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. 1965 Nov;97(2):595–606. doi: 10.1042/bj0970595

Further observations on the polynucleotide-induced stimulation of protein synthesis by cell-free preparations from rabbit reticulocytes

K G Nair 1,*, H R V Arnstein 1
PMCID: PMC1264681  PMID: 16749170

Abstract

1. The effect of high-molecular-weight RNA from reticulocyte polyribosomes (messenger RNA) on protein synthesis by subcellular fractions derived from reticulocytes, reported by Arnstein, Cox & Hunt (1964), has now been studied in detail. Optimum response of the cell-free system requires 30–50mm-K+ and approx. 5mm-Mg2+ in the pH range 7·4–7·6. 2. RNA stimulates the incorporation into protein of both free amino acids and of aminoacyl residues from s-RNA. Stimulation by either RNA or polyuridylic acid is dependent on a labile factor or enzyme, which is present in the `pH5 fraction' and may be concerned with the formation of new polysomes. Quantitatively the response of the cell-free system to RNA is similar to that of polyuridylic acid, and there appears to be competition between messenger RNA and polyuridylic acid or polyadenylic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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