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. 1966 Jul;100(1):197–203. doi: 10.1042/bj1000197

Studies of the congenitally goitrous sheep. Composition and metabolism of goitrous thyroid tissue

Ian R Falconer 1
PMCID: PMC1265110  PMID: 6007447

Abstract

1. Normal and congenitally goitrous thyroid tissue was examined to identify the defective mechanism in the goitrous glands. 2. The uptake of [131I]iodide from the blood stream into the goitrous glands (average 74·9%) was significantly greater than normal (average 43·5%; P < 0·005), as was the rate of [131I]iodide release (goitrous t½ average 72·3hr., normal t½ average 198·7hr.; P0·025). 3. The l-[131I]-iodotyrosine-deiodinase activity was significantly (P0·02) greater than normal in goitrous-thyroid slices. 4. The 0·9%-sodium chloride-soluble proteins of [131I]-iodide-labelled thyroid glands were fractionated with ammonium sulphate: 68·7±4·0% of the total radioactivity appeared in the 35–45%-saturation precipitate from normal gland extracts, but less than 20% of the total radioactivity was in in this fraction from goitre extracts. 5. Ultracentrifugal analysis of 0·9%-sodium chloride-soluble proteins of goitrous glands showed no protein of S20,w 19–20s (thyroglobulin) even when the animals had previously received 0·1–2·0mg. of l-thyroxine/day intramuscularly for 40 days. The major proteins of goitrous glands had S20,w 3·2–7·6s. 6. The incorporation in incubated slices of [14C]proline and [14C]leucine into soluble proteins precipitated by 35–42%-saturated ammonium sulphate was markedly lower in goitrous tissue. 7. It was concluded that the goitrous tissue exhibited defective biosynthesis of thyroglobulin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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