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. 1995 Aug;69(2):619–639. doi: 10.1016/S0006-3495(95)79939-8

Determination of three-dimensional low-resolution viral structure from solution x-ray scattering data.

Y Zheng 1, P C Doerschuk 1, J E Johnson 1
PMCID: PMC1236288  PMID: 8527677

Abstract

The capsid is modeled as a region of constant electron density located between inner and outer envelopes that exhibit icosahedral symmetry. For computational purposes the envelopes are represented as truncated sums of weighted icosahedral harmonics. Methods are described for estimating the weights from x-ray solution scattering patterns based on nonlinear least squares, and two examples of the procedure, for viruses with known atomic-resolution structures, are given.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agrawal D. K., Johnson J. E. Assembly of the T = 4 Nudaurelia capensis omega virus capsid protein, post-translational cleavage, and specific encapsidation of its mRNA in a baculovirus expression system. Virology. 1995 Feb 20;207(1):89–97. doi: 10.1006/viro.1995.1054. [DOI] [PubMed] [Google Scholar]
  2. Harrison S. C. Structure of tomato bushy stunt virus. I. The spherically averaged electron density. J Mol Biol. 1969 Jun 28;42(3):457–483. doi: 10.1016/0022-2836(69)90236-8. [DOI] [PubMed] [Google Scholar]
  3. Jack A., Harrison S. C. On the interpretation of small-angle x-ray solution scattering from spherical viruses. J Mol Biol. 1975 Nov 25;99(1):15–25. doi: 10.1016/s0022-2836(75)80155-0. [DOI] [PubMed] [Google Scholar]
  4. Speir J. A., Munshi S., Wang G., Baker T. S., Johnson J. E. Structures of the native and swollen forms of cowpea chlorotic mottle virus determined by X-ray crystallography and cryo-electron microscopy. Structure. 1995 Jan 15;3(1):63–78. doi: 10.1016/s0969-2126(01)00135-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Wang G. J., Porta C., Chen Z. G., Baker T. S., Johnson J. E. Identification of a Fab interaction footprint site on an icosahedral virus by cryoelectron microscopy and X-ray crystallography. Nature. 1992 Jan 16;355(6357):275–278. doi: 10.1038/355275a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Zlotnick A., Reddy V. S., Dasgupta R., Schneemann A., Ray W. J., Jr, Rueckert R. R., Johnson J. E. Capsid assembly in a family of animal viruses primes an autoproteolytic maturation that depends on a single aspartic acid residue. J Biol Chem. 1994 May 6;269(18):13680–13684. [PubMed] [Google Scholar]

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