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. 1987 Oct;154:73–79.

Morphometric study of cartilage dynamics in the chick embryo tibia. II. Dexamethasone-treated embryos.

F B Ranz 1, J Aceitero 1, F Gaytan 1
PMCID: PMC1261837  PMID: 3446667

Abstract

The cartilage dynamics in the tibia of dexamethasone-treated chick embryos has been studied by means of morphometric methods. Treated embryos showed a delay in the longitudinal growth of the tibia, as well as in the growth of all structures enclosed by the perichondrium-periosteum. The cartilage formation rate remained nearly unchanged (above 1 mm3/day) from Day 12 to Day 14, whereas the cartilage resorption rate was zero up to Day 13, and showed a non-significant increase from Day 13 onwards. This might be related to the scarcity of resorptive cells found in the cartilage-marrow interface. By Day 14 a certain recovery of the growth rhythm was observed. These results indicate that the greatest effect of dexamethasone occurs at the level of cartilage resorption.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrett A. J., Sledge C. B., Dingle J. T. Effect of cortisol on the synthesis of chondroitin sulphate by embryonic cartilage. Nature. 1966 Jul 2;211(5044):83–84. doi: 10.1038/211083a0. [DOI] [PubMed] [Google Scholar]
  2. Blumenkrantz N., Asboe-Hansen G. Cortisol effect on collagen biosynthesis in embryonic explants and in vitro hydroxylation of protocollagen. Acta Endocrinol (Copenh) 1976 Nov;83(3):665–672. doi: 10.1530/acta.0.0830665. [DOI] [PubMed] [Google Scholar]
  3. Calcagno M., Goyena H., Arrambide E., Arruti de Urse C. Action of cortisone and cortisol upon biosynthesis of chondroitin sulfate in femur in vitro cultures of chick embryo. Exp Cell Res. 1970 Nov;63(1):131–137. doi: 10.1016/0014-4827(70)90340-x. [DOI] [PubMed] [Google Scholar]
  4. Cutroneo K. R., Rokowski R., Counts D. F. Glucocorticoids and collagen synthesis: comparison of in vivo and cell culture studies. Coll Relat Res. 1981 Nov;1(6):557–568. doi: 10.1016/s0174-173x(81)80037-4. [DOI] [PubMed] [Google Scholar]
  5. Dearden L. C., Mosier H. D., Jr Long-term recovery of chondrocytes in the tibial epiphyseal plate in rats after cortisone treatment. An electron microscopic study. Clin Orthop Relat Res. 1972 Sep;87:322–331. [PubMed] [Google Scholar]
  6. Ebert P. S., Prockop D. J. Influence of cortisol on the synthesis of sulfated mucopolysaccharides and collagen in chick embryos. Biochim Biophys Acta. 1967 Feb 7;136(1):45–55. doi: 10.1016/0304-4165(67)90319-4. [DOI] [PubMed] [Google Scholar]
  7. Gaytan F., Ranz F. B., Aceitero J. Morphometric study of cartilage dynamics in the chick embryo tibia. I. Methodology and tissue compartments in normal embryos. J Anat. 1987 Oct;154:63–71. [PMC free article] [PubMed] [Google Scholar]
  8. HUBLE J. Effects of cortisone-acetate on chondrogenesis and ossification in cockerels. Acta Endocrinol (Copenh) 1957 May;25(1):59–63. doi: 10.1530/acta.0.0250059. [DOI] [PubMed] [Google Scholar]
  9. HULTH A., OLERUD S. THE EFFECT OF CORTISONE ON GROWING BONE IN THE RAT. Br J Exp Pathol. 1963 Oct;44:491–496. [PMC free article] [PubMed] [Google Scholar]
  10. Hall B. K., Kalliecharan R. The effects of exogenous cortisone acetate on development (especially skeletal development) and on circulating levels of corticosteroids in chick embryos. Teratology. 1975 Oct;12(2):111–119. doi: 10.1002/tera.1420120204. [DOI] [PubMed] [Google Scholar]
  11. Jee W. S., Park H. Z., Roberts W. E., Kenner G. H. Corticosteroid and bone. Am J Anat. 1970 Dec;129(4):477–479. doi: 10.1002/aja.1001290409. [DOI] [PubMed] [Google Scholar]
  12. KARNOFSKY D. A., RIDGWAY L. P., PATTERSON P. A. Growth-inhibiting effect of cortisone acetate on the check embryo. Endocrinology. 1951 May;48(5):596–616. doi: 10.1210/endo-48-5-596. [DOI] [PubMed] [Google Scholar]
  13. KIVIRIKKO K. I. Effect of cortisone on collagen formation in the chick embryo. Nature. 1963 Jan 26;197:385–386. doi: 10.1038/197385b0. [DOI] [PubMed] [Google Scholar]
  14. LARON Z., BOSS J. H. Comparative effect of corticosterone and cortisone on rat bones. Arch Pathol. 1962 Apr;73:274–276. [PubMed] [Google Scholar]
  15. Lebovitz H. E., Eisenbarth G. S. Hormonal regulation of cartilage growth and metabolism. Vitam Horm. 1975;33:575–648. doi: 10.1016/s0083-6729(08)60973-5. [DOI] [PubMed] [Google Scholar]
  16. MOSCONA M. H., KARNOFSKY D. A. Cortisone induced modiffications in the development of the chick embryo. Endocrinology. 1960 Apr;66:533–549. doi: 10.1210/endo-66-4-533. [DOI] [PubMed] [Google Scholar]
  17. Rath N. C., Reddi A. H. Influence of adrenalectomy and dexamethasone on matrix-induced endochondral bone differentiation. Endocrinology. 1979 Jun;104(6):1698–1704. doi: 10.1210/endo-104-6-1698. [DOI] [PubMed] [Google Scholar]
  18. Reynolds J. J. The effect of hydrocortisone on the growth of chick bone rudiments in chemically defined medium. Exp Cell Res. 1966 Jan;41(1):174–189. doi: 10.1016/0014-4827(66)90557-x. [DOI] [PubMed] [Google Scholar]
  19. SOBEL H. Antagonistic effects of cortisone and growth-hormone on the developing chick embryo. Proc Soc Exp Biol Med. 1958 Mar;97(3):495–498. doi: 10.3181/00379727-97-23785. [DOI] [PubMed] [Google Scholar]
  20. Schryver H. F. The effect of hydrocortisone on chondroitin sulfate production and loss by embryonic chick tibiotarsi in organ culture. Exp Cell Res. 1965 Dec;40(3):610–618. doi: 10.1016/0014-4827(65)90238-7. [DOI] [PubMed] [Google Scholar]
  21. Silbermann M., Kadar T. Quantitative changes in the cellular population of the growth plate of triamcinolone-treated mice. Acta Anat (Basel) 1977;98(4):396–400. doi: 10.1159/000144818. [DOI] [PubMed] [Google Scholar]
  22. Silbermann M., Kleinhaus U., Livne E., Kedar T. Retardation of bone growth in triamcinolone-treated mice. J Anat. 1976 Jul;121(Pt 3):515–535. [PMC free article] [PubMed] [Google Scholar]
  23. Simmons D. J., Kunin A. S. Autoradiographic and biochemical investigations of the effect of cortisone on the bones of the rat. Clin Orthop Relat Res. 1967 Nov-Dec;55:201–215. [PubMed] [Google Scholar]
  24. Takano T., Takigawa M., Suzuki F. Stimulation by glucocorticoids of the differentiated phenotype of chondrocytes and the proliferation of rabbit costal chondrocytes in culture. J Biochem. 1985 Apr;97(4):1093–1100. doi: 10.1093/oxfordjournals.jbchem.a135153. [DOI] [PubMed] [Google Scholar]
  25. Tapp E. The effects of hormones on bone in growing rats. J Bone Joint Surg Br. 1966 Aug;48(3):526–531. [PubMed] [Google Scholar]

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