Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1965 Oct;97(1):32–36. doi: 10.1042/bj0970032

Depletion of glycogen synthetase and increase of glucose 6-phosphate dehydrogenase in livers of ethionine-treated mice

Hsien-Gieh Sie 1, Ann Hablanian 1
PMCID: PMC1264540  PMID: 16749121

Abstract

1. Ethionine-treated mice showed a marked depletion in liver glycogen, a decrease of glycogen-synthetase activity, an increase in activity of glucose 6-phosphate dehydrogenase and the solubilization of phosphorylase. 2. The administration of cortisol or glucose did not alleviate these changes but the effect of ethionine was completely prevented in animals given methionine as well as ethionine. 3. The activities of the following enzymes were unchanged: hexokinase, glucokinase, glucose 6-phosphatase, phosphoglucomutase, 6-phosphogluconate dehydrogenase, UDP-glucose pyrophosphorylase, UDP-glucose dehydrogenase and pyruvate kinase.

Full text

PDF
32

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTELS H., HOHORST H. J. [On the effect of ethionine on the metabolite status of the rat liver]. Biochim Biophys Acta. 1963 Apr 2;71:214–216. doi: 10.1016/0006-3002(63)91011-4. [DOI] [PubMed] [Google Scholar]
  2. CHATAGNER F. OCCURRENCE OF HOMOSERINE IN THE LIVER OF ETHIONINE- OR METHIONINE-TREATED RATS. Nature. 1964 Sep 12;203:1177–1178. doi: 10.1038/2031177b0. [DOI] [PubMed] [Google Scholar]
  3. CORI G. T., CORI C. F. Glucose-6-phosphatase of the liver in glycogen storage disease. J Biol Chem. 1952 Dec;199(2):661–667. [PubMed] [Google Scholar]
  4. CRANE R. K., SOLS A. The association of hexokinase with particulate fractions of brain and other tissue homogenates. J Biol Chem. 1953 Jul;203(1):273–292. [PubMed] [Google Scholar]
  5. DISCHE Z. Spectrophotometric method for the determination of free pentose and pentose in nucleotides. J Biol Chem. 1949 Nov;181(1):379–392. [PubMed] [Google Scholar]
  6. FITCH W. M., HILL R., CHAIKOFF I. L. The effect of fructose feeding on glycolytic enzyme activities of the normal rat liver. J Biol Chem. 1959 May;234(5):1048–1051. [PubMed] [Google Scholar]
  7. GLOCK G. E., McLEAN P. Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J. 1953 Oct;55(3):400–408. doi: 10.1042/bj0550400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HOFERT J. F., BOUTWELL R. K. PUROMYCIN-INDUCED GLYCOGENOLYSIS AS AN EVEN INDEPENDENT FROM INHIBITED PROTEIN SYNTHESIS IN MOUSE LIVER; EFFECTS OF PUROMYCIN ANALOGS. Arch Biochem Biophys. 1963 Dec;103:338–344. doi: 10.1016/0003-9861(63)90423-5. [DOI] [PubMed] [Google Scholar]
  9. LELOIR L. F., GOLDEMBERG S. H. Synthesis of glycogen from uridine diphosphate glucose in liver. J Biol Chem. 1960 Apr;235:919–923. [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. LUPU C. I., FARBER E. Effects of ethionine upon hepatic glycogen formation from glucose in intact rats. Proc Soc Exp Biol Med. 1954 Aug-Sep;86(4):701–705. doi: 10.3181/00379727-86-21206. [DOI] [PubMed] [Google Scholar]
  12. Marks P. A., Banks J. INHIBITION OF MAMMALIAN GLUCOSE-6-PHOSPHATE DEHYDROGENASE BY STEROIDS. Proc Natl Acad Sci U S A. 1960 Apr;46(4):447–452. doi: 10.1073/pnas.46.4.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NIGAM V. N., MACDONALD H. L., CANTERO A. Limiting factors for glycogen storage in tumors. I. Limiting enzymes. Cancer Res. 1962 Feb;22:131–138. [PubMed] [Google Scholar]
  14. SHULL K. H. Hepatic phosphorylase and adenosine triphosphate levels in ethionine-treated rats. J Biol Chem. 1962 May;237:1734–1735. [PubMed] [Google Scholar]
  15. SIE H. G., FISHMAN W. H. GLYCOGEN SYNTHETASE: ITS RESPONSE TO CORTISOL. Science. 1964 Feb 21;143(3608):816–817. doi: 10.1126/science.143.3608.816. [DOI] [PubMed] [Google Scholar]
  16. SIE H. G., HABLANIAN A., FISHMAN W. H. SOLUBILIZATION OF MOUSE LIVER GLYCOGEN SYNTHETASE AND PHOSPHORYLASE DURING STARVATION GLYCOGENOLYSIS AND ITS REVERSAL BY CORTISOL. Nature. 1964 Jan 25;201:393–394. doi: 10.1038/201393a0. [DOI] [PubMed] [Google Scholar]
  17. SPAIN J. D., GRIFFIN A. C. A histochemical study of glycogen alterations in the livers of rats following azo dye administration. Cancer Res. 1957 Apr;17(3):200–204. [PubMed] [Google Scholar]
  18. STEKOL J. A., BEDRAK E., MODY U., BURNETTE N., SOMERVILLE C. Inhibition of the synthesis of diphosphopyridine nucleotide by ethionine administration in the liver of rats and mice. J Biol Chem. 1963 Jan;238:469–473. [PubMed] [Google Scholar]
  19. TURNER M. K., REID E. AN ANOMALOUS EFFECT OF ETHIONINE ON RIBONUCLEIC ACID SYNTHESIS. Nature. 1964 Sep 12;203:1174–1175. doi: 10.1038/2031174a0. [DOI] [PubMed] [Google Scholar]
  20. Tata J. R. Subcellular redistribution of a liver alpha-glucan phosphorylase during alterations in glycogen content. Biochem J. 1964 Feb;90(2):284–292. doi: 10.1042/bj0900284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. VILLA-TREVINO S., FARBER E., STAEHELIN T., WETTSTEIN F. O., NOLL H. BREAKDOWN AND REASSEMBLY OF RAT LIVER ERGOSOMES AFTER ADMINISTRATION OF ETHIONINE OR PUROMYCIN. J Biol Chem. 1964 Nov;239:3826–3833. [PubMed] [Google Scholar]
  22. WEBER G., SINGHAL R. L., STAMM N. B. ACTINOMYCIN: INHIBITION OF CORTISONE-INDUCED SYNTHESIS OF HEPATIC GLUCONEOGENIC ENZYMES. Science. 1963 Oct 18;142(3590):390–392. doi: 10.1126/science.142.3590.390. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES