Skip to main content
PMC home page
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1983 Jan;47(1):48–53.

A model aerosol exposure system for induction of porcine Haemophilus pleuropneumonia.

T N Sebunya, J R Saunders, A D Osborne
PMCID: PMC1235883  PMID: 6403208

Abstract

One group of six pigs and another group of three pigs were separately exposed in a polyethylene enclosed chamber for ten minutes, respectively, to Haemophilus pleuropneumoniae serotype 1 and Bacillus subtilis aerosols generated by an ultrasonic nebulizer. Haemophilus pleuropneumoniae and B. subtilis were deposited throughout the lungs immediately following aerosol exposure. The number of H. pleuropneumoniae and B. subtilis deposited varied within and between lungs in each group. The mean numbers of both organisms deposited in the posterior (caudal and accessory) lobes were significantly greater than those in the anterior (cranial and middle) lobes (P less than 0.001). The four principals that received H. pleuropneumoniae aerosols and the two contact controls developed fatal fibrinous pneumonia which simulated that seen in natural infections. Since this exposure system consistently resulted in clinical disease it has good potential as a model for the study of pathogenesis of the disease and more specifically for the evaluation of vaccines.

Full text

PDF
51

Images in this article

51Fig. 4.
on p.51
51Fig. 5.
on p.51
51Fig. 6.
on p.51
52Fig. 7.
on p.52
52Fig. 8.
on p.52

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COWN W. B., KETHLEY T. W., FINCHER E. L. The critical-orifice liquid impinger as a sampler for bacterial aerosols. Appl Microbiol. 1957 Mar;5(2):119–124. doi: 10.1128/am.5.2.119-124.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dunnill M. S. Some aspects of pulmonary defence. J Pathol. 1979 Aug;128(4):221–236. doi: 10.1002/path.1711280408. [DOI] [PubMed] [Google Scholar]
  3. Greenway J. A. Hemophilus pneumonia in B.C. swine. Can Vet J. 1981 Jan;22(1):20–21. [PMC free article] [PubMed] [Google Scholar]
  4. Jakab G. J., Green G. M. Defect in intracellular killing of Staphylococcus aureus within alveolar macrophages in Sendai virus-infected murine lungs. J Clin Invest. 1976 Jun;57(6):1533–1539. doi: 10.1172/JCI108423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jay S. J., Johanson W. G., Jr, Pierce A. K., Reisch J. S. Determinants of lung bacterial clearance in normal mice. J Clin Invest. 1976 Apr;57(4):811–817. doi: 10.1172/JCI108356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jericho K. W., Langford E. V., Pantekoek J. Recovery of Pasteurella hemolytica from aerosols at differing temperature and humidity. Can J Comp Med. 1977 Apr;41(2):211–214. [PMC free article] [PubMed] [Google Scholar]
  7. Jericho K. W., O'Connell D. C. Deposition in the respiratory tract of cattle of spores of Bacillus subtilis var niger by inhalation and by nasal instillation. Can J Comp Med. 1974 Jul;38(3):260–265. [PMC free article] [PubMed] [Google Scholar]
  8. Little T. W. Haemophilus infection in pigs. Vet Rec. 1970 Oct 3;87(14):399–402. doi: 10.1136/vr.87.14.399. [DOI] [PubMed] [Google Scholar]
  9. Little T. W., Harding J. D. The comparative pathogenicity of two porcine haemophilus species. Vet Rec. 1971 May 22;88(21):540–545. doi: 10.1136/vr.88.21.540. [DOI] [PubMed] [Google Scholar]
  10. MOTTURA G. Penetration of dust particles and sites of dust stores in pneumoconiosis. Br J Ind Med. 1952 Jan;9(1):65–69. doi: 10.1136/oem.9.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mylrea P. J., Fraser G., Macqueen P., Lambourne D. A. Pleuropneumonia in pigs caused by Haemophilus parahaemolyticus. Aust Vet J. 1974 Jun;50(6):255–259. doi: 10.1111/j.1751-0813.1974.tb05292.x. [DOI] [PubMed] [Google Scholar]
  12. Nielsen R. Pleuropneumonia of swine caused by Haemophilus parahaemolyticus. Studies on the protection obtained by vaccination. Nord Vet Med. 1976 Jul-Aug;28(7-8):337–348. [PubMed] [Google Scholar]
  13. SHOPE R. E. PORCINE CONTAGIOUS PLEUROPNEUMONIA. I. EXPERIMENTAL TRANSMISSION, ETIOLOGY, AND PATHOLOGY. J Exp Med. 1964 Mar 1;119:357–368. doi: 10.1084/jem.119.3.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sanford S. E., Josephson G. K. Porcine Haemophilus pleuropneumonia epizootic in southwestern Ontario: clinical, microbiological, pathological and some epidemiological findings. Can J Comp Med. 1981 Jan;45(1):2–7. [PMC free article] [PubMed] [Google Scholar]
  15. Saunders J. R., Sebunya T. N., Osborne A. D. Pulmonary clearance of Bacillus subtilis spores in pigs. Can J Comp Med. 1983 Jan;47(1):43–47. [PMC free article] [PubMed] [Google Scholar]
  16. Schiefer B., Greenfield J. Porcine Hemophilus parahemolyticus pneumonia in Saskatchewan. II. Bacteriological and experimental studies. Can J Comp Med. 1974 Apr;38(2):105–110. [PMC free article] [PubMed] [Google Scholar]
  17. Schiefer B., Moffatt R. E., Greenfield J., Agar J. L., Majka J. A. Porcine Hemophilus parahemolyticus pneumonia in Saskatchewan. I. Natural occurrence and findings. Can J Comp Med. 1974 Apr;38(2):99–104. [PMC free article] [PubMed] [Google Scholar]
  18. WOLFE E. K., Jr Quantitative characterization of aerosols. Bacteriol Rev. 1961 Sep;25:194–202. doi: 10.1128/br.25.3.194-202.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Won W. D., Ross H. Effect of diluent and relative humidity on apparent viability of airborne Pasteurella pestis. Appl Microbiol. 1966 Sep;14(5):742–745. doi: 10.1128/am.14.5.742-745.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES