Abstract
A model is proposed, based on recent peptide analog and ice crystal etching studies, whereby an alanine-rich, alpha-helical antifreeze polypeptide (AFP) from the winter flounder inhibits the growth of ice crystals by hydrogen bonding of Thr, Asn, and Asp side chains in a specific pattern to the [2021] hexagonal bipyramidal planes of ice. It is further suggested that this mode of binding is unidirectional, maximizing opportunities for packing of AFPs on the ice surface, and that ice crystal growth inhibition occurs by a two-step mechanism involving hydrogen bonding and hydrophobic interpeptide interactions.
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Selected References
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- Chou K. C. Energy-optimized structure of antifreeze protein and its binding mechanism. J Mol Biol. 1992 Jan 20;223(2):509–517. doi: 10.1016/0022-2836(92)90666-8. [DOI] [PubMed] [Google Scholar]
- Davies P. L., Hew C. L. Biochemistry of fish antifreeze proteins. FASEB J. 1990 May;4(8):2460–2468. doi: 10.1096/fasebj.4.8.2185972. [DOI] [PubMed] [Google Scholar]
- Feeney R. E., Burcham T. S., Yeh Y. Antifreeze glycoproteins from polar fish blood. Annu Rev Biophys Biophys Chem. 1986;15:59–78. doi: 10.1146/annurev.bb.15.060186.000423. [DOI] [PubMed] [Google Scholar]
- Knight C. A., Cheng C. C., DeVries A. L. Adsorption of alpha-helical antifreeze peptides on specific ice crystal surface planes. Biophys J. 1991 Feb;59(2):409–418. doi: 10.1016/S0006-3495(91)82234-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raymond J. A., DeVries A. L. Adsorption inhibition as a mechanism of freezing resistance in polar fishes. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2589–2593. doi: 10.1073/pnas.74.6.2589. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wen D., Laursen R. A. Structure-function relationships in an antifreeze polypeptide. The role of neutral, polar amino acids. J Biol Chem. 1992 Jul 15;267(20):14102–14108. [PubMed] [Google Scholar]
- Yang D. S., Sax M., Chakrabartty A., Hew C. L. Crystal structure of an antifreeze polypeptide and its mechanistic implications. Nature. 1988 May 19;333(6170):232–237. doi: 10.1038/333232a0. [DOI] [PubMed] [Google Scholar]