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. 1991 Aug;60(2):440–446. doi: 10.1016/S0006-3495(91)82070-7

Correlation between absorption maxima and thermal isomerization rates in bacteriorhodopsin

Steven J Milder 1
PMCID: PMC1260081  PMID: 19431800

Abstract

The reported rates of thermal 13-cis to all-trans isomerization of the protonated Schiff base of retinal (PSBR) in solution and in bacteriorhodopsin (BR) are shown to be correlated with the red shift in the absorption maximum of the chromophore, though the linear fit is different for BR and for a model PSBR in solution. Because the red shift in the absorption has been previously shown to be correlated with π-electron delocalization in the chromophore, this suggests that the thermal isomerization rate is largely regulated by the amount of double bond character in the chromophore. Because the linear fit of isomerization rates with absorption maxima is different for BR and the model PSBR, specific interactions of the protein with the chromophore must also be a factor in determining thermal isomerization rates in BR. A model of the later steps in the photocycle of BR is presented in which the 13-cis to all-trans thermal isomerization occurs during the O intermediate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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