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. 1986 Jul;204(1):83–88. doi: 10.1097/00000658-198607000-00012

Acute intestinal ischemia studies by phosphorus nuclear magnetic resonance spectroscopy.

H Blum, J J Summers, M D Schnall, C Barlow, J S Leigh Jr, B Chance, G P Buzby
PMCID: PMC1251227  PMID: 3729587

Abstract

31P nuclear magnetic resonance (NMR) spectroscopy has been used to follow the metabolism of acutely ischemic rat small intestine and its recovery after reversal of ischemia. Loops of small intestine were subjected to occlusive external pressure for up to 60 minutes, followed by a recovery period. The depletion of PCr and ATP is rapid and complete within 20 minutes. Recovery from ischemia is also rapid but with recovery ATP levels lower than initial values after prolonged ischemic periods. Intestinal shock was avoided. Clinical recovery correlated with shorter ischemic periods. 31P NMR spectroscopy thus appears to be a suitable technique for studying the effects of pharmacological agents and other treatments for amelioration of ischemic effects on the bowel.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson R., Pärsson H., Isaksson B., Norgren L. Acute intestinal ischemia. A 14-year retrospective investigation. Acta Chir Scand. 1984;150(3):217–221. [PubMed] [Google Scholar]
  2. Bennion R. S., Wilson S. E., Serota A. I., Williams R. A. The role of gastrointestinal microflora in the pathogenesis of complications of mesenteric ischemia. Rev Infect Dis. 1984 Mar-Apr;6 (Suppl 1):S132–S138. doi: 10.1093/clinids/6.supplement_1.s132. [DOI] [PubMed] [Google Scholar]
  3. Boley S. J., Sprayregan S., Siegelman S. S., Veith F. J. Initial results from an agressive roentgenological and surgical approach to acute mesenteric ischemia. Surgery. 1977 Dec;82(6):848–855. [PubMed] [Google Scholar]
  4. Brooks W. M., Willis R. J. 31P nuclear magnetic resonance study of the recovery characteristics of high energy phosphate compounds and intracellular pH after global ischaemia in the perfused guinea-pig heart. J Mol Cell Cardiol. 1983 Aug;15(8):495–502. doi: 10.1016/0022-2828(83)90325-5. [DOI] [PubMed] [Google Scholar]
  5. Budinger T. F., Lauterbur P. C. Nuclear magnetic resonance technology for medical studies. Science. 1984 Oct 19;226(4672):288–298. doi: 10.1126/science.6385252. [DOI] [PubMed] [Google Scholar]
  6. CAMERON G. R., KHANNA S. D. Regeneration of the intestinal villi after extensive mucosal infarction. J Pathol Bacteriol. 1959 Apr;77(2):505–510. doi: 10.1002/path.1700770222. [DOI] [PubMed] [Google Scholar]
  7. Cronenwett J. L., Ayad M., Kazmers A. Effect of intravenous glucagon on the survival of rats after acute occlusive mesenteric ischemia. J Surg Res. 1985 May;38(5):446–452. doi: 10.1016/0022-4804(85)90060-5. [DOI] [PubMed] [Google Scholar]
  8. Demetriou A. A., Kagoma P. K., Kaiser S., Seifter E., Niu X. T., Levenson S. M. Effect of dimethyl sulfoxide and glycerol on acute bowel ischemia in the rat. Am J Surg. 1985 Jan;149(1):91–94. doi: 10.1016/s0002-9610(85)80015-5. [DOI] [PubMed] [Google Scholar]
  9. Fondacaro J. D., Walus K. M., Schwaiger M., Jacobson E. D. Vasodilation of the normal and ischemic canine mesenteric circulation. Gastroenterology. 1981 Jun;80(6):1542–1549. [PubMed] [Google Scholar]
  10. Gadian D. G., Radda G. K. NMR studies of tissue metabolism. Annu Rev Biochem. 1981;50:69–83. doi: 10.1146/annurev.bi.50.070181.000441. [DOI] [PubMed] [Google Scholar]
  11. Gordon R. E., Hanley P. E., Shaw D., Gadian D. G., Radda G. K., Styles P., Bore P. J., Chan L. Localization of metabolites in animals using 31P topical magnetic resonance. Nature. 1980 Oct 23;287(5784):736–738. doi: 10.1038/287736a0. [DOI] [PubMed] [Google Scholar]
  12. Gustafsson G. Effects of dopamine in segmental intestinal ischaemia studied with dopamine receptor blockers. Acta Chir Scand. 1984;150(2):145–151. [PubMed] [Google Scholar]
  13. Gustafsson G., Norlén K., Rentzhog L., Wikström S. Delayed dopamine treatment of segmental small intestinal ischaemia in the rat. Acta Chir Scand. 1984;150(2):153–158. [PubMed] [Google Scholar]
  14. Gustafsson G., Norlén K., Rentzhog L., Wikström S. Haemodynamic effects of phenoxybenzamine and dopamine in segmental ischaemia of the rat small intestine. Acta Chir Scand. 1984;150(2):159–163. [PubMed] [Google Scholar]
  15. Haglind E., Haglund U., Lundgren O., Romanus M., Scherstén T. Graded intestinal vascular obstruction: I. Description of an experimental shock model in the rat. Circ Shock. 1980;7(1):83–91. [PubMed] [Google Scholar]
  16. Haglund U., Myrvold H., Lundgren O. Cardiac and pulmonary function in regional intestinal shock. Arch Surg. 1978 Aug;113(8):963–969. doi: 10.1001/archsurg.1978.01370200057011. [DOI] [PubMed] [Google Scholar]
  17. Haselgrove J. C., Subramanian V. H., Leigh J. S., Jr, Gyulai L., Chance B. In vivo one-dimensional imaging of phosphorus metabolites by phosphorus-31 nuclear magnetic resonance. Science. 1983 Jun 10;220(4602):1170–1173. doi: 10.1126/science.6857240. [DOI] [PubMed] [Google Scholar]
  18. Hollis D. P., Nunnally R. L., Jacobus W. E., Taylor G. J., 4th Detection of regional ischemia in perfused beating hearts by phosphorus nuclear magnetic resonance. Biochem Biophys Res Commun. 1977 Apr 25;75(4):1086–1091. doi: 10.1016/0006-291x(77)91493-0. [DOI] [PubMed] [Google Scholar]
  19. Ingwall J. S. Phosphorus nuclear magnetic resonance spectroscopy of cardiac and skeletal muscles. Am J Physiol. 1982 May;242(5):H729–H744. doi: 10.1152/ajpheart.1982.242.5.H729. [DOI] [PubMed] [Google Scholar]
  20. Komaki R., Wilson J. F., Cox J. D., Kline R. W. Carcinoma of the pancreas: results of irradiation for unresectable lesions. Int J Radiat Oncol Biol Phys. 1980 Feb;6(2):209–212. doi: 10.1016/0360-3016(80)90039-5. [DOI] [PubMed] [Google Scholar]
  21. Krausz M. M., Manny J. Acute superior mesenteric arterial occlusion: a plea for early diagnosis. Surgery. 1978 Apr;83(4):482–485. [PubMed] [Google Scholar]
  22. Maris J. M., Evans A. E., McLaughlin A. C., D'Angio G. J., Bolinger L., Manos H., Chance B. 31P nuclear magnetic resonance spectroscopic investigation of human neuroblastoma in situ. N Engl J Med. 1985 Jun 6;312(23):1500–1505. doi: 10.1056/NEJM198506063122307. [DOI] [PubMed] [Google Scholar]
  23. Maudsley A. A., Hilal S. K., Simon H. E., Wittekoek S. In vivo MR spectroscopic imaging with P-31. Work in progress. Radiology. 1984 Dec;153(3):745–750. doi: 10.1148/radiology.153.3.6494470. [DOI] [PubMed] [Google Scholar]
  24. Ohkawa M., Chaudry I. H., Clemens M. G., Baue A. E. ATP-MgCl2 produces sustained improvement in hepatic mitochondrial function and blood flow after hepatic ischemia. J Surg Res. 1984 Sep;37(3):226–234. doi: 10.1016/0022-4804(84)90183-5. [DOI] [PubMed] [Google Scholar]
  25. Ottinger L. W., Austen W. G. A study of 136 patients with mesenteric infarction. Surg Gynecol Obstet. 1967 Feb;124(2):251–261. [PubMed] [Google Scholar]
  26. Pitha J. The fine structure of regenerating epithelium in the small intestine. Virchows Arch B Cell Pathol. 1971;7(4):314–341. [PubMed] [Google Scholar]
  27. Quistorff B., Engkagul A., Chance B. 31-P NMR in the study of liver metabolism in vivo. Pharmacol Biochem Behav. 1983;18 (Suppl 1):241–244. doi: 10.1016/0091-3057(83)90179-x. [DOI] [PubMed] [Google Scholar]
  28. Ricci J. L., Sloviter H. A., Ziegler M. M. Intestinal ischemia: reduction of mortality utilizing intraluminal perfluorochemical. Am J Surg. 1985 Jan;149(1):84–90. doi: 10.1016/s0002-9610(85)80014-3. [DOI] [PubMed] [Google Scholar]
  29. Roberts J. K., Jardetzky O. Monitoring of cellular metabolism by NMR. Biochim Biophys Acta. 1981 Nov 9;639(1):53–76. doi: 10.1016/0304-4173(81)90005-7. [DOI] [PubMed] [Google Scholar]
  30. Robinson J. W., Mirkovitch V., Winistörfer B., Saegesser F. Response of the intestinal mucosa to ischaemia. Gut. 1981 Jun;22(6):512–527. doi: 10.1136/gut.22.6.512. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Seemann W. R., Mathias K., Roeren T., Urbanyi B., Kopp K. H. Adenosine and diltiazem. A new therapeutic concept in the treatment of intestinal ischemia. Invest Radiol. 1985 Mar-Apr;20(2):166–170. [PubMed] [Google Scholar]
  32. Sehr P. A., Bore P. J., Papatheofanis J., Radda G. K. Non-destructive measurement of metabolites and tissue pH in the kidney by 31P nuclear magnetic resonance. Br J Exp Pathol. 1979 Dec;60(6):632–641. [PMC free article] [PubMed] [Google Scholar]
  33. Shapiro D. M., Cronenwett J. L., Lindenauer S. M., Luce J. L., Stanley J. C. Effects of glucagon and prostacyclin in acute occlusive and postocclusive canine mesenteric ischemia. J Surg Res. 1984 Jun;36(6):535–546. doi: 10.1016/0022-4804(84)90139-2. [DOI] [PubMed] [Google Scholar]
  34. Siegel N. J., Avison M. J., Reilly H. F., Alger J. R., Shulman R. G. Enhanced recovery of renal ATP with postischemic infusion of ATP-MgCl2 determined by 31P-NMR. Am J Physiol. 1983 Oct;245(4):F530–F534. doi: 10.1152/ajprenal.1983.245.4.F530. [DOI] [PubMed] [Google Scholar]
  35. Singh R. P., Lee S. T. Acute mesenteric vascular occlusion: a review of 40 cases. Int Surg. 1980 May-Jun;65(3):231–234. [PubMed] [Google Scholar]
  36. Styles P., Scott C. A., Radda G. K. A method for localizing high-resolution NMR spectra from human subjects. Magn Reson Med. 1985 Aug;2(4):402–409. doi: 10.1002/mrm.1910020408. [DOI] [PubMed] [Google Scholar]
  37. Sumpio B. E., Chaudry I. H., Baue A. E. Adenosine triphosphate--magnesium chloride ameliorates reperfusion injury following ischemia as determined by phosphorus nuclear magnetic resonance. Arch Surg. 1985 Feb;120(2):233–240. doi: 10.1001/archsurg.1985.01390260091013. [DOI] [PubMed] [Google Scholar]
  38. Sumpio B. E., Chaudry I. H., Clemens M. G., Baue A. E. Accelerated functional recovery of isolated rat kidney with ATP-MgCl2 after warm ischemia. Am J Physiol. 1984 Dec;247(6 Pt 2):R1047–R1053. doi: 10.1152/ajpregu.1984.247.6.R1047. [DOI] [PubMed] [Google Scholar]
  39. Whitman G. J., Harken A. H. Applications of nuclear magnetic resonance to surgical disease: a collective review. J Surg Res. 1985 Feb;38(2):187–199. doi: 10.1016/0022-4804(85)90026-5. [DOI] [PubMed] [Google Scholar]
  40. Zanotti A. M., Gaffin S. L. Prophylaxis of superior mesenteric artery occlusion shock in rabbits by antilipopolysaccharide (anti-LPS) antibodies. J Surg Res. 1985 Feb;38(2):113–115. doi: 10.1016/0022-4804(85)90015-0. [DOI] [PubMed] [Google Scholar]

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