Skip to main content
PMC home page
Journal of Anatomy logoLink to Journal of Anatomy
. 1990 Aug;171:223–231.

A survey of endocrine cells in the pancreas of the echidna (Tachyglossus aculeatus) with special reference to pancreatic motilin cells.

J Yamada 1, W J Krause 1, N Edwin 1, T Mochizuki 1, N Yanaihara 1
PMCID: PMC1257143  PMID: 1981999

Abstract

Pancreatic endocrine cells were examined in a primitive egg-laying mammal, the echidna, using immunohistochemistry. Immunoreactive endocrine cells were observed using antisera to insulin, glucagon, somatostatin, avian pancreatic polypeptide and bovine pancreatic polypeptide. In addition, motilin-immunoreactive cells were identified in both the endocrine and exocrine pancreas of pouch-young and adult echidnas using three types of motilin antisera. Since the motilin-immunoreactive cells did not cross-react with any other pancreatic hormones tested, they are identified as an independent endocrine cell type.

Full text

PDF
228

Images in this article

225Fig. 1
on p.225
225Fig. 2
on p.225
226Fig. 3
on p.226
226Fig. 4
on p.226
227Fig. 5
on p.227
227Fig. 6
on p.227
228Fig. 7
on p.228

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown J. C., Mutt V., Dryburgh J. R. The further purification of motilin, a gastric motor activity stimulating polypeptide from the mucosa of the small intestine of hogs. Can J Physiol Pharmacol. 1971 May;49(5):399–405. doi: 10.1139/y71-047. [DOI] [PubMed] [Google Scholar]
  2. Buchan A. M., Lance V., Polak J. M. The endocrine pancreas of Alligator mississippiensis. An immunocytochemical investigation. Cell Tissue Res. 1982;224(1):117–128. doi: 10.1007/BF00217271. [DOI] [PubMed] [Google Scholar]
  3. Christofides N. D., Modlin I. M., Fitzpatrick M. L., Bloom S. R. Effect of motilin on the rate of gastric emptying and gut hormone release during breakfast. Gastroenterology. 1979 May;76(5 Pt 1):903–907. [PubMed] [Google Scholar]
  4. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  5. Jacobowitz D. M., O'Donohue T. L., Chey W. Y., Chang T. M. Mapping of motilin-immunoreactive neurons of the rat brain. Peptides. 1981 Winter;2(4):479–487. doi: 10.1016/s0196-9781(81)80107-6. [DOI] [PubMed] [Google Scholar]
  6. Krause W. J., Cutts J. H., 3rd, Cutts J. H., Yamada J. Immunohistochemical study of the developing endocrine pancreas of the opossum (Didelphis virginiana). Acta Anat (Basel) 1989;135(1):84–96. doi: 10.1159/000146727. [DOI] [PubMed] [Google Scholar]
  7. Krause W. J. Light and electron microscopic studies on the gastrointestinal tract of the suckling echidna (Tachyglossus aculeatus). Anat Rec. 1972 Apr;172(4):603–621. doi: 10.1002/ar.1091720402. [DOI] [PubMed] [Google Scholar]
  8. Krause W. J., Yamada J. A brief report on enteroendocrine cells observed in the gastrointestinal tract of the suckling echidna (Tachyglossus aculeatus). Z Mikrosk Anat Forsch. 1986;100(3):464–468. [PubMed] [Google Scholar]
  9. Larsson L. I., Sundler F., Håkanson R. Pancreatic polypeptide - a postulated new hormone: identification of its cellular storage site by light and electron microscopic immunocytochemistry. Diabetologia. 1976 Jul;12(3):211–226. doi: 10.1007/BF00422088. [DOI] [PubMed] [Google Scholar]
  10. Matsuzaki H., Yamada J., Krause W. J., Kitamura N., Yamashita T. An ultrastructural and immunocytochemical study on endocrine cells in the proximal duodenum of the echidna (Tachyglossus aculeatus). Z Mikrosk Anat Forsch. 1985;99(5):793–803. [PubMed] [Google Scholar]
  11. Nilaver G., Beinfeld M. C., Bond C. T., Daikh D., Godfrey B., Adelman J. P. Heterogeneity of motilin immunoreactivity in mammalian tissue. Synapse. 1988;2(3):266–275. doi: 10.1002/syn.890020315. [DOI] [PubMed] [Google Scholar]
  12. Orci L., Baetens D., Ravazzola M., Stefan Y., Malaisse-Lagae F. Pancreatic polypeptide and glucagon : non-random distribution in pancreatic islets. Life Sci. 1976 Dec 15;19(12):1811–1815. doi: 10.1016/0024-3205(76)90112-0. [DOI] [PubMed] [Google Scholar]
  13. Pearse A. G., Polak J. M., Bloom S. R., Adams C., Dryburgh J. R., Brown J. C. Enterochromaffin cells of the mammalian small intestine as the source of motilin. Virchows Arch B Cell Pathol. 1974;16(2):111–120. doi: 10.1007/BF02894069. [DOI] [PubMed] [Google Scholar]
  14. Reddy S., Bibby N. J., Fisher S. L., Elliott R. B. Immunolocalization of insulin, glucagon, pancreatic polypeptide, and somatostatin in the pancreatic islets of the possum, Trichosurus vulpecula. Gen Comp Endocrinol. 1986 Oct;64(1):157–162. doi: 10.1016/0016-6480(86)90042-0. [DOI] [PubMed] [Google Scholar]
  15. Rhoten W. B. Quantitative immunocytochemical analysis of the endocrine pancreas of the Nile crocodile. Am J Anat. 1987 Feb;178(2):103–115. doi: 10.1002/aja.1001780202. [DOI] [PubMed] [Google Scholar]
  16. Smith P. H., Davis B. J., Seino Y., Yanaihara N. Localization of motilin-containing cells in the intestinal tract of mammals: a further comparison using region-specific motilin antisera. Gen Comp Endocrinol. 1981 Jul;44(3):288–291. doi: 10.1016/0016-6480(81)90003-4. [DOI] [PubMed] [Google Scholar]
  17. Yamada J., Matsuzaki H., Kitamura N., Yamashita T., Krause W. J. An immunohistochemical survey of endocrine cells and nerves in the proximal duodenum of the echidna, Tachyglossus aculeatus. Z Mikrosk Anat Forsch. 1985;99(2):209–218. [PubMed] [Google Scholar]

Articles from Journal of Anatomy are provided here courtesy of Anatomical Society of Great Britain and Ireland

RESOURCES