Skip to main content
Canadian Journal of Comparative Medicine logoLink to Canadian Journal of Comparative Medicine
. 1983 Jul;47(3):336–340.

Detection of Campylobacter fetus in artificial insemination bulls with a transport enrichment medium.

M M Garcia, G M Ruckerbauer, M D Eaglesome, W E Boisclair
PMCID: PMC1235949  PMID: 6357410

Abstract

One hundred and five bulls from an artificial insemination unit were tested for the presence of Campylobacter fetus subspecies venerealis. The method involved the inoculation of preputial samples into a new transport enrichment medium prior to culture and immunofluorescence tests. Seventeen bulls (16%) were found to be either positive or suspected carriers of C. fetus at one or more sampling times. The average age of these 17 bulls was about two years greater than the average age of all the bulls in the unit. A combined treatment of vaccination and dihydrostreptomycin sulfate injection suppressed or eliminated the organism from carrier bulls. The use of transport enrichment medium has increased our capability and effectiveness to monitor the presence of C. fetus in artificial insemination bulls.

Full text

PDF
337

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berg R. L., Firehammer B. D. Effect of interval between booster vaccination and time of breeding on protection against campylobacteriosis (vibriosis) in cattle. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 1):467–471. [PubMed] [Google Scholar]
  2. Bouters R., De Keyser J., Vandeplassche M., Van Aert A., Brone E., Bonte P. Vibrio fetus infection in bulls: curative and preventive vaccination. Br Vet J. 1973 Jan-Feb;129(1):52–57. doi: 10.1016/s0007-1935(17)36588-0. [DOI] [PubMed] [Google Scholar]
  3. Cameron C. M. Antibody response of guinea-pigs and cattle to a Campylobacter fetus oil emulsion vaccine. Onderstepoort J Vet Res. 1982 Sep;49(3):177–179. [PubMed] [Google Scholar]
  4. Clark B. L., Dufty J. H. Isolation of Campylobacter fetus from bulls. Aust Vet J. 1978 May;54(5):262–263. [PubMed] [Google Scholar]
  5. Clark B. L., Dufty J. H., Monsbourgh M. J., Parsonson I. M. Immunisation against bovine vibriosis. Vaccination of bulls against infection with Campylobacter fetus subsp. venerealis. Aust Vet J. 1974 Sep;50(9):407–409. doi: 10.1111/j.1751-0813.1974.tb05347.x. [DOI] [PubMed] [Google Scholar]
  6. Clark B. L., Dufty J. H., Monsbourgh M. J., Parsonson I. M. Studies on veneral transmission of Campylobacter fetus by immunised bulls. Aust Vet J. 1975 Nov;51(11):531–532. doi: 10.1111/j.1751-0813.1975.tb06904.x. [DOI] [PubMed] [Google Scholar]
  7. Clark B. L., Dufty J. H., Monsbourgh M. J. Vaccination of bulls against bovine vibriosis. Aust Vet J. 1968 Nov;44(11):530–530. doi: 10.1111/j.1751-0813.1968.tb09024.x. [DOI] [PubMed] [Google Scholar]
  8. Clark B. L., Dufty J. H. The duration of protection against infection with Campylobacter fetus subsp. venerealis in immunised bulls. Aust Vet J. 1982 May;58(5):220–220. doi: 10.1111/j.1751-0813.1982.tb00680.x. [DOI] [PubMed] [Google Scholar]
  9. Corbeil L. B., Schurig G. G., Bier P. J., Winter A. J. Bovine veneral vibriosis: antigenic variation of the bacterium during infection. Infect Immun. 1975 Feb;11(2):240–244. doi: 10.1128/iai.11.2.240-244.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dufty J. H., McEntee K. Evaluation of some culture media and sampling techniques for the diagnosis of vibriosis in the bull. Aust Vet J. 1969 Apr;45(4):140–144. doi: 10.1111/j.1751-0813.1969.tb01911.x. [DOI] [PubMed] [Google Scholar]
  11. Garcia M. M., Eaglesome M. D., Hawkins C. F., Alexander F. C. Campylobacteriosis in Jamaican cattle. Vet Rec. 1980 Mar 29;106(13):287–288. doi: 10.1136/vr.106.13.287. [DOI] [PubMed] [Google Scholar]
  12. Lein D., Erickson I., Winter A. J., McEntee K. Diagnosis, treatment, and control of vibriosis in an artificial insemination center. J Am Vet Med Assoc. 1968 Dec 15;153(12):1574–1580. [PubMed] [Google Scholar]
  13. MORGAN W. J. The sensitivity of Vibrio fetus to streptomycin and the emergence of resistant mutants. J Gen Microbiol. 1958 Dec;19(3):517–521. doi: 10.1099/00221287-19-3-517. [DOI] [PubMed] [Google Scholar]
  14. Mitchell D. Some effects of experimental Vibrio fetus (venerealis) infection on cattle inoculated with a commercial bacterin. Can J Comp Med. 1968 Jul;32(3):474–479. [PMC free article] [PubMed] [Google Scholar]
  15. PLASTRIDGE W. N., WALKER E. C., WILLIAMS L. F., STULA E. F., KIGGINS E. M. Isolation of Vibrio fetus from bulls. Am J Vet Res. 1957 Jul;18(68):575–578. [PubMed] [Google Scholar]
  16. Ruckerbauer G. M., Malkin K., Mitchell D., Boulanger P. Vibriosis: demonstration of Vibrio fetus and Vibrio bubulus organisms in preputial fluid by immunofluorescence and cultural techniques. Can J Comp Med. 1974 Jul;38(3):321–327. [PMC free article] [PubMed] [Google Scholar]
  17. Tedesco L. F., Errico F., Del Baglivi L. P. Comparison of three sampling methods for the diagnosis of genital vibriosis in the bull. Aust Vet J. 1977 Oct;53(10):470–472. doi: 10.1111/j.1751-0813.1977.tb05463.x. [DOI] [PubMed] [Google Scholar]
  18. WAGNER W. C., DUNN H. O., VANVLECK L. D. INCIDENCE OF VIBRIOSIS IN AN AI STUD. Cornell Vet. 1965 Apr;55:209–220. [PubMed] [Google Scholar]
  19. Winter A. J., Caveney N. T. Evaluation of a transport medium for campylobacter (Vibrio) fetus. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 1):472–474. [PubMed] [Google Scholar]

Articles from Canadian Journal of Comparative Medicine are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES