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. 1992 Jan;119(1):49–54. doi: 10.1007/BF01209488

Argyrophilic nucleolar organizer regions in human adrenocortical neoplasms

Yoshiki Kida 1,✉, Yasuo Takano 1, Masahiko Okudaira 1
PMCID: PMC12201051  PMID: 1400566

Abstract

Argyrophilic nucleolar organizer regions (AgNOR) in human adrenocortical neoplasms, including five carcinomas and ten adenomas, were studied using a semi-automatic image analyzer. Both the number and total area of AgNOR per nucleus in the carcinomas were found to be statistically greater than in adenomas and control tissues. However, there were no statistically significant differences in total AgNOR area per nuclear area or in the mean area of individual AgNOR dots. The AgNOR of neoplastic and normal cells were of four morphological types: type 1 had a few dots at the periphery of the nucleus, type 2 a few dots at the center, type 3 a large round dot along with several small ones at the center, and type 4 numerous diffusely distributed polymorphic dots. Most type 3 and 4 cells were found in carcinoma cases. Type 1 cells decreased in proportion to the severity of biological malignancy. It follows from these findings that careful observation of AgNOR should facilitate the distinction of malignant from benign adrenocortical neoplasms.

Key words: Adrenocortical neoplasms, Nucleolar organizer regions, Image analysis, Morphology

Abbreviation

AgNOR

argyrophilic nucleolar organizer region

References

  1. Bowlby LS, DeBault LE, Abraham SR (1986) Flow cytometric analysis of adrenal cortical tumor DNA. Cancer 58: 1499–1505 [DOI] [PubMed] [Google Scholar]
  2. Camuto P, Schinella R, Gilchrist K (1987) Flow cytometric study of adrenal cortical carcinoma, abstract. Lab. Invest 56: 10A [DOI] [PubMed] [Google Scholar]
  3. Chin LS, Hilton DR (1991) The standardized assessment of argyrophilic nucleolar organizer regions in meningeal tumors. J Neurosurg 74: 590–596 [DOI] [PubMed] [Google Scholar]
  4. Cibas ES, Medeiros LJ, Weinberg DS, Gelb AB, Weiss LM (1990) Celluar DNA profiles of benign and malignant adrenocortical tumors. Am J Pathol 14: 948–955 [DOI] [PubMed] [Google Scholar]
  5. Cooper HS (1984) Lectins as probes in histochemistry and immunohistochemistry: the peanut (Arachis hypogaea) lectin. Hum Pathol 15: 904–906 [DOI] [PubMed] [Google Scholar]
  6. Crocker J, Egan MJ (1988) Correlation between NOR size and numbers in non-Hodgkin's lymphomas. J Pathol 156: 233–239 [DOI] [PubMed] [Google Scholar]
  7. Crocker J, Nar P (1987) Nucleolar organizer regions in lymphomas. J Pathol 151: 111–118 [DOI] [PubMed] [Google Scholar]
  8. Crocker J, Boldy DAR, Egan MJ (1989) How should we count AgNORs? Proposals for a standardized approach. J Pathol 158: 185–188 [DOI] [PubMed] [Google Scholar]
  9. Delahunt B, Ribas JL, Nacey JN, Bethwaite PB (1991) Nucleolar organizer regions and prognosis in renal cell carcinoma. J Pathol 163: 31–37 [DOI] [PubMed] [Google Scholar]
  10. Evans HJ, Buckland RA, Pardue ML (1974) Location of the genes coding for 18S and 28S ribosomal RNA in the human genome. Chromosoma 48: 405–426 [Google Scholar]
  11. Hall PA, Levison DA (1990) Review: assessment of cell proliferation in histological material. J Clin Pathol 43: 184–192 [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henderson AS, Warburton D, Atwood KC (1972) Location of ribosomal DNA in the human chromosome complement. Proc Natl Acad Sci 69: 3394–3398 [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hernandez-Verdun D (1983) The nucleolar organizer regions. Biol Cell 49: 191–202 [PubMed] [Google Scholar]
  14. Hough AJ, Hollifield JW, Page DL, Hartmann WH (1979) Prognostic factors in adrenal cortical tumors, a mathematical analysis of clinical and morphological data. Am J Clin Pathol 72: 390–393 [DOI] [PubMed] [Google Scholar]
  15. Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015 [DOI] [PubMed] [Google Scholar]
  16. Howell WM, Denton TE, Diamond JR (1975) Differential staining of the satellite regions of acrocentric chromosome. Experientia 31: 260–262 [DOI] [PubMed] [Google Scholar]
  17. Karakousis CP, Rao U, Moore R (1985) Adrenal adenocarcinomas: histologic grading and survival. J Surg Oncol 29: 105–111 [DOI] [PubMed] [Google Scholar]
  18. Klein FA, Kay S, Ratliff JE (1985) Flow cytometric determination of ploidity and proliferation patterns of adrenal neoplasms: An adjunct to histological classification. J Urol 134: 862–866 [DOI] [PubMed] [Google Scholar]
  19. Miettinen M, Lehto VP, Virtanen I (1985) Immunofluorescence microscopic evaluation of the adrenal cortex and medulla and their tumors. Am J Pathol 118: 360–366 [PMC free article] [PubMed] [Google Scholar]
  20. Nairn ER, Crocker J, McGovern J (1988) Limited value of AgNORs enumeration in assessment of thyroid neoplasms. J Clin Pathol 41: 1136 [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ochs RL, Busch H, (1984) Further evidence that phosphoprotein C23 (110 kD/pI 5.1) is the nucleolar silver staining protein. Exp Cell Res 152: 260–265 [DOI] [PubMed] [Google Scholar]
  22. Orrell JM, Evanse AT, Grant A (1991) A critical evaluation of AgNOR counting in benign naevi and malignant melanoma. J Pathol 163: 239–244 [DOI] [PubMed] [Google Scholar]
  23. Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet JJ (1986) Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at optical level. Histochem J 18: 5–14 [DOI] [PubMed] [Google Scholar]
  24. Quinn CM, Wright NA (1990) The clinical assessment of methods and applications as prognostic variables. J Pathol 60: 93–102 [DOI] [PubMed] [Google Scholar]
  25. Ruschoff J, Neumann K, Contractor H, Plate K, Thomas C (1990) Assessment of nucleolar organizer regions by automatic image analysis in breast cancer: correlation with DNA content, proliferation rate, receptor status and histopathological grading. J Cancer Res Clin Oncol 116: 480–485 [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sasano H, Nose M, Sasano N (1989) Lectin histochemistry in adrenocortical hyperplasia and neoplasms with emphasis on carcinoma. Arch Pathol Lab Med 113: 68–72 [PubMed] [Google Scholar]
  27. Sasano H, Saito Y, Sato I (1990) Nucleolar organizer regions in human adrenocortical disorders. Mod Pathol 3: 591–595 [PubMed] [Google Scholar]
  28. Slooten HV, Schaberg A, Smeenk D, Moolennar AJ (1985) Morphologic characteristics of benign and malignant adrenocortical tumors. Cancer 55: 776–773 [DOI] [PubMed] [Google Scholar]
  29. Soomro I, Patel N, Whimster WF (1991) Distribution and estimation of nucleolar organizer regions in various human lung tumors. Pathol Res Pract 187: 68–72 [DOI] [PubMed] [Google Scholar]
  30. Trere D, Pession A, Derenzini M (1989) The silver-stained proteins of interphasic nucleolar organizer regions as a parameter of cell duplication rate. Exp Cell Res 184: 131–137 [DOI] [PubMed] [Google Scholar]
  31. Weiss LM, Medeiros LJ, Vickery AL (1989) Pathologic features of prognostic significance in adrenocortical carcinoma. Am J Surg Pathol 13: 202–206 [DOI] [PubMed] [Google Scholar]
  32. Wick MR, Cherwitz DL, McGlennen RC, Dehner LP (1986) Adrenocortical carcinoma, an immunohistochemical comparison with renal cell carcinoma. Am J Pathol 122: 343–352 [PMC free article] [PubMed] [Google Scholar]
  33. Williams MA, Kleinschmidt JA, Krohne G, Franke WW (1982) Argyrophilic nuclear and nucleolar proteins ofXenopus laevis oocytes identified by gel electrophoresis. Exp Cell Res 137: 341–351 [DOI] [PubMed] [Google Scholar]
  34. Wrba F, Augustin I, Fertl H (1991) Nucleolar organizer regions in soft tissue sarcomas. Oncology 48: 166–170 [DOI] [PubMed] [Google Scholar]

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