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. 1986 Mar;203(3):318–328. doi: 10.1097/00000658-198603000-00019

Platelet deposition on vascular grafts. The accuracy of in vivo quantitation and the significance of in vivo platelet reactivity.

B T Allen, C J Mathias, G A Sicard, M J Welch, R E Clark
PMCID: PMC1251099  PMID: 2937375

Abstract

An in vivo platelet imaging system utilizing indium-111-labeled platelets and technetium-99m-labeled red cells was used to serially study and compare platelet deposition on autologous external jugular vein grafts, autologous arterial grafts, polytetrafluoroethylene (Gore-tex) and two Dacron (Meadox and USCI) small diameter (4mm) vascular grafts implanted end-to-end in canine carotid and femoral arteries. This method of quantitating platelet deposition was validated by correlating deposition measured in vivo with deposition measured directly on explanted grafts (r = 0.94, p less than 0.01). Platelet accumulation on all grafts was greatest immediately after implantation and declined over time. None of the artery or vein grafts thrombosed, and they had the lowest level of platelet deposition at all times. Platelet deposition on Gore-tex grafts was significantly less than on USCI Dacron grafts from 24 hours to 1 month after implantation. There was no statistical difference in 1-month patency among the synthetic graft groups. Synthetic grafts that thrombosed during the first month accumulated significantly more platelets immediately after operation than did those grafts that remained patent. Patent Dacron grafts with low levels of platelet deposition had less thrombotic debris at explantation on the luminal surface than did those grafts with high levels of platelet deposition. Differences in initial platelet deposition appeared to be more a function of platelet reactivity within each dog rather than the material used in graft construction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen B. T., Long J. A., Clark R. E., Sicard G. A., Hopkins K. T., Welch M. J. Influence of endothelial cell seeding on platelet deposition and patency in small-diameter Dacron arterial grafts. J Vasc Surg. 1984 Jan;1(1):224–233. [PubMed] [Google Scholar]
  2. Allen B. T., Sparks R. E., Welch M. J., Mason N. S., Mathias C. J., Clark R. E. Reduction of platelet deposition on vascular grafts using an antiplatelet graft coating technique. J Surg Res. 1984 Jan;36(1):80–88. doi: 10.1016/0022-4804(84)90070-2. [DOI] [PubMed] [Google Scholar]
  3. BROWN R. B., KOTH D. R., URSCHEL H. C., Jr, ROTH E. J. Further studies of small vessel anastomosis and replacement in the dog. I. Plastic prostheses. II. An unidentified host variable affecting results. Surgery. 1960 Jun;47:987–997. [PubMed] [Google Scholar]
  4. Christenson J. T., Megerman J., Hanel K. C., L'Italien G. J., Strauss H. W., Abbott W. M. The effect of blood flow rates on platelet deposition in PTFE arterial bypass grafts. Trans Am Soc Artif Intern Organs. 1981;27:188–191. [PubMed] [Google Scholar]
  5. Clagett G. P., Robinowitz M., Maddox Y., Langloss J. M., Ramwell P. W. The antithrombotic nature of vascular prosthetic pseudointima. Surgery. 1982 Jan;91(1):87–94. [PubMed] [Google Scholar]
  6. Dean R. H., Yao J. S., Stanton P. E., Bergan J. J. Prognostic indicators in femoropopliteal reconstructions. Arch Surg. 1975 Nov;110(11):1287–1293. doi: 10.1001/archsurg.1975.01360170027003. [DOI] [PubMed] [Google Scholar]
  7. Fonkalsrud E. W., Sanchez M., Zerubavel R. Morphological evaluation of canine autogeneous vein grafts in the arterial circulation. Surgery. 1978 Aug;84(2):253–264. [PubMed] [Google Scholar]
  8. Fuster V., Dewanjee M. K., Kaye M. P., Josa M., Metke M. P., Chesebro J. H. Noninvasive radioisotopic technique for detection of platelet deposition in coronary artery bypass grafts in dogs and its reduction with platelet inhibitors. Circulation. 1979 Dec;60(7):1508–1512. doi: 10.1161/01.cir.60.7.1508. [DOI] [PubMed] [Google Scholar]
  9. Goldman M., Hall C., Dykes J., Hawker R. J., McCollum C. N. Does 111indium-platelet deposition predict patency in prosthetic arterial grafts? Br J Surg. 1983 Oct;70(10):635–638. doi: 10.1002/bjs.1800701019. [DOI] [PubMed] [Google Scholar]
  10. Hanel K. C., McCabe C., Abbott W. M., Fallon J., Megerman J. Current PTFE grafts: a biomechanical, scanning electron, and light microscopic evaluation. Ann Surg. 1982 Apr;195(4):456–463. doi: 10.1097/00000658-198204000-00012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Harajola P. T., Meurala H., Frick M. H. Prevention of early reocclusion by dipyridamole and ASA in arterial reconstructive surgery. J Cardiovasc Surg (Torino) 1981 Mar-Apr;22(2):141–144. [PubMed] [Google Scholar]
  12. Heaton W. A., Davis H. H., Welch M. J., Mathias C. J., Joist J. H., Sherman L. A., Siegel B. A. Indium-III: a new radionuclide label for studying human platelet kinetics. Br J Haematol. 1979 Aug;42(4):613–622. doi: 10.1111/j.1365-2141.1979.tb01174.x. [DOI] [PubMed] [Google Scholar]
  13. Kenny D. A., Berger K., Walker M. W., Robel S. B., Boguslavsky L., Ray L. I., Lischko M. M., Sauvage L. R. Experimental comparison of the thrombogenicity of fibrin and PTFE flow surfaces. Ann Surg. 1980 Mar;191(3):355–361. doi: 10.1097/00000658-198003000-00016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oblath R. W., Buckley F. O., Jr, Green R. M., Schwartz S. I., DeWeese J. A. Prevention of platelet aggregation and adherence to prosthetic vascular grafts by aspirin and dipyridamole. Surgery. 1978 Jul;84(1):37–44. [PubMed] [Google Scholar]
  15. Powers W. J., Hopkins K. T., Welch M. J. Validation of the dual radiotracer method for quantitative In-111 platelet scintigraphy. Thromb Res. 1984 Apr 15;34(2):135–145. doi: 10.1016/0049-3848(84)90070-7. [DOI] [PubMed] [Google Scholar]
  16. Roedersheimer L. R., Cohn M. S., Grauer S. E., Green R. M., De Weese J. A. Goretex and autogenous vein in the canine model. J Cardiovasc Surg (Torino) 1980 Sep-Oct;21(5):581–589. [PubMed] [Google Scholar]
  17. Ross R., Glomset J., Kariya B., Harker L. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1207–1210. doi: 10.1073/pnas.71.4.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwartz K. D., Kruger M., Weinstein M. B. Improvement in labeling erythrocytes with 99mTc-pertechnetate. J Nucl Med. 1971 Jun;12(6):323–325. [PubMed] [Google Scholar]
  19. Sharp W. V. The carotid artery--a test site for small vessel prosthetics. J Surg Res. 1970 Jan;10(1):41–46. doi: 10.1016/0022-4804(70)90008-9. [DOI] [PubMed] [Google Scholar]
  20. Sicard G. A., Allen B. T., Long J. A., Welch M. J., Griffin A., Clark R. E., Anderson C. B. Prostaglandin production and platelet reactivity of small-diameter grafts. J Vasc Surg. 1984 Nov;1(6):774–781. [PubMed] [Google Scholar]
  21. Stanley J. C., Burkel W. E., Ford J. W., Vinter D. W., Kahn R. H., Whitehouse W. M., Jr, Graham L. M. Enhanced patency of small-diameter, externally supported Dacron iliofemoral grafts seeded with endothelial cells. Surgery. 1982 Dec;92(6):994–1005. [PubMed] [Google Scholar]
  22. Yates S. G., Barros D'Sa A. A., Berger K., Fernandez L. G., Wood S. J., Rittenhouse E. A., Davis C. C., Mansfield P. B., Sauvage L. R. The preclotting of porous arterial prostheses. Ann Surg. 1978 Nov;188(5):611–622. doi: 10.1097/00000658-197811000-00005. [DOI] [PMC free article] [PubMed] [Google Scholar]

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