Skip to main content
PMC home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1987 Jan;51(1):32–38.

Vaccination of calves with a diaminopimelic acid mutant of Salmonella typhimurium.

R C Clarke, C L Gyles
PMCID: PMC1255270  PMID: 3032389

Abstract

The purpose of this study was to evaluate the safety and efficacy of a diaminopimelic acid mutant of Salmonella typhimurium as a vaccine for calves. Transposon techniques were used to create a stable nonreverting diaminopimelic acid mutant of a virulent S. typhimurium strain. Calves were vaccinated at weekly intervals with the diaminopimelic acid mutant given as an oral dose of 10(10) organisms, followed by two subcutaneous doses of 10(9) organisms. The calves tolerated vaccination well and the vaccine strain was eliminated from the feces within four days. Of five vaccinated calves, three survived challenge with 5 X 10(9) organisms of the parent strain whereas all five unvaccinated calves that were challenged died. The surviving calves eliminated the challenge organism from the feces within three weeks.

Full text

PDF
32

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angerman C. R., Eisenstein T. K. Comparative efficacy and toxicity of a ribosomal vaccine, acetone-killed cells, lipopolysaccharide, and a live cell vaccine prepared from Salmonella typhhimurium. Infect Immun. 1978 Feb;19(2):575–582. doi: 10.1128/iai.19.2.575-582.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baljer G., Hoerstke M., Dirksen G., Seitz A., Sailer J., Mayr A. Vergleichende Untersuchungen über die Wirksamkeit einer oralen Immuniseirung mit hitzeinaktivierten und vermehrungsfähigen, avirulenten (Gal E-) S. typhimurium-Keimen gegen die Salmonellose des Kalbes. Zentralbl Veterinarmed B. 1981;28(9-10):759–766. [PubMed] [Google Scholar]
  3. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown S. L., Klein G. C., McKinney F. T., Jones W. L. Safranin O-stained antigen microagglutination test for detection of brucella antibodies. J Clin Microbiol. 1981 Feb;13(2):398–400. doi: 10.1128/jcm.13.2.398-400.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clarke R. C., Gyles C. L. Galactose epimeraseless mutants of Salmonella typhimurium as live vaccines for calves. Can J Vet Res. 1986 Apr;50(2):165–173. [PMC free article] [PubMed] [Google Scholar]
  6. Collins F. M., Campbell S. G. Immunity to intracellular bacteria. Vet Immunol Immunopathol. 1982 Jan;3(1-2):5–66. doi: 10.1016/0165-2427(82)90031-9. [DOI] [PubMed] [Google Scholar]
  7. Curtiss R., 3rd Biological containment and cloning vector transmissibility. J Infect Dis. 1978 May;137(5):668–675. doi: 10.1093/infdis/137.5.668. [DOI] [PubMed] [Google Scholar]
  8. Killar L. M., Eisenstein T. K. Immunity to Salmonella typhimurium infection in C3H/HeJ and C3H/HeNCrlBR mice: studies with an aromatic-dependent live S. typhimurium strain as a vaccine. Infect Immun. 1985 Mar;47(3):605–612. doi: 10.1128/iai.47.3.605-612.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Robertsson J. A., Lindberg A. A., Hoiseth S., Stocker B. A. Salmonella typhimurium infection in calves: protection and survival of virulent challenge bacteria after immunization with live or inactivated vaccines. Infect Immun. 1983 Aug;41(2):742–750. doi: 10.1128/iai.41.2.742-750.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SMITH H. W. THE IMMUNIZATION OF MICE, CALVES AND PIGS AGAINST SALMONELLA DUBLIN AND SALMONELLA CHOLERAE-SUIS INFECTIONS. J Hyg (Lond) 1965 Mar;63:117–135. doi: 10.1017/s0022172400045022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith B. P., Habasha F. G., Reina-Guierra M., Hardy A. J. Immunization of calves against salmonellosis. Am J Vet Res. 1980 Dec;41(12):1947–1951. [PubMed] [Google Scholar]
  12. Smith B. P., Reina-Guerra M., Hoiseth S. K., Stocker B. A., Habasha F., Johnson E., Merritt F. Aromatic-dependent Salmonella typhimurium as modified live vaccines for calves. Am J Vet Res. 1984 Jan;45(1):59–66. [PubMed] [Google Scholar]
  13. Stocker B. A., Hoiseth S. K., Smith B. P. Aromatic-dependent "Salmonella sp." as live vaccine in mice and calves. Dev Biol Stand. 1983;53:47–54. [PubMed] [Google Scholar]
  14. Ushiba D. Two types of immunity in experimental typhoid; "cellular immunity" and "humoral immunity". Keio J Med. 1965 Jun;14(2):45–61. doi: 10.2302/kjm.14.45. [DOI] [PubMed] [Google Scholar]
  15. Wahdan M. H., Serie C., Germanier R., Lackany A., Cerisier Y., Guerin N., Sallam S., Geoffroy P., el Tantawi A. S., Guesry P. A controlled field trial of liver oral typhoid vaccine Ty21a. Bull World Health Organ. 1980;58(3):469–474. [PMC free article] [PubMed] [Google Scholar]
  16. Wray C., Sojka W. J., Pritchard D. G., Morris J. A. Immunization of animals with gal E mutants of "Salmonella typhimurium". Dev Biol Stand. 1983;53:41–46. [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES