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. 1993 Aug;218(2):183–188. doi: 10.1097/00000658-199308000-00010

Developmental expression of the neurotensin gene in the rat liver.

B M Evers 1, S Rajaraman 1, D H Chung 1, C M Townsend Jr 1, X Wang 1, K Graves 1, J C Thompson 1
PMCID: PMC1242928  PMID: 8342998

Abstract

OBJECTIVE: This study determined whether the neurotensin gene is expressed during early development of the liver. SUMMARY BACKGROUND DATA: Neurotensin (NT), a gut tridecapeptide localized mainly to the distal small bowel and brain of adults, is an important hormone regulating gut motility, secretion and mucosal growth. Expression of NT peptide and the gene is found in fibrolamellar hepatocarcinomas, a variant of hepatocellular carcinoma, but not in the normal adult liver. METHODS: Northern and in situ hybridization techniques were used to determine expression of the neurotensin gene (NT/N) in the normal developing liver. RESULTS: NT/N is expressed in the fetal and early postnatal rat liver, but expression is repressed in the liver of the adult. In situ hybridization confirms the authors' Northern data and demonstrates a random distribution of NT/N expression in the fetal and 3-day postnatal liver. CONCLUSIONS: The authors conclude from this study that NT/N is expressed during early development of the rat liver with subsequent repression in the adult. NT/N may be reexpressed with malignant transformation of the liver.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson S., Rosell S., Hjelmquist U., Chang D., Folkers K. Inhibition of gastric and intestinal motor activity in dogs by (Gln4) neurotensin. Acta Physiol Scand. 1977 Jun;100(2):231–235. doi: 10.1111/j.1748-1716.1977.tb05941.x. [DOI] [PubMed] [Google Scholar]
  2. Baca I., Feurle G. E., Schwab A., Mittmann U., Knauf W., Lehnert T. Effect of neurotensin on exocrine pancreatic secretion in dogs. Digestion. 1982;23(3):174–183. doi: 10.1159/000198725. [DOI] [PubMed] [Google Scholar]
  3. Bean A. J., Dagerlind A., Hökfelt T., Dobner P. R. Cloning of human neurotensin/neuromedin N genomic sequences and expression in the ventral mesencephalon of schizophrenics and age/sex matched controls. Neuroscience. 1992 Sep;50(2):259–268. doi: 10.1016/0306-4522(92)90421-w. [DOI] [PubMed] [Google Scholar]
  4. Berman M. A., Burnham J. A., Sheahan D. G. Fibrolamellar carcinoma of the liver: an immunohistochemical study of nineteen cases and a review of the literature. Hum Pathol. 1988 Jul;19(7):784–794. doi: 10.1016/s0046-8177(88)80261-2. [DOI] [PubMed] [Google Scholar]
  5. Blackburn A. M., Bryant M. G., Adrian T. E., Bloom S. R. Pancreatic tumours produce neurotensin. J Clin Endocrinol Metab. 1981 Apr;52(4):820–822. doi: 10.1210/jcem-52-4-820. [DOI] [PubMed] [Google Scholar]
  6. Buamah P. K., McArdle P., Bennett M., James O. F., Lendrum R. A pleomorphic hepatocellular carcinoma with biochemical features of fibrolamellar hepatocellular carcinoma. J Surg Oncol. 1986 Jun;32(2):93–95. doi: 10.1002/jso.2930320210. [DOI] [PubMed] [Google Scholar]
  7. Carraway R. E., Mitra S. P., Feurle G. E., Hacki W. H. Presence of neurotensin and neuromedin-N within a common precursor from a human pancreatic neuroendocrine tumor. J Clin Endocrinol Metab. 1988 Jun;66(6):1323–1328. doi: 10.1210/jcem-66-6-1323. [DOI] [PubMed] [Google Scholar]
  8. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  9. Chung D. H., Evers B. M., Shimoda I., Townsend C. M., Jr, Rajaraman S., Thompson J. C. Effect of neurotensin on gut mucosal growth in rats with jejunal and ileal Thiry-Vella fistulas. Gastroenterology. 1992 Oct;103(4):1254–1259. doi: 10.1016/0016-5085(92)91512-3. [DOI] [PubMed] [Google Scholar]
  10. Collier N. A., Weinbren K., Bloom S. R., Lee Y. C., Hodgson H. J., Blumgart L. H. Neurotensin secretion by fibrolamellar carcinoma of the liver. Lancet. 1984 Mar 10;1(8376):538–540. doi: 10.1016/s0140-6736(84)90934-6. [DOI] [PubMed] [Google Scholar]
  11. Cox K. H., DeLeon D. V., Angerer L. M., Angerer R. C. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol. 1984 Feb;101(2):485–502. doi: 10.1016/0012-1606(84)90162-3. [DOI] [PubMed] [Google Scholar]
  12. Danielson P. E., Forss-Petter S., Brow M. A., Calavetta L., Douglass J., Milner R. J., Sutcliffe J. G. p1B15: a cDNA clone of the rat mRNA encoding cyclophilin. DNA. 1988 May;7(4):261–267. doi: 10.1089/dna.1988.7.261. [DOI] [PubMed] [Google Scholar]
  13. Darnell J. E., Jr Variety in the level of gene control in eukaryotic cells. Nature. 1982 Jun 3;297(5865):365–371. doi: 10.1038/297365a0. [DOI] [PubMed] [Google Scholar]
  14. Derman E., Krauter K., Walling L., Weinberger C., Ray M., Darnell J. E., Jr Transcriptional control in the production of liver-specific mRNAs. Cell. 1981 Mar;23(3):731–739. doi: 10.1016/0092-8674(81)90436-0. [DOI] [PubMed] [Google Scholar]
  15. Dobner P. R., Barber D. L., Villa-Komaroff L., McKiernan C. Cloning and sequence analysis of cDNA for the canine neurotensin/neuromedin N precursor. Proc Natl Acad Sci U S A. 1987 May;84(10):3516–3520. doi: 10.1073/pnas.84.10.3516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Evers B. M., Beauchamp R. D., Ishizuka J., Townsend C. M., Jr, Alam T., Papaconstantinou J., Thompson J. C. Posttranscriptional regulation of neurotensin in the gut. Surgery. 1991 Aug;110(2):247–252. [PubMed] [Google Scholar]
  17. Evers B. M., Ishizuka J., Chung D. H., Townsend C. M., Jr, Thompson J. C. Neurotensin expression and release in human colon cancers. Ann Surg. 1992 Oct;216(4):423–431. doi: 10.1097/00000658-199210000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Evers B. M., Ishizuka J., Townsend C. M., Jr, Rajaraman S., Thompson J. C. Expression of neurotensin messenger RNA in a human carcinoid tumor. Ann Surg. 1991 Oct;214(4):448–455. doi: 10.1097/00000658-199110000-00009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Evers B. M., Izukura M., Chung D. H., Parekh D., Yoshinaga K., Greeley G. H., Jr, Uchida T., Townsend C. M., Jr, Thompson J. C. Neurotensin stimulates growth of colonic mucosa in young and aged rats. Gastroenterology. 1992 Jul;103(1):86–91. doi: 10.1016/0016-5085(92)91099-p. [DOI] [PubMed] [Google Scholar]
  20. Evers B. M., Izukura M., Townsend C. M., Jr, Uchida T., Thompson J. C. Neurotensin prevents intestinal mucosal hypoplasia in rats fed an elemental diet. Dig Dis Sci. 1992 Mar;37(3):426–431. doi: 10.1007/BF01307738. [DOI] [PubMed] [Google Scholar]
  21. Izukura M., Evers B. M., Parekh D., Yoshinaga K., Uchida T., Townsend C. M., Jr, Thompson J. C. Neurotensin augments intestinal regeneration after small bowel resection in rats. Ann Surg. 1992 May;215(5):520–527. doi: 10.1097/00000658-199205000-00015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kapuscinski M., Shulkes A., Read D., Hardy K. J. Expression of neurotensin in endocrine tumors. J Clin Endocrinol Metab. 1990 Jan;70(1):100–106. doi: 10.1210/jcem-70-1-100. [DOI] [PubMed] [Google Scholar]
  23. Kislauskis E., Bullock B., McNeil S., Dobner P. R. The rat gene encoding neurotensin and neuromedin N. Structure, tissue-specific expression, and evolution of exon sequences. J Biol Chem. 1988 Apr 5;263(10):4963–4968. [PubMed] [Google Scholar]
  24. Lee Y. C., Bacarese-Hamilton A. J., Wood S. M., Blumgart L. H., Bloom S. R. Chromatographic characterisation of neurotensin-like immunoreactivity in plasma and tissue extracts from hepatoma patients. Clin Chim Acta. 1985 Jun 30;149(1):29–36. doi: 10.1016/0009-8981(85)90270-0. [DOI] [PubMed] [Google Scholar]
  25. Mitchell P. J., Tjian R. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science. 1989 Jul 28;245(4916):371–378. doi: 10.1126/science.2667136. [DOI] [PubMed] [Google Scholar]
  26. Powell D. J., Friedman J. M., Oulette A. J., Krauter K. S., Darnell J. E., Jr Transcriptional and post-transcriptional control of specific messenger RNAs in adult and embryonic liver. J Mol Biol. 1984 Oct 15;179(1):21–35. doi: 10.1016/0022-2836(84)90304-8. [DOI] [PubMed] [Google Scholar]
  27. Read D., Shulkes A., Fernley R., Simpson R. Characterization of neurotensin(6-13) from an hepatic fibrolamellar carcinoma. Peptides. 1991 Jul-Aug;12(4):887–892. doi: 10.1016/0196-9781(91)90152-f. [DOI] [PubMed] [Google Scholar]
  28. Reinecke M. Neurotensin. Immunohistochemical localization in central and peripheral nervous system and in endocrine cells and its functional role as neurotransmitter and endocrine hormone. Prog Histochem Cytochem. 1985;16(1):1–172. [PubMed] [Google Scholar]
  29. Soreide O., Czerniak A., Bradpiece H., Bloom S., Blumgart L. Characteristics of fibrolamellar hepatocellular carcinoma. A study of nine cases and a review of the literature. Am J Surg. 1986 Apr;151(4):518–523. doi: 10.1016/0002-9610(86)90117-0. [DOI] [PubMed] [Google Scholar]
  30. Tanaka M., Sasaki H., Kino I., Sugimura T., Terada M. Genes preferentially expressed in embryo stomach are predominantly expressed in gastric cancer. Cancer Res. 1992 Jun 15;52(12):3372–3377. [PubMed] [Google Scholar]
  31. Thor K., Rosell S. Neurotensin increases colonic motility. Gastroenterology. 1986 Jan;90(1):27–31. doi: 10.1016/0016-5085(86)90070-3. [DOI] [PubMed] [Google Scholar]
  32. Wood J. G., Hoang H. D., Bussjaeger L. J., Solomon T. E. Neurotensin stimulates growth of small intestine in rats. Am J Physiol. 1988 Dec;255(6 Pt 1):G813–G817. doi: 10.1152/ajpgi.1988.255.6.G813. [DOI] [PubMed] [Google Scholar]

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