Abstract
An enzyme-linked immunosorbent assay (ELISA) was adapted to test serum antibody to enterotoxin of Clostridium perfringens type A. The test was evaluated using sheep, calf and guinea pig sera and compared with passive hemagglutination and immunodiffusion tests. The ELISA was found to be more sensitive than the other two tests and was completely free from nonspecific reactions. The method was considered to be technically advantageous and suitable for semiautomated procedures.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Naik H. S., Duncan C. L. Detection of Clostridium perfringens enterotoxin in human fecal samples and anti-enterotoxin in sera. J Clin Microbiol. 1978 Apr;7(4):337–340. doi: 10.1128/jcm.7.4.337-340.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Niilo L., Bainborough A. R. A survey of Clostridium perfringens enterotoxin antibody in human and animal sera in western Canada. Can J Microbiol. 1980 Sep;26(9):1162–1164. doi: 10.1139/m80-192. [DOI] [PubMed] [Google Scholar]
- Niilo L. Enterotoxigenic Clostridium perfringens type A isolated from intestinal contents of cattle, sheep and chickens. Can J Comp Med. 1978 Jul;42(3):357–363. [PMC free article] [PubMed] [Google Scholar]
- Sakaguchi G., Uemura T., Riemann H. P. Simplified method for purification of Clostridium perfringens type A enterotoxin. Appl Microbiol. 1973 Nov;26(5):762–767. doi: 10.1128/am.26.5.762-767.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skjelkvåle R., Uemura T. Detection of enterotoxin in faeces and anti-enterotoxin in serum after Clostridium perfringens food-poisoning. J Appl Bacteriol. 1977 Jun;42(3):355–363. doi: 10.1111/j.1365-2672.1977.tb00703.x. [DOI] [PubMed] [Google Scholar]