Abstract
The estimation of the number of channels in a patch was assumed to be equivalent to the estimation of the binomial parameter n. Seven estimators were evaluated, using data sets simulated for a range of parameters appropriate for single channel recording experiments. No single estimator was best for all parameters; a combination of estimators is a possible option to avoid the biases of individual estimators. All estimators were highly accurate in estimating n in the case that n = 1. For n ≤ 4 the simplest estimator, the maximum number of simultaneously open channels, was the best, For larger values of n the best estimators were Bayesian.
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Selected References
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- Blatz A. L., Magleby K. L. Quantitative description of three modes of activity of fast chloride channels from rat skeletal muscle. J Physiol. 1986 Sep;378:141–174. doi: 10.1113/jphysiol.1986.sp016212. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colquhoun D., Hawkes A. G. Stochastic properties of ion channel openings and bursts in a membrane patch that contains two channels: evidence concerning the number of channels present when a record containing only single openings is observed. Proc R Soc Lond B Biol Sci. 1990 Jun 22;240(1299):453–477. doi: 10.1098/rspb.1990.0048. [DOI] [PubMed] [Google Scholar]
- Glasbey C. A., Martin R. J. The distribution of numbers of open channels in multi-channel patches. J Neurosci Methods. 1988 Jul;24(3):283–287. doi: 10.1016/0165-0270(88)90174-4. [DOI] [PubMed] [Google Scholar]
- Hess P., Lansman J. B., Tsien R. W. Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists. Nature. 1984 Oct 11;311(5986):538–544. doi: 10.1038/311538a0. [DOI] [PubMed] [Google Scholar]
- Horn R., Vandenberg C. A., Lange K. Statistical analysis of single sodium channels. Effects of N-bromoacetamide. Biophys J. 1984 Jan;45(1):323–335. doi: 10.1016/S0006-3495(84)84158-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jackson M. B. Stochastic behavior of a many-channel membrane system. Biophys J. 1985 Feb;47(2 Pt 1):129–137. doi: 10.1016/s0006-3495(85)83886-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liebovitch L. S., Fischbarg J. Determining the kinetics of membrane pores from patch clamp data without measuring the open and closed times. Biochim Biophys Acta. 1985 Feb 28;813(1):132–136. doi: 10.1016/0005-2736(85)90353-0. [DOI] [PubMed] [Google Scholar]
- Patlak J., Horn R. Effect of N-bromoacetamide on single sodium channel currents in excised membrane patches. J Gen Physiol. 1982 Mar;79(3):333–351. doi: 10.1085/jgp.79.3.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rösler K. M., Casanova G. A., Hess C. W., Meier C., Ludin H. P. Blasenstörungen bei jungen Erwachsenen mit Myelomeningocele: Ergebnisse urodynamisch-elektromyographischer Untersuchungen. Schweiz Med Wochenschr. 1990 Jul 28;120(30):1091–1097. [PubMed] [Google Scholar]
- Yeramian E., Trautmann A., Claverie P. Acetylcholine receptors are not functionally independent. Biophys J. 1986 Aug;50(2):253–263. doi: 10.1016/S0006-3495(86)83459-2. [DOI] [PMC free article] [PubMed] [Google Scholar]