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. 1993 Mar;64(3):735–742. doi: 10.1016/S0006-3495(93)81433-4

Imaging viscoelasticity by force modulation with the atomic force microscope

M Radmacher 1, R W Tillmann 1, H E Gaub 1
PMCID: PMC1262386  PMID: 19431876

Abstract

Force modulation and phase sensitive detection was used to image soft surfaces with the atomic force microscope. This force modulation microscopy allows the simultaneous recording of images of the surface profile, the storage modulus, and the loss modulus of the sample. A theoretical treatment of the elastic tip-sample interaction is given. As examples, images of Langmuir-Blodgett films of a polymeric amphiphile and of a structured fatty acid are presented.

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Selected References

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  1. Binnig G, Quate CF, Gerber C. Atomic force microscope. Phys Rev Lett. 1986 Mar 3;56(9):930–933. doi: 10.1103/PhysRevLett.56.930. [DOI] [PubMed] [Google Scholar]
  2. Drake B., Prater C. B., Weisenhorn A. L., Gould S. A., Albrecht T. R., Quate C. F., Cannell D. S., Hansma H. G., Hansma P. K. Imaging crystals, polymers, and processes in water with the atomic force microscope. Science. 1989 Mar 24;243(4898):1586–1589. doi: 10.1126/science.2928794. [DOI] [PubMed] [Google Scholar]
  3. Engel A. Biological applications of scanning probe microscopes. Annu Rev Biophys Biophys Chem. 1991;20:79–108. doi: 10.1146/annurev.bb.20.060191.000455. [DOI] [PubMed] [Google Scholar]
  4. Heyn S. P., Tillmann R. W., Egger M., Gaub H. E. A miniaturized micro-fluorescence film balance for protein-containing lipid monolayers spread from a vesicle suspension. J Biochem Biophys Methods. 1991 Feb-Mar;22(2):145–158. doi: 10.1016/0165-022x(91)90027-t. [DOI] [PubMed] [Google Scholar]
  5. Radmacher M., Tillamnn R. W., Fritz M., Gaub H. E. From molecules to cells: imaging soft samples with the atomic force microscope. Science. 1992 Sep 25;257(5078):1900–1905. doi: 10.1126/science.1411505. [DOI] [PubMed] [Google Scholar]
  6. Weisenhorn A. L., Drake B., Prater C. B., Gould S. A., Hansma P. K., Ohnesorge F., Egger M., Heyn S. P., Gaub H. E. Immobilized proteins in buffer imaged at molecular resolution by atomic force microscopy. Biophys J. 1990 Nov;58(5):1251–1258. doi: 10.1016/S0006-3495(90)82465-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Zanoni R, Naselli C, Bell J, Stegeman GI, Seaton CT. Elastic properties of Langmuir-Blodgett films. Phys Rev Lett. 1986 Dec 1;57(22):2838–2840. doi: 10.1103/PhysRevLett.57.2838. [DOI] [PubMed] [Google Scholar]

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