099

Abstract

1. The preparation of a fraction containing highly purified chromaffin granules from the bovine adrenal medulla is described. 2. The fraction contains an adenosine-triphosphatase activity that is stimulated by Mg2+ and that cannot be explained by contamination with mitochondria or microsomes. 3. It is suggested that the adenosine-triphosphatase activity is related to the uptake of cate-cholamines by the chromaffin granules.

Full text

PDF
491

Images in this article

494-1PLATE 1
on p.494-1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLASCHKO H., BORN G. V., D'IORIO A., EADE N. R. Observations on the distribution of catechol amines and adenosinetriphosphate in the bovine adrenal medulla. J Physiol. 1956 Sep 27;133(3):548–557. doi: 10.1113/jphysiol.1956.sp005607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLASCHKO H., HAGEN J. M., HAGEN P. Mitochondrial enzymes and chromaffin granules. J Physiol. 1957 Dec 3;139(2):316–322. doi: 10.1113/jphysiol.1957.sp005893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARLSSON A., HILLARP N. A., HOKFELT B. The concomitant release of adenosine triphosphate and catechol amines from the adrenal medulla. J Biol Chem. 1957 Jul;227(1):243–252. [PubMed] [Google Scholar]
  4. CARLSSON A., HILLARP N. A., WALDECK B. A Mg-ATP dependent storage mechanism in the amine granules of the adrenal medulla. Med Exp Int J Exp Med. 1962;6:47–53. [PubMed] [Google Scholar]
  5. DOUGLAS W. W., RUBIN R. P. The role of calcium in the secretory response of the adrenal medulla to acetylcholine. J Physiol. 1961 Nov;159:40–57. doi: 10.1113/jphysiol.1961.sp006791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FORTIER A., LEDUC J., D'IORIO A. Biochemical composition of the chromaffin granules of the medulla. Rev Can Biol. 1959 Jun;18(2):110–114. [PubMed] [Google Scholar]
  7. HILLARP N. A. Enzymic systems involving adenosinephosphates in the adrenaline and noradrenaline containing granules of the adrenal medulla. Acta Physiol Scand. 1958 Feb 4;42(2):144–165. doi: 10.1111/j.1748-1716.1958.tb01548.x. [DOI] [PubMed] [Google Scholar]
  8. HILLARP N. A. Isolation and some biochemical properties of the catechol amine granules in the cow adrenal medulla. Acta Physiol Scand. 1958 Jul 17;43(1):82–96. doi: 10.1111/j.1748-1716.1958.tb01579.x. [DOI] [PubMed] [Google Scholar]
  9. KIRSHNER N. Uptake of catecholamines by a particulate fraction of the adrenal medulla. Science. 1962 Jan 12;135(3498):107–108. doi: 10.1126/science.135.3498.107. [DOI] [PubMed] [Google Scholar]
  10. KIRSHNER N. Uptake of catecholamines by a particulate fraction of the adrenal medulla. J Biol Chem. 1962 Jul;237:2311–2317. [PubMed] [Google Scholar]
  11. PHILIPPU A., SCHUEMANN H. J. [The influence of calcium on catecholamine liberation]. Experientia. 1962 Mar 15;18:138–140. doi: 10.1007/BF02153862. [DOI] [PubMed] [Google Scholar]
  12. SCHUMANN H. J. Die Wirkung von Insulin und Reserpin auf den Adrenalin- und ATP-Gehalt der chromaffinen Granula des Nebennierenmarks. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1958;233(3):237–249. [PubMed] [Google Scholar]
  13. SCHUMANN H. J. The distribution of adrenaline and noradrenaline in chromaffin granules from the chicken. J Physiol. 1957 Jul 11;137(2):318–326. doi: 10.1113/jphysiol.1957.sp005815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. STREHLER B. L., TOTTER J. R. Determination of ATP and related compounds: firefly luminescence and other methods. Methods Biochem Anal. 1954;1:341–356. doi: 10.1002/9780470110171.ch13. [DOI] [PubMed] [Google Scholar]
  15. UDENFRIEND S., COOPER J. R., CLARK C. T., BAER J. E. Rate of turnover of epinephrine in the adrenal medulla. Science. 1953 Jun 12;117(3050):663–665. doi: 10.1126/science.117.3050.663. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES