Skip to main content
PMC home page
Canadian Journal of Veterinary Research logoLink to Canadian Journal of Veterinary Research
. 1996 Jan;60(1):50–54.

Comparison of Newcastle disease viruses isolated from cormorants in Canada and the USA in 1975, 1990 and 1992.

R A Heckert 1, M S Collins 1, R J Manvell 1, I Strong 1, J E Pearson 1, D J Alexander 1
PMCID: PMC1263800  PMID: 8825994

Abstract

Seventeen Newcastle disease virus (NDV) isolates obtained from cormorants, turkeys, a pelican, and a gull in Canada and the USA collected in 1975, 1990 and 1992 were analyzed for relatedness by monoclonal antibody profiling. In addition, nucleotide sequence analysis was performed in two areas of the fusion (F) gene for 5 of the isolates. No difference in the antigenicity of these 17 viruses, as determined by monoclonal antibody binding patterns, was seen. The amino acid sequences obtained via nucleotide sequencing at the cleavage site of the F protein showed that all the isolates tested had two pairs of basic amino acids immediately upstream of the cleavage site, and a phenylalanine residue at the N-terminus of the F1 protein, which is consistent with velogenic NDV. The deduced amino acid sequence obtained at the cleavage site of the F protein from 6 of the isolates was virtually identical regardless of the species, year of isolation, or location. However, the 1975 cormorant isolate showed marked differences from the 1990-1992 isolates in the nucleotide and deduced amino acid sequence of the F gene signal region. These data indicate that the 1990 and 1992 outbreaks were caused by the same epizootic virus and further suggest that the population of NDV in these wild birds may be very stable. The belief that the velogenic NDV circulating in cormorants in 1992 was transmitted into the free-ranging turkey flocks located near the cormorants in North Dakota is supported by the present study in which no distinction could be made between the viruses isolated from turkeys or wild birds.

Full text

PDF
51

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Collins M. S., Alexander D. J., Brockman S., Kemp P. A., Manvell R. J. Evaluation of mouse monoclonal antibodies raised against an isolate of the variant avian paramyxovirus type 1 responsible for the current panzootic in pigeons. Arch Virol. 1989;104(1-2):53–61. doi: 10.1007/BF01313807. [DOI] [PubMed] [Google Scholar]
  2. Collins M. S., Bashiruddin J. B., Alexander D. J. Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. Arch Virol. 1993;128(3-4):363–370. doi: 10.1007/BF01309446. [DOI] [PubMed] [Google Scholar]
  3. Collins M. S., Strong I., Alexander D. J. Evaluation of the molecular basis of pathogenicity of the variant Newcastle disease viruses termed "pigeon PMV-1 viruses". Arch Virol. 1994;134(3-4):403–411. doi: 10.1007/BF01310577. [DOI] [PubMed] [Google Scholar]
  4. Gotoh B., Sakaguchi T., Nishikawa K., Inocencio N. M., Hamaguchi M., Toyoda T., Nagai Y. Structural features unique to each of the three antigenic sites on the hemagglutinin-neuraminidase protein of Newcastle disease virus. Virology. 1988 Mar;163(1):174–182. doi: 10.1016/0042-6822(88)90244-9. [DOI] [PubMed] [Google Scholar]
  5. Heckert R. A. Ontario. Newcastle disease in cormorants. Can Vet J. 1993 Mar;34(3):184–184. [PMC free article] [PubMed] [Google Scholar]
  6. Macpherson L. W. Some Observations On The Epizootiology Of NewCastle Disease. Can J Comp Med Vet Sci. 1956 May;20(5):155–168. [PMC free article] [PubMed] [Google Scholar]
  7. Nagai Y., Hamaguchi M., Toyoda T. Molecular biology of Newcastle disease virus. Prog Vet Microbiol Immunol. 1989;5:16–64. [PubMed] [Google Scholar]
  8. Russell P. H., Alexander D. J. Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodies. Arch Virol. 1983;75(4):243–253. doi: 10.1007/BF01314890. [DOI] [PubMed] [Google Scholar]
  9. Schuy W., Garten W., Linder D., Klenk H. D. The carboxyterminus of the hemagglutinin-neuraminidase of Newcastle disease virus is exposed at the surface of the viral envelope. Virus Res. 1984;1(5):415–426. doi: 10.1016/0168-1702(84)90027-3. [DOI] [PubMed] [Google Scholar]
  10. Toyoda T., Sakaguchi T., Hirota H., Gotoh B., Kuma K., Miyata T., Nagai Y. Newcastle disease virus evolution. II. Lack of gene recombination in generating virulent and avirulent strains. Virology. 1989 Apr;169(2):273–282. doi: 10.1016/0042-6822(89)90152-9. [DOI] [PubMed] [Google Scholar]
  11. Wernery U., Remple J. D., Neumann U., Alexander D. J., Manvell R. J., Kaaden O. R. Avian paramyxovirus serotype 1 (Newcastle disease virus)--infections in falcons. Zentralbl Veterinarmed B. 1992 May;39(3):153–158. doi: 10.1111/j.1439-0450.1992.tb01152.x. [DOI] [PubMed] [Google Scholar]
  12. Wobeser G., Leighton F. A., Norman R., Myers D. J., Onderka D., Pybus M. J., Neufeld J. L., Fox G. A., Alexander D. J. Newcastle disease in wild water birds in western Canada, 1990. Can Vet J. 1993 Jun;34(6):353–359. [PMC free article] [PubMed] [Google Scholar]

Articles from Canadian Journal of Veterinary Research are provided here courtesy of Canadian Veterinary Medical Association

RESOURCES