Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1966 Sep;100(3):793–800. doi: 10.1042/bj1000793

Some properties of rat-liver glucose–adenosine triphosphate phosphotransferases

Patricia McLean 1, J Brown 1
PMCID: PMC1265217  PMID: 5969293

Abstract

In normal rat liver hexokinase (EC 2.7.1.1) activity usually accounts for not more than 30% of the total glucose–ATP phosphotransferase activity, the remainder being due to glucokinase (EC 2.7.1.2). In the present work it was found that in normal rat liver the relative activities of these two enzymes were occasionally very different from those usually found even though the total glucose–ATP phosphotransferase activity was within the normal range. In some cases almost the entire glucose–ATP phosphotransferase was accounted for by the low-Km enzyme hexokinase. Some properties of this enzyme system are reported. It is suggested that this shift in favour of the low-Km enzyme without change in the total glucose–ATP phosphotransferase activity may represent a regulatory mechanism.

Full text

PDF
795

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLUMENTHAL M. D., ABRAHAM S., CHAIKOFF I. L. ADAPTIVE BEHAVIOR OF HEPATIC GLUCOKINASE IN THE ALLOXAN-DIABETIC RAT. Arch Biochem Biophys. 1964 Feb;104:225–230. doi: 10.1016/s0003-9861(64)80007-2. [DOI] [PubMed] [Google Scholar]
  2. Ballard F. J., Oliver I. T. Ketohexokinase, isoenzymes of glucokinase and glycogen synthesis from hexoses in neonatal rat liver. Biochem J. 1964 Feb;90(2):261–268. doi: 10.1042/bj0900261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GLOCK G. E., McLEAN P. Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J. 1953 Oct;55(3):400–408. doi: 10.1042/bj0550400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. González C., Ureta T., Sánchez R., Niemeyer H. Multiple molecular forms of ATP:hexose 6-phosphotransferase from rat liver. Biochem Biophys Res Commun. 1964 Jul 1;16(4):347–352. doi: 10.1016/0006-291x(64)90038-5. [DOI] [PubMed] [Google Scholar]
  5. Katzen H. M., Schimke R. T. Multiple forms of hexokinase in the rat: tissue distribution, age dependency, and properties. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1218–1225. doi: 10.1073/pnas.54.4.1218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McLean P., Brown J. Activities of some enzymes concerned with citrate and glucose metabolism in transplanted rat hepatomas. Biochem J. 1966 Mar;98(3):874–882. doi: 10.1042/bj0980874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Passonneau J. V., Lowry O. H. The role of phosphofructokinase in metabolic regulation. Adv Enzyme Regul. 1964;2:265–274. doi: 10.1016/s0065-2571(64)80018-2. [DOI] [PubMed] [Google Scholar]
  8. SALAS J., SALAS M., VINUELA E., SOLS A. GLUCOKINASE OF RABBIT LIVER. J Biol Chem. 1965 Mar;240:1014–1018. [PubMed] [Google Scholar]
  9. SALAS M., VINUELA E., SOLS A. INSULIN-DEPENDENT SYNTHESIS OF LIVER GLUCOKINASE IN THE RAT. J Biol Chem. 1963 Nov;238:3535–3538. [PubMed] [Google Scholar]
  10. SHARMA C., MANJESHWAR R., WEINHOUSE S. EFFECTS OF DIET AND INSULIN ON GLUCOSE-ADENOSINE TRIPHOSPHATE PHOSPHOTRANSFERASES OF RAT LIVER. J Biol Chem. 1963 Dec;238:3840–3845. [PubMed] [Google Scholar]
  11. SHARMA R. M., SHARMA C., DONNELLY A. J., MORRIS H. P., WEINHOUSE S. GLUCOSE-ATP PHOSPHOTRANSFERASES DURING HEPATOCARCINOGENESIS. Cancer Res. 1965 Feb;25:193–199. [PubMed] [Google Scholar]
  12. SHATTON J. B., DONNELLY A. J., WEINHOUSE S. Metabolism of neoplastic tissues. XVI. Glucokinase activity and glycogen levels during hepatocarcinogenesis by azo dyes. Cancer Res. 1962 Dec;22:1372–1380. [PubMed] [Google Scholar]
  13. Sharma C., Manjeshwar R., Weinhouse S. Hormonal and dietary regulation of hepatic glucokinase. Adv Enzyme Regul. 1964;2:189–200. doi: 10.1016/s0065-2571(64)80013-3. [DOI] [PubMed] [Google Scholar]
  14. Sols A., Salas M., Viñuela E. Induced biosynthesis of liver glucokinase. Adv Enzyme Regul. 1964;2:177–188. doi: 10.1016/s0065-2571(64)80012-1. [DOI] [PubMed] [Google Scholar]
  15. WALKER D. G. ON THE PRESENCE OF TWO SOLUBLE GLUCOSE-PHOSPHORYLATING ENZYMES IN ADULT LIVER AND THE DEVELOPMENT OF ONE OF THESE AFTER BIRTH. Biochim Biophys Acta. 1963 Oct 1;77:209–226. doi: 10.1016/0006-3002(63)90494-3. [DOI] [PubMed] [Google Scholar]
  16. Walker D. G., Holland G. The development of hepatic glucokinase in the neonatal rat. Biochem J. 1965 Dec;97(3):845–854. doi: 10.1042/bj0970845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Walker D. G., Rao S. The role of glucokinase in the phosphorylation of glucose by rat liver. Biochem J. 1964 Feb;90(2):360–368. doi: 10.1042/bj0900360. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES