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Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1987 Jan;51(1):126–134.

Changes in N-acetyl-B-D-glucosaminidase and B-glucuronidase activities in milk during bovine mastitis.

H Nagahata, S Saito, H Noda
PMCID: PMC1255286  PMID: 3567747

Abstract

To determine the N-acetyl-B-D-glucosaminidase (NAGase) and B-glucuronidase (B-Gase) activities in mastitic milk, basic enzyme assay conditions, distribution of NAGase and B-Gase, comparison of their activities with California Mastitis Test scores, and the effects of the milking process on their enzyme activities were examined. The mean NAGase and B-Gase activities in milk macrophages were about threefold higher than those of milk and blood polymorphonuclear cells. Very little NAGase activity appeared to be associated with blood mononuclear cells, whereas a relatively higher B-Gase activity was observed. California Mastitis Test scores of each group (1 to 5) appeared to be well correlated (r = 0.86 for NAGase and 0.92 for B-Gase) with the levels of NAGase and B-Gase activity. The milking process was least effective in the normal milk, but some variations of enzyme activities during milking in mastitic milk were found. Changes in NAGase and B-Gase activities in quarter milk were well monitored during the course of clinical mastitis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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