Abstract
Two sensitive serum neutralization (SN) tests for the detection of antibodies to bovine herpesvirus-1 (BHV-1) in bovine sera were evaluated. Both SN tests used a 24 h incubation of test sera with 100 CCID50 of BHV-1 before the addition of susceptible cells. The tests differed in the presence (C test) or absence (D test) of complement and were compared with a standard 1 h incubation SN test and the enzyme-linked immunosorbent assay (ELISA). Although the mean titer of the C test was twofold higher than the mean titer of the D test for 310 sera, the number of samples which were negative was not significantly different between tests. For 100 sera from herds with known reactors, which were negative in a 1 h incubation SN test, 32% tested positive in the C and D tests. Other investigations, including Western immunoblotting and radioimmune precipitation, suggest that the 24 h incubation tests produce some false positive results. In contrast, the 1 h incubation SN test and, to a much lesser extent, the ELISA appear to produce some false negative results. The C test was more sensitive than the D test for detecting an early immune response after experimental infection.
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- Bitsch V. The P 37/24 modification of the infectious bovine rhinotracheitis virus-serum neutralization test. Acta Vet Scand. 1978;19(4):497–505. doi: 10.1186/BF03547589. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bradley J. A. Eradication of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) from a herd of beef cattle. Can Vet J. 1985 Jun;26(6):195–198. [PMC free article] [PubMed] [Google Scholar]
- Cho H. J., Bohac J. G. Sensitivity and specificity of an enzyme-linked immunosorbent assay for the detection of infectious bovine rhinotracheitis viral antibody in cattle. Can J Comp Med. 1985 Apr;49(2):189–194. [PMC free article] [PubMed] [Google Scholar]
- Deregt D., Sabara M., Babiuk L. A. Structural proteins of bovine coronavirus and their intracellular processing. J Gen Virol. 1987 Nov;68(Pt 11):2863–2877. doi: 10.1099/0022-1317-68-11-2863. [DOI] [PubMed] [Google Scholar]
- Misra V., Blumenthal R. M., Babiuk L. A. Proteins Specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus). J Virol. 1981 Nov;40(2):367–378. doi: 10.1128/jvi.40.2.367-378.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pastoret P. P., Babiuk L. A., Misra V., Griebel P. Reactivation of temperature-sensitive and non-temperature-sensitive infectious bovine rhinotracheitis vaccine virus with dexamethasone. Infect Immun. 1980 Aug;29(2):483–488. doi: 10.1128/iai.29.2.483-488.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Potgieter L. N. The influence of complement on the neutralization of infectious bovine rhinotracheitis virus by globulins derived from early and late bovine antisera. Can J Comp Med. 1975 Oct;39(4):427–433. [PMC free article] [PubMed] [Google Scholar]
- Rossi C. R., Kiesel G. K. Antibody class and complement requirement of neutralizing antibodies in the primary and secondary antibody response of cattle to infectious bovine rhinotracheitis virus vaccine. Arch Virol. 1976;51(3):191–198. doi: 10.1007/BF01318023. [DOI] [PubMed] [Google Scholar]
- van Drunen Littel-van den Hurk S., Babiuk L. A. Polypeptide specificity of the antibody response after primary and recurrent infection with bovine herpesvirus 1. J Clin Microbiol. 1986 Feb;23(2):274–282. doi: 10.1128/jcm.23.2.274-282.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Drunen Littel-van den Hurk S., van den Hurk J. V., Gilchrist J. E., Misra V., Babiuk L. A. Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: characterization of their biochemical and immunological properties. Virology. 1984 Jun;135(2):466–479. doi: 10.1016/0042-6822(84)90201-0. [DOI] [PubMed] [Google Scholar]