Abstract
In order to study the effects of paralysis on the development of bone, chick embryos were paralysed at 6 days of incubation and the pattern of histogenesis of the tibia was compared with that of control embryos by histology, scanning electron microscopy and morphometry. Up to 11 days of incubation the histological features of chondrification, initial perichondrial ossification and invasion by the vascular bud showed no differences. After this time the paralysed embryos exhibited a reduction in the formation of bone tissue and a reduced development of the fibular crest. The spread of cartilage resorption was also markedly reduced. In addition, scanning electron microscopy suggested a reduction in resorption of the innermost layers of bone. Blood vessels in the marrow cavity appeared smaller and those within developing osteones appeared larger than in the controls. In the paralysed embryos, morphometry confirmed a significant reduction in total volume of the tibia together with changes in its volumetric composition resulting from reduction in bone formation, cartilage formation and cartilage resorption.
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- Archer C. W., Ratcliffe N. A. The effects of pulsed magnetic fields on chick embryo cartilaginous skeletal rudiments in vitro. J Exp Zool. 1983 Feb;225(2):243–256. doi: 10.1002/jez.1402250208. [DOI] [PubMed] [Google Scholar]
- Bradley S. J. An analysis of self-differentiation of chick limb buds in chorio-allantoic grafts. J Anat. 1970 Nov;107(Pt 3):479–490. [PMC free article] [PubMed] [Google Scholar]
- Currey J. D. The adaptation of bones to stress. J Theor Biol. 1968 Jul;20(1):91–106. doi: 10.1016/0022-5193(68)90094-5. [DOI] [PubMed] [Google Scholar]
- Dillaman R. M., Wilbur K. M., Crenshaw M. A. Growth of embryonic chick tibiae in ovo and in vitro: a scanning electron microscope study. Calcif Tissue Int. 1979 Mar 13;27(1):33–40. doi: 10.1007/BF02441158. [DOI] [PubMed] [Google Scholar]
- Gaytan F., Ranz F. B., Aceitero J. Morphometric study of cartilage dynamics in the chick embryo tibia. I. Methodology and tissue compartments in normal embryos. J Anat. 1987 Oct;154:63–71. [PMC free article] [PubMed] [Google Scholar]
- Goodship A. E., Lanyon L. E., McFie H. Functional adaptation of bone to increased stress. An experimental study. J Bone Joint Surg Am. 1979 Jun;61(4):539–546. [PubMed] [Google Scholar]
- Hall B. K. A simple, single-injection method for inducing long-term paralysis in embryonic chicks, and preliminary observations on growth of the tibia. Anat Rec. 1975 Apr;181(4):767–777. doi: 10.1002/ar.1091810408. [DOI] [PubMed] [Google Scholar]
- Hall B. K. Cellular differentiation in skeletal tissues. Biol Rev Camb Philos Soc. 1970 Nov;45(4):455–484. doi: 10.1111/j.1469-185x.1970.tb01174.x. [DOI] [PubMed] [Google Scholar]
- Hosseini A., Hogg D. A. The effects of paralysis on skeletal development in the chick embryo. I. General effects. J Anat. 1991 Aug;177:159–168. [PMC free article] [PubMed] [Google Scholar]
- Lanyon L. E., Hampson W. G., Goodship A. E., Shah J. S. Bone deformation recorded in vivo from strain gauges attached to the human tibial shaft. Acta Orthop Scand. 1975 May;46(2):256–268. doi: 10.3109/17453677508989216. [DOI] [PubMed] [Google Scholar]
- Lutfi A. M. The fate of chondrocytes during cartilage erosion in the growing tibia in the domestic fowl (Gallus domesticus). Acta Anat (Basel) 1971;79(1):27–35. doi: 10.1159/000143620. [DOI] [PubMed] [Google Scholar]
- Lutfi A. M. The role of cartilage in long bone growth: a reappraisal. J Anat. 1974 Apr;117(Pt 2):413–417. [PMC free article] [PubMed] [Google Scholar]
- Scott-Savage P., Hall B. K. The timing of the onset of osteogenesis in the tibia of the embryonic chick. J Morphol. 1979 Dec;162(3):453–463. doi: 10.1002/jmor.1051620310. [DOI] [PubMed] [Google Scholar]
- Uchida A., Yamashita K., Hashimoto K., Shimomura Y. The effect of mechanical stress on cultured growth cartilage cells. Connect Tissue Res. 1988;17(4):305–311. doi: 10.3109/03008208809017480. [DOI] [PubMed] [Google Scholar]
- von der Mark H., von der Mark K., Gay S. Study of differential collagen synthesis during development of the chick embryo by immunofluorescence. I. Preparation of collagen type I and type II specific antibodies and their application to early stages of the chick embryo. Dev Biol. 1976 Feb;48(2):237–249. doi: 10.1016/0012-1606(76)90088-9. [DOI] [PubMed] [Google Scholar]