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. 1965 Nov;97(2):513–517. doi: 10.1042/bj0970513

Lactate dehydrogenase isoenzymes of human semen

Jørgen Clausen 1, Bjarni Øvlisen 1
PMCID: PMC1264668  PMID: 16749157

Abstract

1. A lactate dehydrogenase isoenzyme present in human spermatozoa and semen was isolated and characterized biochemically in term of its pH for optimum activity and by means of Km values for lactate, NAD+ and NAD analogues. The results were compared with those obtained with the human heart-type and the liver-type lactate dehydrogenase isoenzymes. 2. The enzyme was characterized by its resistance to digestion with different proteolytic enzymes. The time for 50% digestion in terms of residual dehydrogenase activity was compared with times obtained for the H4- and M4-types.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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