Abstract
1. An exopolygalacturonase was separated from a mycelial extract of Aspergillus niger with a 290-fold purification and a recovery of 8·6%. 2. The enzyme displayed its full activity only in the presence of Hg2+ ions; KA for mercuric chloride was about 6×10−8m. 3. The mercury-activated enzyme progressively removed the terminal galacturonic acid residues from α-(1→4)-linked galacturonide chains and converted digalacturonic acid, trigalacturonic acid, tetragalacturonic acid and pectic acid into galacturonic acid.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AYRES A., DINGLE J., PHIPPS A., REID W. W., SOLOMONS G. L. Enzymic degradation of pectic acid and the complex nature of polygalacturonase. Nature. 1952 Nov 15;170(4333):834–836. doi: 10.1038/170834a0. [DOI] [PubMed] [Google Scholar]
- LUH B. S., PHAFF H. J. The preparation of pure di- and trigalacturonic acids. Arch Biochem Biophys. 1952 Mar;36(1):231–232. doi: 10.1016/0003-9861(52)90393-7. [DOI] [PubMed] [Google Scholar]
- Mill P. J. The pectic enzymes of Aspergillus niger. A second exopolygalacturonase. Biochem J. 1966 Jun;99(3):562–565. doi: 10.1042/bj0990562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TUTTOBELLO R., MILL P. J. The pectic enzymes of Aspergillus niger. 1. The production of active mixtures of pectic enzymes. Biochem J. 1961 Apr;79:51–57. doi: 10.1042/bj0790051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TUTTOBELLO R., MILL P. J. The pectic enzymes of Aspergillus niger. 1. The production of active mixtures of pectic enzymes. Biochem J. 1961 Apr;79:51–57. doi: 10.1042/bj0790051. [DOI] [PMC free article] [PubMed] [Google Scholar]