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. 2001 Aug 1;357(Pt 3):779–785. doi: 10.1042/0264-6021:3570779

Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries.

D Patel 1, S Vitovski 1, H J Senior 1, M D Edge 1, R C Hockney 1, M J Dempsey 1, J R Sayers 1
PMCID: PMC1222007  PMID: 11463348

Abstract

A new method for continuous biopanning has been developed. We have combined the power of affinity chromatography with the fecundity of bacteria in a unique process that mimics clonal selection. Mixed populations of bacteria were applied to a fermenter containing the immobilized ligand of interest. Bacteria retained in this affinity fermenter were allowed to grow under continuous washout conditions, such that weakly bound organisms were selectively lost. Those initially rare founder bacteria expressing a receptor for the immobilized ligand (R+ve) were thus enriched and amplified simultaneously. From an initial culture containing 1 x 10(10) R-ve cells spiked with fewer than 30 R+ve bacteria (<1 in 10(8)), final ratios of R+ve/R-ve bacteria as high as 1 in 12 were observed, representing an enrichment factor of 55 million-fold. This technology has considerable potential for rapid screening of bacterial surface-display libraries and in facilitating directed-evolution studies.

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Selected References

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