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. 1995 Mar;121(3):169–174. doi: 10.1007/BF01198099

The effect of a mesogenic and a lentogenic Newcastle disease virus strain on Burkitt lymphoma Daudi cells

Y Tzadok-David 1, M Metzkin-Eizenberg 1, Z Zakay-Rones 1,✉
PMCID: PMC12201484  PMID: 7713989

Abstract

The destructive effect of Newcastle disease virus (NDV) strains on Burkitt lymphoma Daudi cells was investigated. Interaction of an active and UV-inactivated mesogenic strain (Roakin), as well as an active attenuated lentogenic strain (B1), grown in the allantoic sac of embryonated eggs, at high multiplicity, caused inhibition in cellular DNA synthesis and arrest in cell multiplication, eventually killing of the cells. The lentogenic strain cultivated in chicken fibroblasts exhibited only a moderate activity. The mechanism of the cytolytic effect is presumably linked to the increase in cell membrane permeability indicated by the elevation in51Cr release. Thus it appears that the massive adsorption and/or penetration of viral particles, active or UV-inactivated (or possibly a toxic component that resides in the virion), damages the plasma membrane and may be responsible for the killing of the cells.

Key words: Daudi Burkitt lymphoma cells, Newcastle disease virus, Viral cytotoxicity, UV-inactivated virus

Abbreviations

CEF

chicken embryo fibroblasts

NDV

Newcastle disease virus

B1

attenuated lentogenic NDV strain B1 V-188

RO

mesogenic (medium virulent) NDV strain Roakin/46 V-109 NJ

CEF-B1

B1 cultivated in CEF

RO-IRR

UV-irradiated RO

m.o.i.

multiplicity of infection

EID50

median egg-infective dose

PBS

phosphate-buffered saline

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