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. 1988 May 15;138(10):917–919.

Evaluation of a kit for rapid detection of group A streptococci in a pediatric emergency department.

A M Mackenzie 1, M M Li 1, F T Chan 1
PMCID: PMC1267847  PMID: 3284627

Abstract

We evaluated a kit for the rapid detection of group A streptococci from throat swabs (Culturette Brand 10-Minute Group A Strep ID, Marion Scientific, Division of Marion Laboratories, Inc., Kansas City, Missouri) in the laboratory and in a busy pediatric emergency department. The sensitivity of the kit in the laboratory was 80% for all specimens and 94% for specimens with more than 10 colony-forming units of group A streptococci; the specificity was 99%. After initial training, emergency department pediatricians and nurses achieved sensitivities of 72% and 69% respectively. The specificity achieved by the pediatricians was 76% initially but 96% after further training. Untrained residents achieved a sensitivity of 58%. We conclude that this kit is potentially useful in the hands of adequately trained personnel, but without training the accuracy of the results is unacceptable. We recommend that the kit be used by designated staff trained and monitored by laboratory personnel.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Campos J. M., Charilaou C. C. Evaluation of Detect-A-Strep and the Culturette Ten-Minute Strep ID kits for detection of group A streptococcal antigen in oropharyngeal swabs from children. J Clin Microbiol. 1985 Aug;22(2):145–148. doi: 10.1128/jcm.22.2.145-148.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chang M. J., Mohla C. Ten-minute detection of group A streptococci in pediatric throat swabs. J Clin Microbiol. 1985 Feb;21(2):258–259. doi: 10.1128/jcm.21.2.258-259.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DuBois D., Ray V. G., Nelson B., Peacock J. B. Rapid diagnosis of group A strep pharyngitis in the emergency department. Ann Emerg Med. 1986 Feb;15(2):157–159. doi: 10.1016/s0196-0644(86)80011-7. [DOI] [PubMed] [Google Scholar]
  4. Gerber M. A. Culturing of throat swabs: end of an era? J Pediatr. 1985 Jul;107(1):85–88. doi: 10.1016/s0022-3476(85)80620-x. [DOI] [PubMed] [Google Scholar]
  5. Gerber M. A., Spadaccini L. J., Wright L. L., Deutsch L. Latex agglutination tests for rapid identification of group A streptococci directly from throat swabs. J Pediatr. 1984 Nov;105(5):702–705. doi: 10.1016/s0022-3476(84)80286-3. [DOI] [PubMed] [Google Scholar]
  6. Kelly M. T., Smith J. A., Jaffer S., Pearce L., Clarke A. Outpatient evaluation of a rapid, direct test for detection of group A streptococci in throat swabs. Am J Clin Pathol. 1987 Apr;87(4):522–525. doi: 10.1093/ajcp/87.4.522. [DOI] [PubMed] [Google Scholar]
  7. Lieu T. A., Fleisher G. R., Schwartz J. S. Clinical performance and effect on treatment rates of latex agglutination testing for streptococcal pharyngitis in an emergency department. Pediatr Infect Dis. 1986 Nov-Dec;5(6):655–659. doi: 10.1097/00006454-198611000-00011. [DOI] [PubMed] [Google Scholar]
  8. McCusker J. J., McCoy E. L., Young C. L., Alamares R., Hirsch L. S. Comparison of Directigen Group A Strep Test with a traditional culture technique for detection of group A beta-hemolytic streptococci. J Clin Microbiol. 1984 Oct;20(4):824–825. doi: 10.1128/jcm.20.4.824-825.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Radetsky M., Todd J. K. Criteria for the evaluation of new diagnostic tests. Pediatr Infect Dis. 1984 Sep-Oct;3(5):461–466. doi: 10.1097/00006454-198409000-00018. [DOI] [PubMed] [Google Scholar]
  10. Roddey O. F., Clegg H. W., Clardy L. T., Martin E. S., Swetenburg R. L. Comparison of a latex agglutination test and four culture methods for identification of group A streptococci in a pediatric office laboratory. J Pediatr. 1986 Mar;108(3):347–351. doi: 10.1016/s0022-3476(86)80871-x. [DOI] [PubMed] [Google Scholar]
  11. Schwartz R. H., Hayden G. F., McCoy P., Sait T., Chhabra O. Rapid diagnosis of streptococcal pharyngitis in two pediatric offices using a latex agglutination kit. Pediatr Infect Dis. 1985 Nov-Dec;4(6):647–650. doi: 10.1097/00006454-198511000-00010. [DOI] [PubMed] [Google Scholar]
  12. Veasy L. G., Wiedmeier S. E., Orsmond G. S., Ruttenberg H. D., Boucek M. M., Roth S. J., Tait V. F., Thompson J. A., Daly J. A., Kaplan E. L. Resurgence of acute rheumatic fever in the intermountain area of the United States. N Engl J Med. 1987 Feb 19;316(8):421–427. doi: 10.1056/NEJM198702193160801. [DOI] [PubMed] [Google Scholar]

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