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. 1965 Oct;97(1):176–179. doi: 10.1042/bj0970176

The assimilation of acetate and propionate by Prototheca zopfi

D Lloyd 1, A G Callely 1
PMCID: PMC1264558  PMID: 16749100

Abstract

1. The tricarboxylic acid and glyoxylate cycles are of major importance in the assimilation of acetate and propionate by Prototheca zopfii. The pattern of assimilation of [2-14C]acetate and [2-14C]propionate by whole cells growing with their respective substrates is similar except that, with propionate, β-hydroxypropionate is the first labelled intermediate detected. 2. Carbon dioxide fixation is of little quantitative importance for the growth of this organism with propionate. 3. The yield of cells obtained/mole of acetate is similar to that obtained/mole of propionate and about half that obtained/mole of n-butyrate, these substrates acting as sole sources of carbon and energy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARNSTEIN H. R., WHITE A. M. The function of vitamin B12 in the metabolism of propionate by the protozoan Ochromonas malhamensis. Biochem J. 1962 May;83:264–270. doi: 10.1042/bj0830264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CASSELTON P. J. An effect of yeast extract on the growth of Chlorella. Nature. 1959 May 16;183(4672):1404–1404. doi: 10.1038/1831404a0. [DOI] [PubMed] [Google Scholar]
  3. CIFERRI O., SALA F. Carbohydrate metabolism of Prototheca zopfil. II. Carbon dioxide fixation by cell-free extracts. Enzymologia. 1962 Jun 30;24:298–308. [PubMed] [Google Scholar]
  4. Callely A. G., Lloyd D. The metabolism of acetate in the colourless alga, Prototheca zopfii. Biochem J. 1964 Mar;90(3):483–489. doi: 10.1042/bj0900483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Callely A. G., Lloyd D. The metabolism of propionate in the colourless alga, Prototheca zopfii. Biochem J. 1964 Aug;92(2):338–345. doi: 10.1042/bj0920338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KORNBERG H. L., DAVIES R. E., WOOD D. R. The metabolism of 14C-labelled bicarbonate in the cat. Biochem J. 1952 Jun;51(3):351–357. doi: 10.1042/bj0510351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KORNBERG H. L., MADSEN N. B. The metabolism of C2 compounds in microorganisms. 3. Synthesis of malate from acetate via the glyoxylate cycle. Biochem J. 1958 Mar;68(3):549–557. doi: 10.1042/bj0680549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KORNBERG H. L., QUAYLE J. R. The metabolism of C2 compounds in microorganisms. 2. The effect of carbon dioxide on the incorporation of [14C] acetate by acetate-grown Pseudomonas KB1. Biochem J. 1958 Mar;68(3):542–549. doi: 10.1042/bj0680542. [DOI] [PMC free article] [PubMed] [Google Scholar]

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