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. 2000 Oct 1;351(Pt 1):251–256. doi: 10.1042/0264-6021:3510251

Activity of the human cytochrome c1 promoter is modulated by E2F.

K Luciakova 1, P Barath 1, R Li 1, A Zaid 1, B D Nelson 1
PMCID: PMC1221356  PMID: 10998368

Abstract

The human cytochrome c(1) promoter is strongly activated in transfected Drosophila SL2 cells expressing exogenous human E2F1. Transfection-deletion experiments, DNase I protection by E2F1 and gel mobility-shift experiments locate E2F1 activation sites to two regions on either side of the transcription start site. Deletion of either region prevents E2F1 activation in transfected SL2 cells, suggesting a co-operative interaction between them. E2F6, a member of the E2F family that lacks transactivation domains but contains specific suppressor domains, inhibits cytochrome c(1) promoter activity when co-transfected into HeLa cells, indicating that the E2F proteins modulate the cytochrome c(1) promoter in mammalian cells. However, E2F is not a general regulator of oxidative phosphorylation genes since three additional nuclear-encoded mitochondrial genes were unaffected by E2F1 or E2F6.

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Selected References

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