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. 2000 Jan 15;345(Pt 2):181–184.

Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action.

Q X Zhang 1, C S Pilquil 1, J Dewald 1, L G Berthiaume 1, D N Brindley 1
PMCID: PMC1220744  PMID: 10620492

Abstract

Lipid phosphate phosphatase-1 (LPP-1) dephosphorylates exogenous lysophosphatidate and thereby regulates the activation of lysophosphatidate receptors and cell division. Mutation of seven amino acids in three conserved domains of mouse LPP-1 abolished its activity. A glycosylation site was demonstrated between conserved Domains 1 and 2. LPP-1 is expressed in the plasma membrane, and the present results demonstrate the active site to be located on the outer surface.

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Selected References

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  1. An S., Bleu T., Hallmark O. G., Goetzl E. J. Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid. J Biol Chem. 1998 Apr 3;273(14):7906–7910. doi: 10.1074/jbc.273.14.7906. [DOI] [PubMed] [Google Scholar]
  2. Andersson S., Davis D. L., Dahlbäck H., Jörnvall H., Russell D. W. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. J Biol Chem. 1989 May 15;264(14):8222–8229. [PubMed] [Google Scholar]
  3. Barilà D., Plateroti M., Nobili F., Muda A. O., Xie Y., Morimoto T., Perozzi G. The Dri 42 gene, whose expression is up-regulated during epithelial differentiation, encodes a novel endoplasmic reticulum resident transmembrane protein. J Biol Chem. 1996 Nov 22;271(47):29928–29936. doi: 10.1074/jbc.271.47.29928. [DOI] [PubMed] [Google Scholar]
  4. Brindley D. N., Waggoner D. W. Mammalian lipid phosphate phosphohydrolases. J Biol Chem. 1998 Sep 18;273(38):24281–24284. doi: 10.1074/jbc.273.38.24281. [DOI] [PubMed] [Google Scholar]
  5. Fukushima N., Kimura Y., Chun J. A single receptor encoded by vzg-1/lpA1/edg-2 couples to G proteins and mediates multiple cellular responses to lysophosphatidic acid. Proc Natl Acad Sci U S A. 1998 May 26;95(11):6151–6156. doi: 10.1073/pnas.95.11.6151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guo Z., Liliom K., Fischer D. J., Bathurst I. C., Tomei L. D., Kiefer M. C., Tigyi G. Molecular cloning of a high-affinity receptor for the growth factor-like lipid mediator lysophosphatidic acid from Xenopus oocytes. Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14367–14372. doi: 10.1073/pnas.93.25.14367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hemrika W., Renirie R., Dekker H. L., Barnett P., Wever R. From phosphatases to vanadium peroxidases: a similar architecture of the active site. Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2145–2149. doi: 10.1073/pnas.94.6.2145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hemrika W., Wever R. A new model for the membrane topology of glucose-6-phosphatase: the enzyme involved in von Gierke disease. FEBS Lett. 1997 Jun 16;409(3):317–319. doi: 10.1016/s0014-5793(97)00530-9. [DOI] [PubMed] [Google Scholar]
  9. Ho S. N., Hunt H. D., Horton R. M., Pullen J. K., Pease L. R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 1989 Apr 15;77(1):51–59. doi: 10.1016/0378-1119(89)90358-2. [DOI] [PubMed] [Google Scholar]
  10. Hooks S. B., Ragan S. P., Lynch K. R. Identification of a novel human phosphatidic acid phosphatase type 2 isoform. FEBS Lett. 1998 May 8;427(2):188–192. doi: 10.1016/s0014-5793(98)00421-9. [DOI] [PubMed] [Google Scholar]
  11. Jasinska R., Zhang Q. X., Pilquil C., Singh I., Xu J., Dewald J., Dillon D. A., Berthiaume L. G., Carman G. M., Waggoner D. W. Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters. Biochem J. 1999 Jun 15;340(Pt 3):677–686. [PMC free article] [PubMed] [Google Scholar]
  12. Kai M., Wada I., Imai S. i., Sakane F., Kanoh H. Cloning and characterization of two human isozymes of Mg2+-independent phosphatidic acid phosphatase. J Biol Chem. 1997 Sep 26;272(39):24572–24578. doi: 10.1074/jbc.272.39.24572. [DOI] [PubMed] [Google Scholar]
  13. Kai M., Wada I., Imai S., Sakane F., Kanoh H. Identification and cDNA cloning of 35-kDa phosphatidic acid phosphatase (type 2) bound to plasma membranes. Polymerase chain reaction amplification of mouse H2O2-inducible hic53 clone yielded the cDNA encoding phosphatidic acid phosphatase. J Biol Chem. 1996 Aug 2;271(31):18931–18938. doi: 10.1074/jbc.271.31.18931. [DOI] [PubMed] [Google Scholar]
  14. Lei K. J., Pan C. J., Liu J. L., Shelly L. L., Chou J. Y. Structure-function analysis of human glucose-6-phosphatase, the enzyme deficient in glycogen storage disease type 1a. J Biol Chem. 1995 May 19;270(20):11882–11886. doi: 10.1074/jbc.270.20.11882. [DOI] [PubMed] [Google Scholar]
  15. Messerschmidt A., Prade L., Wever R. Implications for the catalytic mechanism of the vanadium-containing enzyme chloroperoxidase from the fungus Curvularia inaequalis by X-ray structures of the native and peroxide form. Biol Chem. 1997 Mar-Apr;378(3-4):309–315. doi: 10.1515/bchm.1997.378.3-4.309. [DOI] [PubMed] [Google Scholar]
  16. Messerschmidt A., Wever R. X-ray structure of a vanadium-containing enzyme: chloroperoxidase from the fungus Curvularia inaequalis. Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):392–396. doi: 10.1073/pnas.93.1.392. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Moolenaar W. H. Lysophosphatidic acid, a multifunctional phospholipid messenger. J Biol Chem. 1995 Jun 2;270(22):12949–12952. doi: 10.1074/jbc.270.22.12949. [DOI] [PubMed] [Google Scholar]
  18. Neuwald A. F. An unexpected structural relationship between integral membrane phosphatases and soluble haloperoxidases. Protein Sci. 1997 Aug;6(8):1764–1767. doi: 10.1002/pro.5560060817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pan C. J., Lei K. J., Annabi B., Hemrika W., Chou J. Y. Transmembrane topology of glucose-6-phosphatase. J Biol Chem. 1998 Mar 13;273(11):6144–6148. doi: 10.1074/jbc.273.11.6144. [DOI] [PubMed] [Google Scholar]
  20. Roberts R., Sciorra V. A., Morris A. J. Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform. J Biol Chem. 1998 Aug 21;273(34):22059–22067. doi: 10.1074/jbc.273.34.22059. [DOI] [PubMed] [Google Scholar]
  21. Stukey J., Carman G. M. Identification of a novel phosphatase sequence motif. Protein Sci. 1997 Feb;6(2):469–472. doi: 10.1002/pro.5560060226. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Waggoner D. W., Gómez-Muñoz A., Dewald J., Brindley D. N. Phosphatidate phosphohydrolase catalyzes the hydrolysis of ceramide 1-phosphate, lysophosphatidate, and sphingosine 1-phosphate. J Biol Chem. 1996 Jul 12;271(28):16506–16509. doi: 10.1074/jbc.271.28.16506. [DOI] [PubMed] [Google Scholar]
  23. Waggoner D. W., Martin A., Dewald J., Gómez-Muñoz A., Brindley D. N. Purification and characterization of novel plasma membrane phosphatidate phosphohydrolase from rat liver. J Biol Chem. 1995 Aug 18;270(33):19422–19429. doi: 10.1074/jbc.270.33.19422. [DOI] [PubMed] [Google Scholar]
  24. Waggoner D. W., Xu J., Singh I., Jasinska R., Zhang Q. X., Brindley D. N. Structural organization of mammalian lipid phosphate phosphatases: implications for signal transduction. Biochim Biophys Acta. 1999 Jul 30;1439(2):299–316. doi: 10.1016/s1388-1981(99)00102-x. [DOI] [PubMed] [Google Scholar]

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