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. 1995 Jul;59(3):205–212.

Assessment of glycosaminoglycan concentration in equine synovial fluid as a marker of joint disease.

J L Palmer 1, A L Bertone 1, H McClain 1
PMCID: PMC1263767  PMID: 8521354

Abstract

A modification of a colorimetric assay was used to determine synovial fluid total and individual sulphated-glycosaminoglycan concentration in various clinical presentations of joint disease in horses. Concentrations of synovial fluid and serum sulphated-glycosaminoglycan (GAG) were measured by the 1,9-dimethylmethylene blue (DMMB) dye assay in normal horses (n = 49), horses with acute (n = 26) or chronic (n = 27) joint disease (defined by clinical, radiographic, and clinicopathological parameters), and horses with cartilaginous lesions at diagnostic arthroscopy, but with normal radiographs and synovial fluid (n = 9). Horses with acute joint disease were subdivided into moderate acute (n = 21) and severe acute (n = 5) joint disease on the basis of synovial fluid analysis and clinical examination. Horses with chronic joint disease were subdivided into mild chronic (n = 9), moderate chronic (n = 10), and severe chronic (n = 8) joint disease on the basis of synovial fluid analysis, clinical examination, and radiographic findings. The concentrations of chondroitin sulphate (CS) and keratan sulphate (KS) were analyzed in each sample following sequential enzymatic digestion of the sample with chondroitinase or keratanase. In addition, the concentration of hyaluronate (HA) in each sample was determined by a colorimetric assay following digestion of the sample with microbial hyaluronidase.(ABSTRACT TRUNCATED AT 250 WORDS)

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwan W. H., Carter S. D., Bennett D., Edwards G. B. Glycosaminoglycans in horses with osteoarthritis. Equine Vet J. 1991 Jan;23(1):44–47. doi: 10.1111/j.2042-3306.1991.tb02712.x. [DOI] [PubMed] [Google Scholar]
  2. Alwan W. H., Carter S. D., Bennett D., May S. A., Edwards G. B. Cartilage breakdown in equine osteoarthritis: measurement of keratan sulphate by an ELISA system. Res Vet Sci. 1990 Jul;49(1):56–60. [PubMed] [Google Scholar]
  3. Brandt K. D. A pessimistic view of serologic markers for diagnosis and management of osteoarthritis. Biochemical, immunologic and clinicopathologic barriers. J Rheumatol Suppl. 1989 Aug;18:39–42. [PubMed] [Google Scholar]
  4. Castor C. W., Prince R. K., Hazelton M. J. Hyaluronic acid in human synovial effusions; a sensitive indicator of altered connective tissue cell function during inflammation. Arthritis Rheum. 1966 Dec;9(6):783–794. doi: 10.1002/art.1780090606. [DOI] [PubMed] [Google Scholar]
  5. Caterson B. Immunological aspects of markers of joint disease. J Rheumatol Suppl. 1991 Feb;27:19–23. [PubMed] [Google Scholar]
  6. Farndale R. W., Buttle D. J., Barrett A. J. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta. 1986 Sep 4;883(2):173–177. doi: 10.1016/0304-4165(86)90306-5. [DOI] [PubMed] [Google Scholar]
  7. Fubini S. L., Boatwright C. E., Todhunter R. J., Lust G. Effect of intramuscularly administered polysulfated glycosaminoglycan on articular cartilage from equine joints injected with methylprednisolone acetate. Am J Vet Res. 1993 Aug;54(8):1359–1365. [PubMed] [Google Scholar]
  8. Hardingham T., Bayliss M. Proteoglycans of articular cartilage: changes in aging and in joint disease. Semin Arthritis Rheum. 1990 Dec;20(3 Suppl 1):12–33. doi: 10.1016/0049-0172(90)90044-g. [DOI] [PubMed] [Google Scholar]
  9. Hilbert B. J., Rowley G., Antonas K. N. Hyaluronic acid concentration in synovial fluid from normal and arthritic joints of horses. Aust Vet J. 1984 Jan;61(1):22–24. doi: 10.1111/j.1751-0813.1984.tb07125.x. [DOI] [PubMed] [Google Scholar]
  10. Jourdian G. W., Wolfman M., Sarber R., Distler J. A specific, sensitive method for the determination of hyaluronate. Anal Biochem. 1979 Jul 15;96(2):474–480. doi: 10.1016/0003-2697(79)90609-2. [DOI] [PubMed] [Google Scholar]
  11. Little C. B., Hilbert B. J., Wickstrom S., Hedlund B. E. Quantitative microanalysis of equine synovial fluid glycosaminoglycan concentration. Am J Vet Res. 1990 Oct;51(10):1534–1539. [PubMed] [Google Scholar]
  12. Mankin H. J., Lippiello L. The glycosaminoglycans of normal and arthritic cartilage. J Clin Invest. 1971 Aug;50(8):1712–1719. doi: 10.1172/JCI106660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mehraban F., Finegan C. K., Moskowitz R. W. Serum keratan sulfate. Quantitative and qualitative comparisons in inflammatory versus noninflammatory arthritides. Arthritis Rheum. 1991 Apr;34(4):383–392. doi: 10.1002/art.1780340403. [DOI] [PubMed] [Google Scholar]
  14. Nilsson G., Persson L. Changes in the synovial fluid of equine fetlock joints with lesions in the cartilage and joint capsule. A study in the standardbred horse. Acta Vet Scand Suppl. 1973;44(0):99–107. [PubMed] [Google Scholar]
  15. Rowley G., Antonas K. N., Hilbert B. J. Quantitation of hyaluronic acid in equine synovia. Am J Vet Res. 1982 Jun;43(6):1096–1099. [PubMed] [Google Scholar]
  16. Saxne T., Heinegård D., Wollheim F. A. Cartilage proteoglycans in synovial fluid and serum in patients with inflammatory joint disease. Relation to systemic treatment. Arthritis Rheum. 1987 Sep;30(9):972–979. doi: 10.1002/art.1780300903. [DOI] [PubMed] [Google Scholar]
  17. Todhunter R. J., Yeager A. E., Freeman K. P., Parente E. J., Lust G. Keratan sulfate as a marker of articular cartilage catabolism and joint treatment in ponies. Am J Vet Res. 1993 Jul;54(7):1007–1016. [PubMed] [Google Scholar]
  18. Tulamo R. M. Comparison of high-performance liquid chromatography with a radiometric assay for determination of the effect of intra-articular administration of corticosteroid and saline solution on synovial fluid hyaluronate concentration in horses. Am J Vet Res. 1991 Dec;52(12):1940–1944. [PubMed] [Google Scholar]

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